Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2109 and EX28DRAFT_2110 are separated by 171 nucleotides EX28DRAFT_2110 and EX28DRAFT_2111 overlap by 8 nucleotides EX28DRAFT_2111 and EX28DRAFT_2112 are separated by 273 nucleotides
EX28DRAFT_2109: EX28DRAFT_2109 - Reverse transcriptase (RNA-dependent DNA polymerase), at 2,131,057 to 2,132,040
_2109
EX28DRAFT_2110: EX28DRAFT_2110 - hypothetical protein, at 2,132,212 to 2,132,949
_2110
EX28DRAFT_2111: EX28DRAFT_2111 - hypothetical protein, at 2,132,942 to 2,133,487
_2111
EX28DRAFT_2112: EX28DRAFT_2112 - Integrase core domain, at 2,133,761 to 2,134,245
_2112
Position (kb)
2132
2133 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2131.254 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.506 kb on + strand, within EX28DRAFT_2109 at 2131.576 kb on - strand, within EX28DRAFT_2109 at 2132.054 kb on + strand at 2132.208 kb on - strand at 2132.281 kb on + strand at 2132.281 kb on + strand at 2132.313 kb on - strand, within EX28DRAFT_2110 at 2132.424 kb on + strand, within EX28DRAFT_2110 at 2132.695 kb on + strand, within EX28DRAFT_2110 at 2132.995 kb on + strand at 2133.257 kb on + strand, within EX28DRAFT_2111 at 2133.295 kb on + strand, within EX28DRAFT_2111 at 2133.296 kb on - strand, within EX28DRAFT_2111 at 2133.303 kb on - strand, within EX28DRAFT_2111 at 2133.345 kb on + strand, within EX28DRAFT_2111 at 2133.346 kb on - strand, within EX28DRAFT_2111 at 2133.346 kb on - strand, within EX28DRAFT_2111 at 2133.390 kb on + strand, within EX28DRAFT_2111 at 2133.409 kb on + strand, within EX28DRAFT_2111 at 2133.411 kb on + strand, within EX28DRAFT_2111 at 2133.453 kb on + strand at 2133.489 kb on + strand at 2133.497 kb on + strand at 2133.499 kb on + strand at 2133.588 kb on + strand at 2133.747 kb on - strand at 2133.749 kb on + strand at 2133.749 kb on + strand at 2133.749 kb on + strand at 2133.749 kb on + strand at 2133.749 kb on + strand at 2133.753 kb on + strand at 2133.753 kb on + strand at 2133.754 kb on - strand at 2133.767 kb on - strand at 2133.767 kb on - strand at 2133.767 kb on - strand at 2133.770 kb on - strand at 2133.785 kb on - strand at 2133.868 kb on + strand, within EX28DRAFT_2112 at 2133.870 kb on + strand, within EX28DRAFT_2112 at 2133.871 kb on - strand, within EX28DRAFT_2112
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,131,254 + EX28DRAFT_2109 0.20 +0.1 2,131,258 + EX28DRAFT_2109 0.20 +0.4 2,131,258 + EX28DRAFT_2109 0.20 +0.3 2,131,506 + EX28DRAFT_2109 0.46 +0.1 2,131,576 - EX28DRAFT_2109 0.53 -0.7 2,132,054 + -0.2 2,132,208 - +0.2 2,132,281 + -0.1 2,132,281 + +0.0 2,132,313 - EX28DRAFT_2110 0.14 -0.6 2,132,424 + EX28DRAFT_2110 0.29 -2.2 2,132,695 + EX28DRAFT_2110 0.65 -0.7 2,132,995 + -0.4 2,133,257 + EX28DRAFT_2111 0.58 -1.3 2,133,295 + EX28DRAFT_2111 0.65 +0.1 2,133,296 - EX28DRAFT_2111 0.65 +1.2 2,133,303 - EX28DRAFT_2111 0.66 -0.2 2,133,345 + EX28DRAFT_2111 0.74 +0.0 2,133,346 - EX28DRAFT_2111 0.74 +0.7 2,133,346 - EX28DRAFT_2111 0.74 -0.9 2,133,390 + EX28DRAFT_2111 0.82 -0.9 2,133,409 + EX28DRAFT_2111 0.86 -0.9 2,133,411 + EX28DRAFT_2111 0.86 -1.5 2,133,453 + +1.8 2,133,489 + -0.4 2,133,497 + -0.5 2,133,499 + +0.0 2,133,588 + -0.0 2,133,747 - -0.6 2,133,749 + +0.3 2,133,749 + -0.5 2,133,749 + -0.8 2,133,749 + -0.1 2,133,749 + -0.3 2,133,753 + -0.0 2,133,753 + -0.7 2,133,754 - +0.4 2,133,767 - -1.0 2,133,767 - +1.4 2,133,767 - +0.9 2,133,770 - -0.3 2,133,785 - -2.0 2,133,868 + EX28DRAFT_2112 0.22 +0.3 2,133,870 + EX28DRAFT_2112 0.22 -0.4 2,133,871 - EX28DRAFT_2112 0.23 -1.0
Or see this region's nucleotide sequence