Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1061 and EX28DRAFT_1062 are separated by 32 nucleotides EX28DRAFT_1062 and EX28DRAFT_1063 are separated by 404 nucleotides
EX28DRAFT_1061: EX28DRAFT_1061 - Malic enzyme, at 1,071,339 to 1,073,024
_1061
EX28DRAFT_1062: EX28DRAFT_1062 - Predicted permeases, at 1,073,057 to 1,074,004
_1062
EX28DRAFT_1063: EX28DRAFT_1063 - Na+/citrate symporter, at 1,074,409 to 1,075,740
_1063
Position (kb)
1073
1074
1075 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1072.227 kb on - strand, within EX28DRAFT_1061 at 1072.353 kb on - strand, within EX28DRAFT_1061 at 1072.353 kb on - strand, within EX28DRAFT_1061 at 1072.409 kb on - strand, within EX28DRAFT_1061 at 1072.409 kb on - strand, within EX28DRAFT_1061 at 1072.541 kb on + strand, within EX28DRAFT_1061 at 1072.560 kb on + strand, within EX28DRAFT_1061 at 1072.566 kb on - strand, within EX28DRAFT_1061 at 1072.726 kb on + strand, within EX28DRAFT_1061 at 1072.726 kb on + strand, within EX28DRAFT_1061 at 1072.727 kb on - strand, within EX28DRAFT_1061 at 1072.836 kb on - strand, within EX28DRAFT_1061 at 1072.956 kb on - strand at 1072.994 kb on - strand at 1073.141 kb on - strand at 1073.142 kb on + strand at 1073.142 kb on + strand at 1073.220 kb on - strand, within EX28DRAFT_1062 at 1073.293 kb on + strand, within EX28DRAFT_1062 at 1073.294 kb on - strand, within EX28DRAFT_1062 at 1073.297 kb on - strand, within EX28DRAFT_1062 at 1073.410 kb on + strand, within EX28DRAFT_1062 at 1073.410 kb on + strand, within EX28DRAFT_1062 at 1073.606 kb on - strand, within EX28DRAFT_1062 at 1073.676 kb on + strand, within EX28DRAFT_1062 at 1073.756 kb on - strand, within EX28DRAFT_1062 at 1073.769 kb on - strand, within EX28DRAFT_1062 at 1073.769 kb on - strand, within EX28DRAFT_1062 at 1074.224 kb on + strand at 1074.277 kb on - strand at 1074.406 kb on + strand at 1074.409 kb on - strand at 1074.417 kb on + strand at 1074.564 kb on + strand, within EX28DRAFT_1063 at 1074.717 kb on + strand, within EX28DRAFT_1063 at 1074.718 kb on - strand, within EX28DRAFT_1063 at 1074.763 kb on + strand, within EX28DRAFT_1063 at 1074.914 kb on - strand, within EX28DRAFT_1063 at 1074.914 kb on - strand, within EX28DRAFT_1063 at 1074.916 kb on - strand, within EX28DRAFT_1063 at 1074.916 kb on - strand, within EX28DRAFT_1063 at 1074.948 kb on - strand, within EX28DRAFT_1063
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,072,227 - EX28DRAFT_1061 0.53 -0.0 1,072,353 - EX28DRAFT_1061 0.60 -1.1 1,072,353 - EX28DRAFT_1061 0.60 -0.3 1,072,409 - EX28DRAFT_1061 0.63 -1.4 1,072,409 - EX28DRAFT_1061 0.63 -0.3 1,072,541 + EX28DRAFT_1061 0.71 -1.3 1,072,560 + EX28DRAFT_1061 0.72 -0.6 1,072,566 - EX28DRAFT_1061 0.73 -0.1 1,072,726 + EX28DRAFT_1061 0.82 -0.7 1,072,726 + EX28DRAFT_1061 0.82 +1.4 1,072,727 - EX28DRAFT_1061 0.82 +0.4 1,072,836 - EX28DRAFT_1061 0.89 +0.5 1,072,956 - -0.5 1,072,994 - -0.8 1,073,141 - -0.0 1,073,142 + -0.3 1,073,142 + +0.6 1,073,220 - EX28DRAFT_1062 0.17 +0.5 1,073,293 + EX28DRAFT_1062 0.25 +0.2 1,073,294 - EX28DRAFT_1062 0.25 -0.0 1,073,297 - EX28DRAFT_1062 0.25 +0.9 1,073,410 + EX28DRAFT_1062 0.37 +1.9 1,073,410 + EX28DRAFT_1062 0.37 -1.2 1,073,606 - EX28DRAFT_1062 0.58 -3.1 1,073,676 + EX28DRAFT_1062 0.65 +0.4 1,073,756 - EX28DRAFT_1062 0.74 +0.0 1,073,769 - EX28DRAFT_1062 0.75 +0.6 1,073,769 - EX28DRAFT_1062 0.75 -1.7 1,074,224 + -0.3 1,074,277 - -1.2 1,074,406 + -0.3 1,074,409 - +0.6 1,074,417 + +0.4 1,074,564 + EX28DRAFT_1063 0.12 +0.0 1,074,717 + EX28DRAFT_1063 0.23 -1.3 1,074,718 - EX28DRAFT_1063 0.23 +0.3 1,074,763 + EX28DRAFT_1063 0.27 +1.4 1,074,914 - EX28DRAFT_1063 0.38 +0.5 1,074,914 - EX28DRAFT_1063 0.38 +0.7 1,074,916 - EX28DRAFT_1063 0.38 +0.5 1,074,916 - EX28DRAFT_1063 0.38 -0.8 1,074,948 - EX28DRAFT_1063 0.40 -1.8
Or see this region's nucleotide sequence