Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1159 and EX28DRAFT_1160 overlap by 4 nucleotides EX28DRAFT_1160 and EX28DRAFT_1161 are separated by 9 nucleotides EX28DRAFT_1161 and EX28DRAFT_1162 are separated by 32 nucleotides
EX28DRAFT_1159: EX28DRAFT_1159 - Predicted membrane protein, at 1,179,885 to 1,180,607
_1159
EX28DRAFT_1160: EX28DRAFT_1160 - Predicted membrane protein, at 1,180,604 to 1,181,590
_1160
EX28DRAFT_1161: EX28DRAFT_1161 - Protein of unknown function (DUF2891), at 1,181,600 to 1,182,595
_1161
EX28DRAFT_1162: EX28DRAFT_1162 - Major Facilitator Superfamily, at 1,182,628 to 1,184,043
_1162
Position (kb)
1181
1182
1183 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1180.638 kb on - strand at 1180.868 kb on - strand, within EX28DRAFT_1160 at 1180.868 kb on - strand, within EX28DRAFT_1160 at 1180.868 kb on - strand, within EX28DRAFT_1160 at 1180.868 kb on - strand, within EX28DRAFT_1160 at 1181.187 kb on + strand, within EX28DRAFT_1160 at 1181.204 kb on - strand, within EX28DRAFT_1160 at 1181.368 kb on + strand, within EX28DRAFT_1160 at 1181.500 kb on + strand at 1181.501 kb on - strand at 1181.501 kb on - strand at 1181.504 kb on + strand at 1181.505 kb on - strand at 1181.505 kb on - strand at 1181.505 kb on - strand at 1181.570 kb on + strand at 1181.571 kb on - strand at 1181.571 kb on - strand at 1181.651 kb on + strand at 1181.651 kb on + strand at 1181.667 kb on - strand at 1181.892 kb on - strand, within EX28DRAFT_1161 at 1182.054 kb on - strand, within EX28DRAFT_1161 at 1182.095 kb on + strand, within EX28DRAFT_1161 at 1182.194 kb on + strand, within EX28DRAFT_1161 at 1182.245 kb on + strand, within EX28DRAFT_1161 at 1182.245 kb on + strand, within EX28DRAFT_1161 at 1182.245 kb on + strand, within EX28DRAFT_1161 at 1182.442 kb on + strand, within EX28DRAFT_1161 at 1182.443 kb on - strand, within EX28DRAFT_1161 at 1182.448 kb on + strand, within EX28DRAFT_1161 at 1182.545 kb on + strand at 1182.546 kb on - strand at 1182.775 kb on + strand, within EX28DRAFT_1162 at 1182.842 kb on + strand, within EX28DRAFT_1162 at 1182.842 kb on + strand, within EX28DRAFT_1162 at 1182.943 kb on - strand, within EX28DRAFT_1162 at 1183.133 kb on + strand, within EX28DRAFT_1162 at 1183.133 kb on + strand, within EX28DRAFT_1162 at 1183.284 kb on + strand, within EX28DRAFT_1162 at 1183.377 kb on + strand, within EX28DRAFT_1162 at 1183.544 kb on - strand, within EX28DRAFT_1162
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,180,638 - +1.0 1,180,868 - EX28DRAFT_1160 0.27 -1.8 1,180,868 - EX28DRAFT_1160 0.27 -0.5 1,180,868 - EX28DRAFT_1160 0.27 -1.0 1,180,868 - EX28DRAFT_1160 0.27 -0.5 1,181,187 + EX28DRAFT_1160 0.59 -0.4 1,181,204 - EX28DRAFT_1160 0.61 -0.4 1,181,368 + EX28DRAFT_1160 0.77 -0.1 1,181,500 + +0.7 1,181,501 - -0.5 1,181,501 - -0.4 1,181,504 + -0.0 1,181,505 - -0.2 1,181,505 - +0.2 1,181,505 - -2.2 1,181,570 + -1.2 1,181,571 - -3.9 1,181,571 - +0.2 1,181,651 + +0.4 1,181,651 + +0.6 1,181,667 - -0.7 1,181,892 - EX28DRAFT_1161 0.29 -0.5 1,182,054 - EX28DRAFT_1161 0.46 +0.1 1,182,095 + EX28DRAFT_1161 0.50 -0.8 1,182,194 + EX28DRAFT_1161 0.60 -2.8 1,182,245 + EX28DRAFT_1161 0.65 +2.1 1,182,245 + EX28DRAFT_1161 0.65 +0.2 1,182,245 + EX28DRAFT_1161 0.65 -0.2 1,182,442 + EX28DRAFT_1161 0.85 +0.3 1,182,443 - EX28DRAFT_1161 0.85 -0.8 1,182,448 + EX28DRAFT_1161 0.85 -0.4 1,182,545 + +0.4 1,182,546 - +0.2 1,182,775 + EX28DRAFT_1162 0.10 +0.6 1,182,842 + EX28DRAFT_1162 0.15 -0.7 1,182,842 + EX28DRAFT_1162 0.15 +0.5 1,182,943 - EX28DRAFT_1162 0.22 -0.9 1,183,133 + EX28DRAFT_1162 0.36 -1.0 1,183,133 + EX28DRAFT_1162 0.36 +0.3 1,183,284 + EX28DRAFT_1162 0.46 +0.4 1,183,377 + EX28DRAFT_1162 0.53 +0.2 1,183,544 - EX28DRAFT_1162 0.65 -1.3
Or see this region's nucleotide sequence