Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1526 and EX28DRAFT_1527 are separated by 82 nucleotides EX28DRAFT_1527 and EX28DRAFT_1528 are separated by 12 nucleotides
EX28DRAFT_1526: EX28DRAFT_1526 - Transcriptional regulator, at 1,542,168 to 1,543,055
_1526
EX28DRAFT_1527: EX28DRAFT_1527 - hypothetical protein, at 1,543,138 to 1,543,407
_1527
EX28DRAFT_1528: EX28DRAFT_1528 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 1,543,420 to 1,544,682
_1528
Position (kb)
1543
1544 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1542.176 kb on + strand at 1542.176 kb on + strand at 1542.194 kb on + strand at 1542.435 kb on - strand, within EX28DRAFT_1526 at 1542.724 kb on + strand, within EX28DRAFT_1526 at 1542.725 kb on - strand, within EX28DRAFT_1526 at 1542.957 kb on + strand, within EX28DRAFT_1526 at 1543.015 kb on + strand at 1543.016 kb on - strand at 1543.039 kb on + strand at 1543.100 kb on - strand at 1543.137 kb on + strand at 1543.137 kb on + strand at 1543.138 kb on - strand at 1543.138 kb on - strand at 1543.309 kb on - strand, within EX28DRAFT_1527 at 1543.324 kb on + strand, within EX28DRAFT_1527 at 1543.324 kb on + strand, within EX28DRAFT_1527 at 1543.422 kb on - strand at 1543.422 kb on - strand at 1543.456 kb on - strand at 1543.458 kb on - strand at 1543.522 kb on + strand at 1543.522 kb on + strand at 1543.523 kb on - strand at 1543.523 kb on - strand at 1543.523 kb on - strand at 1543.546 kb on + strand at 1543.546 kb on + strand at 1543.547 kb on - strand, within EX28DRAFT_1528 at 1543.547 kb on - strand, within EX28DRAFT_1528 at 1543.547 kb on - strand, within EX28DRAFT_1528 at 1543.666 kb on - strand, within EX28DRAFT_1528 at 1543.667 kb on + strand, within EX28DRAFT_1528 at 1543.667 kb on + strand, within EX28DRAFT_1528 at 1543.668 kb on - strand, within EX28DRAFT_1528 at 1543.668 kb on - strand, within EX28DRAFT_1528 at 1543.688 kb on - strand, within EX28DRAFT_1528 at 1543.689 kb on + strand, within EX28DRAFT_1528 at 1543.689 kb on + strand, within EX28DRAFT_1528 at 1543.689 kb on + strand, within EX28DRAFT_1528 at 1543.689 kb on + strand, within EX28DRAFT_1528 at 1543.689 kb on + strand, within EX28DRAFT_1528 at 1543.690 kb on - strand, within EX28DRAFT_1528 at 1543.690 kb on - strand, within EX28DRAFT_1528 at 1543.690 kb on - strand, within EX28DRAFT_1528 at 1543.824 kb on + strand, within EX28DRAFT_1528 at 1543.835 kb on + strand, within EX28DRAFT_1528 at 1543.915 kb on - strand, within EX28DRAFT_1528 at 1543.917 kb on + strand, within EX28DRAFT_1528 at 1543.917 kb on + strand, within EX28DRAFT_1528 at 1544.231 kb on + strand, within EX28DRAFT_1528 at 1544.231 kb on + strand, within EX28DRAFT_1528 at 1544.231 kb on + strand, within EX28DRAFT_1528 at 1544.232 kb on - strand, within EX28DRAFT_1528 at 1544.391 kb on - strand, within EX28DRAFT_1528
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,542,176 + -0.0 1,542,176 + +0.2 1,542,194 + +0.3 1,542,435 - EX28DRAFT_1526 0.30 +0.2 1,542,724 + EX28DRAFT_1526 0.63 +0.2 1,542,725 - EX28DRAFT_1526 0.63 +1.3 1,542,957 + EX28DRAFT_1526 0.89 +0.4 1,543,015 + -1.1 1,543,016 - +1.5 1,543,039 + +0.3 1,543,100 - +0.2 1,543,137 + +0.5 1,543,137 + +0.7 1,543,138 - +0.0 1,543,138 - -0.2 1,543,309 - EX28DRAFT_1527 0.63 -2.0 1,543,324 + EX28DRAFT_1527 0.69 -0.9 1,543,324 + EX28DRAFT_1527 0.69 -0.3 1,543,422 - -0.8 1,543,422 - +0.0 1,543,456 - +0.0 1,543,458 - +1.1 1,543,522 + +0.5 1,543,522 + +0.9 1,543,523 - +0.3 1,543,523 - +0.0 1,543,523 - +0.4 1,543,546 + +0.3 1,543,546 + -0.6 1,543,547 - EX28DRAFT_1528 0.10 -0.4 1,543,547 - EX28DRAFT_1528 0.10 -0.7 1,543,547 - EX28DRAFT_1528 0.10 +0.0 1,543,666 - EX28DRAFT_1528 0.19 +0.3 1,543,667 + EX28DRAFT_1528 0.20 -0.0 1,543,667 + EX28DRAFT_1528 0.20 -1.2 1,543,668 - EX28DRAFT_1528 0.20 -0.2 1,543,668 - EX28DRAFT_1528 0.20 -1.1 1,543,688 - EX28DRAFT_1528 0.21 +0.3 1,543,689 + EX28DRAFT_1528 0.21 +1.4 1,543,689 + EX28DRAFT_1528 0.21 -0.1 1,543,689 + EX28DRAFT_1528 0.21 +0.5 1,543,689 + EX28DRAFT_1528 0.21 -1.2 1,543,689 + EX28DRAFT_1528 0.21 +1.2 1,543,690 - EX28DRAFT_1528 0.21 -1.4 1,543,690 - EX28DRAFT_1528 0.21 +0.6 1,543,690 - EX28DRAFT_1528 0.21 +1.6 1,543,824 + EX28DRAFT_1528 0.32 -0.1 1,543,835 + EX28DRAFT_1528 0.33 +0.0 1,543,915 - EX28DRAFT_1528 0.39 +2.2 1,543,917 + EX28DRAFT_1528 0.39 +1.7 1,543,917 + EX28DRAFT_1528 0.39 +0.3 1,544,231 + EX28DRAFT_1528 0.64 +0.0 1,544,231 + EX28DRAFT_1528 0.64 -0.1 1,544,231 + EX28DRAFT_1528 0.64 +0.4 1,544,232 - EX28DRAFT_1528 0.64 -0.5 1,544,391 - EX28DRAFT_1528 0.77 +0.2
Or see this region's nucleotide sequence