Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1925 and EX28DRAFT_1926 are separated by 152 nucleotides EX28DRAFT_1926 and EX28DRAFT_1927 are separated by 125 nucleotides EX28DRAFT_1927 and EX28DRAFT_1928 are separated by 1 nucleotides EX28DRAFT_1928 and EX28DRAFT_1929 are separated by 100 nucleotides
EX28DRAFT_1925: EX28DRAFT_1925 - Histidine phosphatase superfamily (branch 2), at 1,933,367 to 1,934,608
_1925
EX28DRAFT_1926: EX28DRAFT_1926 - transcriptional regulator, HxlR family, at 1,934,761 to 1,935,204
_1926
EX28DRAFT_1927: EX28DRAFT_1927 - Short-chain dehydrogenases of various substrate specificities, at 1,935,330 to 1,936,124
_1927
EX28DRAFT_1928: EX28DRAFT_1928 - transcriptional regulator, HxlR family, at 1,936,126 to 1,936,485
_1928
EX28DRAFT_1929: EX28DRAFT_1929 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,936,586 to 1,937,335
_1929
Position (kb)
1935
1936
1937 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1934.355 kb on + strand, within EX28DRAFT_1925 at 1934.355 kb on + strand, within EX28DRAFT_1925 at 1934.356 kb on - strand, within EX28DRAFT_1925 at 1934.356 kb on - strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.367 kb on + strand, within EX28DRAFT_1925 at 1934.368 kb on - strand, within EX28DRAFT_1925 at 1934.507 kb on + strand at 1934.507 kb on + strand at 1934.508 kb on - strand at 1934.523 kb on + strand at 1934.612 kb on + strand at 1934.613 kb on - strand at 1934.714 kb on + strand at 1934.770 kb on - strand at 1934.998 kb on + strand, within EX28DRAFT_1926 at 1934.998 kb on + strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1934.999 kb on - strand, within EX28DRAFT_1926 at 1935.322 kb on - strand at 1935.581 kb on + strand, within EX28DRAFT_1927 at 1935.654 kb on - strand, within EX28DRAFT_1927 at 1935.654 kb on - strand, within EX28DRAFT_1927 at 1935.657 kb on - strand, within EX28DRAFT_1927 at 1935.694 kb on - strand, within EX28DRAFT_1927 at 1935.789 kb on + strand, within EX28DRAFT_1927 at 1935.801 kb on - strand, within EX28DRAFT_1927 at 1935.965 kb on + strand, within EX28DRAFT_1927 at 1936.122 kb on + strand at 1936.437 kb on + strand, within EX28DRAFT_1928 at 1936.437 kb on + strand, within EX28DRAFT_1928 at 1936.437 kb on + strand, within EX28DRAFT_1928 at 1936.438 kb on - strand, within EX28DRAFT_1928 at 1936.439 kb on + strand, within EX28DRAFT_1928 at 1936.440 kb on - strand, within EX28DRAFT_1928 at 1936.549 kb on + strand at 1936.549 kb on + strand at 1936.549 kb on + strand at 1936.550 kb on - strand at 1936.550 kb on - strand at 1936.550 kb on - strand at 1936.551 kb on + strand at 1936.552 kb on - strand at 1936.603 kb on + strand at 1936.603 kb on + strand at 1936.603 kb on + strand at 1936.604 kb on - strand at 1936.607 kb on + strand at 1936.609 kb on + strand at 1936.609 kb on + strand at 1936.609 kb on + strand at 1936.610 kb on - strand at 1936.729 kb on + strand, within EX28DRAFT_1929 at 1936.730 kb on - strand, within EX28DRAFT_1929 at 1936.730 kb on - strand, within EX28DRAFT_1929 at 1936.730 kb on - strand, within EX28DRAFT_1929 at 1936.938 kb on + strand, within EX28DRAFT_1929 at 1937.034 kb on - strand, within EX28DRAFT_1929 at 1937.034 kb on - strand, within EX28DRAFT_1929
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,934,355 + EX28DRAFT_1925 0.80 +0.3 1,934,355 + EX28DRAFT_1925 0.80 -0.4 1,934,356 - EX28DRAFT_1925 0.80 +1.0 1,934,356 - EX28DRAFT_1925 0.80 +1.8 1,934,367 + EX28DRAFT_1925 0.81 -0.1 1,934,367 + EX28DRAFT_1925 0.81 -1.8 1,934,367 + EX28DRAFT_1925 0.81 -0.7 1,934,367 + EX28DRAFT_1925 0.81 +0.1 1,934,368 - EX28DRAFT_1925 0.81 +0.9 1,934,507 + +0.7 1,934,507 + +1.0 1,934,508 - +0.2 1,934,523 + -0.4 1,934,612 + +0.5 1,934,613 - -0.9 1,934,714 + +0.3 1,934,770 - -0.4 1,934,998 + EX28DRAFT_1926 0.53 -0.4 1,934,998 + EX28DRAFT_1926 0.53 +0.6 1,934,999 - EX28DRAFT_1926 0.54 +0.1 1,934,999 - EX28DRAFT_1926 0.54 +1.2 1,934,999 - EX28DRAFT_1926 0.54 -0.2 1,935,322 - -1.8 1,935,581 + EX28DRAFT_1927 0.32 +1.0 1,935,654 - EX28DRAFT_1927 0.41 +0.1 1,935,654 - EX28DRAFT_1927 0.41 -0.5 1,935,657 - EX28DRAFT_1927 0.41 -0.3 1,935,694 - EX28DRAFT_1927 0.46 -0.0 1,935,789 + EX28DRAFT_1927 0.58 -1.3 1,935,801 - EX28DRAFT_1927 0.59 +1.2 1,935,965 + EX28DRAFT_1927 0.80 -0.8 1,936,122 + +0.0 1,936,437 + EX28DRAFT_1928 0.86 +0.3 1,936,437 + EX28DRAFT_1928 0.86 +0.1 1,936,437 + EX28DRAFT_1928 0.86 -0.8 1,936,438 - EX28DRAFT_1928 0.87 -0.3 1,936,439 + EX28DRAFT_1928 0.87 -0.5 1,936,440 - EX28DRAFT_1928 0.87 +0.1 1,936,549 + -0.3 1,936,549 + +0.1 1,936,549 + -0.4 1,936,550 - -0.8 1,936,550 - +0.8 1,936,550 - -0.2 1,936,551 + -1.4 1,936,552 - -0.4 1,936,603 + -2.6 1,936,603 + +0.9 1,936,603 + -0.2 1,936,604 - +0.3 1,936,607 + +0.1 1,936,609 + -0.2 1,936,609 + -0.2 1,936,609 + +2.6 1,936,610 - +0.1 1,936,729 + EX28DRAFT_1929 0.19 -1.0 1,936,730 - EX28DRAFT_1929 0.19 +3.3 1,936,730 - EX28DRAFT_1929 0.19 -0.1 1,936,730 - EX28DRAFT_1929 0.19 +0.3 1,936,938 + EX28DRAFT_1929 0.47 -0.5 1,937,034 - EX28DRAFT_1929 0.60 -2.7 1,937,034 - EX28DRAFT_1929 0.60 -0.4
Or see this region's nucleotide sequence