Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1229 and EX28DRAFT_1230 overlap by 4 nucleotides EX28DRAFT_1230 and EX28DRAFT_1231 are separated by 135 nucleotides
EX28DRAFT_1229: EX28DRAFT_1229 - peptidase T, at 1,247,579 to 1,248,808
_1229
EX28DRAFT_1230: EX28DRAFT_1230 - tellurite resistance protein TehB, at 1,248,805 to 1,249,398
_1230
EX28DRAFT_1231: EX28DRAFT_1231 - Bacterial transferase hexapeptide (six repeats), at 1,249,534 to 1,250,514
_1231
Position (kb)
1248
1249
1250 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1247.815 kb on + strand, within EX28DRAFT_1229 at 1248.013 kb on + strand, within EX28DRAFT_1229 at 1248.368 kb on + strand, within EX28DRAFT_1229 at 1248.368 kb on + strand, within EX28DRAFT_1229 at 1248.369 kb on - strand, within EX28DRAFT_1229 at 1248.369 kb on - strand, within EX28DRAFT_1229 at 1248.369 kb on - strand, within EX28DRAFT_1229 at 1248.370 kb on + strand, within EX28DRAFT_1229 at 1248.371 kb on - strand, within EX28DRAFT_1229 at 1248.538 kb on + strand, within EX28DRAFT_1229 at 1248.539 kb on - strand, within EX28DRAFT_1229 at 1248.542 kb on + strand, within EX28DRAFT_1229 at 1248.542 kb on + strand, within EX28DRAFT_1229 at 1248.543 kb on - strand, within EX28DRAFT_1229 at 1248.550 kb on + strand, within EX28DRAFT_1229 at 1248.924 kb on - strand, within EX28DRAFT_1230 at 1248.927 kb on - strand, within EX28DRAFT_1230 at 1249.115 kb on + strand, within EX28DRAFT_1230 at 1249.149 kb on + strand, within EX28DRAFT_1230 at 1249.163 kb on - strand, within EX28DRAFT_1230 at 1249.365 kb on - strand at 1249.379 kb on + strand at 1249.380 kb on - strand at 1249.420 kb on - strand at 1249.505 kb on + strand at 1249.509 kb on + strand at 1249.510 kb on - strand at 1249.529 kb on + strand at 1249.594 kb on - strand at 1249.807 kb on - strand, within EX28DRAFT_1231 at 1249.997 kb on - strand, within EX28DRAFT_1231 at 1250.129 kb on + strand, within EX28DRAFT_1231 at 1250.129 kb on + strand, within EX28DRAFT_1231 at 1250.129 kb on + strand, within EX28DRAFT_1231 at 1250.130 kb on - strand, within EX28DRAFT_1231 at 1250.133 kb on + strand, within EX28DRAFT_1231 at 1250.133 kb on + strand, within EX28DRAFT_1231 at 1250.134 kb on - strand, within EX28DRAFT_1231 at 1250.164 kb on + strand, within EX28DRAFT_1231 at 1250.165 kb on - strand, within EX28DRAFT_1231
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,247,815 + EX28DRAFT_1229 0.19 -0.5 1,248,013 + EX28DRAFT_1229 0.35 +0.5 1,248,368 + EX28DRAFT_1229 0.64 -0.4 1,248,368 + EX28DRAFT_1229 0.64 -0.5 1,248,369 - EX28DRAFT_1229 0.64 +1.1 1,248,369 - EX28DRAFT_1229 0.64 -0.2 1,248,369 - EX28DRAFT_1229 0.64 +0.0 1,248,370 + EX28DRAFT_1229 0.64 +0.3 1,248,371 - EX28DRAFT_1229 0.64 +0.0 1,248,538 + EX28DRAFT_1229 0.78 -0.3 1,248,539 - EX28DRAFT_1229 0.78 +0.9 1,248,542 + EX28DRAFT_1229 0.78 -0.0 1,248,542 + EX28DRAFT_1229 0.78 -3.0 1,248,543 - EX28DRAFT_1229 0.78 +1.3 1,248,550 + EX28DRAFT_1229 0.79 +0.1 1,248,924 - EX28DRAFT_1230 0.20 -0.1 1,248,927 - EX28DRAFT_1230 0.21 -1.8 1,249,115 + EX28DRAFT_1230 0.52 -1.4 1,249,149 + EX28DRAFT_1230 0.58 +2.4 1,249,163 - EX28DRAFT_1230 0.60 -1.2 1,249,365 - -0.1 1,249,379 + +0.3 1,249,380 - -0.1 1,249,420 - +0.0 1,249,505 + -2.0 1,249,509 + -0.1 1,249,510 - +0.1 1,249,529 + -0.5 1,249,594 - -0.2 1,249,807 - EX28DRAFT_1231 0.28 +0.3 1,249,997 - EX28DRAFT_1231 0.47 +0.9 1,250,129 + EX28DRAFT_1231 0.61 -0.2 1,250,129 + EX28DRAFT_1231 0.61 -0.6 1,250,129 + EX28DRAFT_1231 0.61 -1.0 1,250,130 - EX28DRAFT_1231 0.61 +1.1 1,250,133 + EX28DRAFT_1231 0.61 +0.2 1,250,133 + EX28DRAFT_1231 0.61 -0.3 1,250,134 - EX28DRAFT_1231 0.61 -0.7 1,250,164 + EX28DRAFT_1231 0.64 +0.2 1,250,165 - EX28DRAFT_1231 0.64 +0.4
Or see this region's nucleotide sequence