Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2278 and EX28DRAFT_2279 are separated by 390 nucleotides EX28DRAFT_2279 and EX28DRAFT_2280 are separated by 398 nucleotides EX28DRAFT_2280 and EX28DRAFT_2281 are separated by 18 nucleotides
EX28DRAFT_2278: EX28DRAFT_2278 - Deoxyribodipyrimidine photolyase, at 2,300,908 to 2,302,320
_2278
EX28DRAFT_2279: EX28DRAFT_2279 - Protein of unknown function (DUF2517), at 2,302,711 to 2,302,917
_2279
EX28DRAFT_2280: EX28DRAFT_2280 - K+-transporting ATPase, KdpA, at 2,303,316 to 2,304,995
_2280
EX28DRAFT_2281: EX28DRAFT_2281 - K+-transporting ATPase, B subunit, at 2,305,014 to 2,307,062
_2281
Position (kb)
2303
2304
2305 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2302.321 kb on - strand at 2302.321 kb on - strand at 2302.333 kb on + strand at 2302.343 kb on + strand at 2302.343 kb on + strand at 2302.344 kb on - strand at 2302.344 kb on - strand at 2302.389 kb on + strand at 2302.406 kb on + strand at 2302.508 kb on - strand at 2302.546 kb on + strand at 2302.546 kb on + strand at 2302.546 kb on + strand at 2302.547 kb on - strand at 2302.587 kb on + strand at 2302.592 kb on - strand at 2302.592 kb on - strand at 2302.592 kb on - strand at 2302.592 kb on - strand at 2302.592 kb on - strand at 2302.592 kb on - strand at 2302.593 kb on + strand at 2302.594 kb on - strand at 2302.594 kb on - strand at 2302.601 kb on + strand at 2302.672 kb on - strand at 2302.688 kb on + strand at 2302.689 kb on - strand at 2302.694 kb on + strand at 2302.695 kb on - strand at 2302.695 kb on - strand at 2302.700 kb on + strand at 2302.701 kb on - strand at 2302.715 kb on + strand at 2302.793 kb on + strand, within EX28DRAFT_2279 at 2302.793 kb on + strand, within EX28DRAFT_2279 at 2302.794 kb on - strand, within EX28DRAFT_2279 at 2302.794 kb on - strand, within EX28DRAFT_2279 at 2302.794 kb on - strand, within EX28DRAFT_2279 at 2302.905 kb on + strand at 2302.905 kb on + strand at 2302.918 kb on - strand at 2302.932 kb on + strand at 2302.933 kb on - strand at 2302.933 kb on - strand at 2303.009 kb on - strand at 2303.052 kb on + strand at 2303.052 kb on + strand at 2303.141 kb on + strand at 2303.197 kb on - strand at 2303.197 kb on - strand at 2303.241 kb on + strand at 2303.287 kb on + strand at 2303.297 kb on + strand at 2303.353 kb on + strand at 2303.354 kb on - strand at 2303.470 kb on + strand at 2303.692 kb on - strand, within EX28DRAFT_2280 at 2303.834 kb on + strand, within EX28DRAFT_2280 at 2303.835 kb on - strand, within EX28DRAFT_2280 at 2303.925 kb on + strand, within EX28DRAFT_2280 at 2303.925 kb on + strand, within EX28DRAFT_2280 at 2303.925 kb on + strand, within EX28DRAFT_2280 at 2303.926 kb on - strand, within EX28DRAFT_2280 at 2303.926 kb on - strand, within EX28DRAFT_2280 at 2303.926 kb on - strand, within EX28DRAFT_2280 at 2303.927 kb on + strand, within EX28DRAFT_2280 at 2303.927 kb on + strand, within EX28DRAFT_2280 at 2303.972 kb on - strand, within EX28DRAFT_2280 at 2304.266 kb on + strand, within EX28DRAFT_2280 at 2304.267 kb on - strand, within EX28DRAFT_2280 at 2304.282 kb on - strand, within EX28DRAFT_2280 at 2304.318 kb on + strand, within EX28DRAFT_2280 at 2304.492 kb on - strand, within EX28DRAFT_2280 at 2304.526 kb on - strand, within EX28DRAFT_2280 at 2304.688 kb on - strand, within EX28DRAFT_2280 at 2304.944 kb on + strand at 2304.978 kb on + strand at 2304.978 kb on + strand at 2305.052 kb on - strand at 2305.236 kb on - strand, within EX28DRAFT_2281 at 2305.452 kb on - strand, within EX28DRAFT_2281 at 2305.586 kb on + strand, within EX28DRAFT_2281 at 2305.746 kb on + strand, within EX28DRAFT_2281 at 2305.947 kb on - strand, within EX28DRAFT_2281 at 2305.947 kb on - strand, within EX28DRAFT_2281 at 2305.949 kb on + strand, within EX28DRAFT_2281
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,302,321 - +0.5 2,302,321 - -1.1 2,302,333 + -0.1 2,302,343 + -1.4 2,302,343 + -1.2 2,302,344 - +0.7 2,302,344 - +0.4 2,302,389 + -0.2 2,302,406 + +0.2 2,302,508 - +0.2 2,302,546 + -1.0 2,302,546 + +1.1 2,302,546 + +0.7 2,302,547 - +0.7 2,302,587 + +1.5 2,302,592 - -0.4 2,302,592 - -0.8 2,302,592 - -1.5 2,302,592 - -0.4 2,302,592 - +0.4 2,302,592 - -0.1 2,302,593 + -1.6 2,302,594 - +0.1 2,302,594 - +1.9 2,302,601 + +0.7 2,302,672 - -0.4 2,302,688 + +0.3 2,302,689 - +0.8 2,302,694 + -0.1 2,302,695 - -1.3 2,302,695 - +0.1 2,302,700 + -0.2 2,302,701 - +0.8 2,302,715 + -0.0 2,302,793 + EX28DRAFT_2279 0.40 -0.2 2,302,793 + EX28DRAFT_2279 0.40 +0.6 2,302,794 - EX28DRAFT_2279 0.40 +0.1 2,302,794 - EX28DRAFT_2279 0.40 +0.1 2,302,794 - EX28DRAFT_2279 0.40 +0.3 2,302,905 + -0.0 2,302,905 + -0.2 2,302,918 - -1.6 2,302,932 + +0.0 2,302,933 - +1.3 2,302,933 - -0.2 2,303,009 - +0.4 2,303,052 + -1.1 2,303,052 + -0.2 2,303,141 + +0.5 2,303,197 - +0.5 2,303,197 - +0.0 2,303,241 + +0.3 2,303,287 + -1.4 2,303,297 + +0.9 2,303,353 + -0.0 2,303,354 - -0.1 2,303,470 + +0.9 2,303,692 - EX28DRAFT_2280 0.22 -0.1 2,303,834 + EX28DRAFT_2280 0.31 -0.6 2,303,835 - EX28DRAFT_2280 0.31 -0.2 2,303,925 + EX28DRAFT_2280 0.36 -0.8 2,303,925 + EX28DRAFT_2280 0.36 -0.9 2,303,925 + EX28DRAFT_2280 0.36 -1.0 2,303,926 - EX28DRAFT_2280 0.36 -1.6 2,303,926 - EX28DRAFT_2280 0.36 -0.5 2,303,926 - EX28DRAFT_2280 0.36 -1.2 2,303,927 + EX28DRAFT_2280 0.36 +0.3 2,303,927 + EX28DRAFT_2280 0.36 +0.5 2,303,972 - EX28DRAFT_2280 0.39 +0.1 2,304,266 + EX28DRAFT_2280 0.57 -3.1 2,304,267 - EX28DRAFT_2280 0.57 -0.1 2,304,282 - EX28DRAFT_2280 0.57 +1.1 2,304,318 + EX28DRAFT_2280 0.60 -1.0 2,304,492 - EX28DRAFT_2280 0.70 -0.2 2,304,526 - EX28DRAFT_2280 0.72 +0.6 2,304,688 - EX28DRAFT_2280 0.82 -1.5 2,304,944 + -1.2 2,304,978 + +0.7 2,304,978 + -0.3 2,305,052 - -0.8 2,305,236 - EX28DRAFT_2281 0.11 -0.7 2,305,452 - EX28DRAFT_2281 0.21 +0.1 2,305,586 + EX28DRAFT_2281 0.28 -0.3 2,305,746 + EX28DRAFT_2281 0.36 +0.3 2,305,947 - EX28DRAFT_2281 0.46 +0.3 2,305,947 - EX28DRAFT_2281 0.46 -0.6 2,305,949 + EX28DRAFT_2281 0.46 +0.7
Or see this region's nucleotide sequence