Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1108 and EX28DRAFT_1109 are separated by 114 nucleotides EX28DRAFT_1109 and EX28DRAFT_1110 are separated by 23 nucleotides EX28DRAFT_1110 and EX28DRAFT_1111 are separated by 133 nucleotides EX28DRAFT_1111 and EX28DRAFT_1112 overlap by 4 nucleotides
EX28DRAFT_1108: EX28DRAFT_1108 - hypothetical protein, at 1,121,223 to 1,122,182
_1108
EX28DRAFT_1109: EX28DRAFT_1109 - peroxiredoxin, OsmC subfamily, at 1,122,297 to 1,122,725
_1109
EX28DRAFT_1110: EX28DRAFT_1110 - Transcriptional regulator, at 1,122,749 to 1,123,654
_1110
EX28DRAFT_1111: EX28DRAFT_1111 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,123,788 to 1,124,537
_1111
EX28DRAFT_1112: EX28DRAFT_1112 - oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain, at 1,124,534 to 1,125,463
_1112
Position (kb)
1122
1123
1124 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1121.881 kb on + strand, within EX28DRAFT_1108 at 1121.921 kb on + strand, within EX28DRAFT_1108 at 1121.921 kb on + strand, within EX28DRAFT_1108 at 1121.922 kb on - strand, within EX28DRAFT_1108 at 1121.922 kb on - strand, within EX28DRAFT_1108 at 1121.983 kb on + strand, within EX28DRAFT_1108 at 1121.983 kb on + strand, within EX28DRAFT_1108 at 1121.983 kb on + strand, within EX28DRAFT_1108 at 1121.984 kb on - strand, within EX28DRAFT_1108 at 1122.092 kb on + strand at 1122.093 kb on - strand at 1122.094 kb on + strand at 1122.095 kb on - strand at 1122.095 kb on - strand at 1122.095 kb on - strand at 1122.095 kb on - strand at 1122.095 kb on - strand at 1122.098 kb on + strand at 1122.098 kb on + strand at 1122.099 kb on - strand at 1122.183 kb on - strand at 1122.255 kb on + strand at 1122.255 kb on + strand at 1122.255 kb on + strand at 1122.256 kb on - strand at 1122.260 kb on + strand at 1122.261 kb on - strand at 1122.261 kb on - strand at 1122.261 kb on - strand at 1122.262 kb on + strand at 1122.262 kb on + strand at 1122.262 kb on + strand at 1122.263 kb on - strand at 1122.263 kb on - strand at 1122.263 kb on - strand at 1122.263 kb on - strand at 1122.263 kb on - strand at 1122.266 kb on + strand at 1122.266 kb on + strand at 1122.267 kb on - strand at 1122.302 kb on + strand at 1122.302 kb on + strand at 1122.302 kb on + strand at 1122.342 kb on - strand, within EX28DRAFT_1109 at 1122.534 kb on - strand, within EX28DRAFT_1109 at 1122.648 kb on - strand, within EX28DRAFT_1109 at 1122.711 kb on + strand at 1122.711 kb on + strand at 1122.711 kb on + strand at 1122.712 kb on - strand at 1122.713 kb on + strand at 1122.720 kb on - strand at 1122.723 kb on + strand at 1122.724 kb on - strand at 1122.724 kb on - strand at 1122.793 kb on + strand at 1122.794 kb on - strand at 1122.794 kb on - strand at 1122.794 kb on - strand at 1122.802 kb on + strand at 1122.976 kb on + strand, within EX28DRAFT_1110 at 1122.977 kb on - strand, within EX28DRAFT_1110 at 1122.996 kb on + strand, within EX28DRAFT_1110 at 1122.996 kb on + strand, within EX28DRAFT_1110 at 1122.996 kb on + strand, within EX28DRAFT_1110 at 1123.077 kb on + strand, within EX28DRAFT_1110 at 1123.078 kb on - strand, within EX28DRAFT_1110 at 1123.082 kb on - strand, within EX28DRAFT_1110 at 1123.238 kb on + strand, within EX28DRAFT_1110 at 1123.239 kb on - strand, within EX28DRAFT_1110 at 1123.313 kb on - strand, within EX28DRAFT_1110 at 1123.462 kb on - strand, within EX28DRAFT_1110 at 1123.462 kb on - strand, within EX28DRAFT_1110 at 1123.518 kb on - strand, within EX28DRAFT_1110 at 1123.573 kb on + strand at 1123.597 kb on + strand at 1123.781 kb on - strand at 1123.781 kb on - strand at 1123.890 kb on + strand, within EX28DRAFT_1111 at 1123.891 kb on - strand, within EX28DRAFT_1111 at 1123.919 kb on - strand, within EX28DRAFT_1111
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,121,881 + EX28DRAFT_1108 0.69 +0.1 1,121,921 + EX28DRAFT_1108 0.73 -0.8 1,121,921 + EX28DRAFT_1108 0.73 +0.2 1,121,922 - EX28DRAFT_1108 0.73 -0.1 1,121,922 - EX28DRAFT_1108 0.73 +0.8 1,121,983 + EX28DRAFT_1108 0.79 -0.9 1,121,983 + EX28DRAFT_1108 0.79 +0.8 1,121,983 + EX28DRAFT_1108 0.79 -0.5 1,121,984 - EX28DRAFT_1108 0.79 -0.4 1,122,092 + -0.1 1,122,093 - +0.4 1,122,094 + -1.7 1,122,095 - +0.1 1,122,095 - -0.4 1,122,095 - +0.3 1,122,095 - -0.9 1,122,095 - -1.0 1,122,098 + +1.3 1,122,098 + -0.2 1,122,099 - -0.1 1,122,183 - -0.8 1,122,255 + +0.5 1,122,255 + -2.1 1,122,255 + +0.5 1,122,256 - +0.8 1,122,260 + +0.4 1,122,261 - +1.1 1,122,261 - +1.0 1,122,261 - -0.0 1,122,262 + -0.7 1,122,262 + +0.4 1,122,262 + -0.3 1,122,263 - -0.4 1,122,263 - -0.5 1,122,263 - -0.2 1,122,263 - -0.8 1,122,263 - -2.1 1,122,266 + +0.1 1,122,266 + +0.5 1,122,267 - +0.2 1,122,302 + -0.8 1,122,302 + +0.2 1,122,302 + -0.8 1,122,342 - EX28DRAFT_1109 0.10 -0.8 1,122,534 - EX28DRAFT_1109 0.55 +0.3 1,122,648 - EX28DRAFT_1109 0.82 -0.9 1,122,711 + +0.6 1,122,711 + +0.9 1,122,711 + +0.9 1,122,712 - -1.0 1,122,713 + +0.2 1,122,720 - -0.4 1,122,723 + +0.2 1,122,724 - -0.1 1,122,724 - -2.9 1,122,793 + -0.7 1,122,794 - -0.0 1,122,794 - +1.3 1,122,794 - +1.2 1,122,802 + +1.3 1,122,976 + EX28DRAFT_1110 0.25 +0.7 1,122,977 - EX28DRAFT_1110 0.25 -0.6 1,122,996 + EX28DRAFT_1110 0.27 +0.3 1,122,996 + EX28DRAFT_1110 0.27 +0.0 1,122,996 + EX28DRAFT_1110 0.27 -0.7 1,123,077 + EX28DRAFT_1110 0.36 +0.9 1,123,078 - EX28DRAFT_1110 0.36 +0.6 1,123,082 - EX28DRAFT_1110 0.37 +0.7 1,123,238 + EX28DRAFT_1110 0.54 +0.3 1,123,239 - EX28DRAFT_1110 0.54 +0.2 1,123,313 - EX28DRAFT_1110 0.62 +0.9 1,123,462 - EX28DRAFT_1110 0.79 +0.5 1,123,462 - EX28DRAFT_1110 0.79 -0.6 1,123,518 - EX28DRAFT_1110 0.85 +0.8 1,123,573 + -0.7 1,123,597 + -0.2 1,123,781 - +1.2 1,123,781 - -0.6 1,123,890 + EX28DRAFT_1111 0.14 +0.6 1,123,891 - EX28DRAFT_1111 0.14 -0.4 1,123,919 - EX28DRAFT_1111 0.17 -0.5
Or see this region's nucleotide sequence