Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1057 and EX28DRAFT_1058 are separated by 41 nucleotides EX28DRAFT_1058 and EX28DRAFT_1059 are separated by 37 nucleotides EX28DRAFT_1059 and EX28DRAFT_1060 are separated by 191 nucleotides
EX28DRAFT_1057: EX28DRAFT_1057 - ABC-type amino acid transport/signal transduction systems, periplasmic component/domain, at 1,068,286 to 1,069,173
_1057
EX28DRAFT_1058: EX28DRAFT_1058 - Glutathione S-transferase, at 1,069,215 to 1,069,832
_1058
EX28DRAFT_1059: EX28DRAFT_1059 - Uncharacterized protein, 4-oxalocrotonate tautomerase homolog, at 1,069,870 to 1,070,100
_1059
EX28DRAFT_1060: EX28DRAFT_1060 - lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase, at 1,070,292 to 1,071,293
_1060
Position (kb)
1069
1070
1071 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1068.895 kb on + strand, within EX28DRAFT_1057 at 1068.895 kb on + strand, within EX28DRAFT_1057 at 1068.896 kb on - strand, within EX28DRAFT_1057 at 1068.896 kb on - strand, within EX28DRAFT_1057 at 1068.896 kb on - strand, within EX28DRAFT_1057 at 1068.898 kb on - strand, within EX28DRAFT_1057 at 1068.898 kb on - strand, within EX28DRAFT_1057 at 1068.950 kb on - strand, within EX28DRAFT_1057 at 1069.007 kb on - strand, within EX28DRAFT_1057 at 1069.169 kb on - strand at 1069.169 kb on - strand at 1069.169 kb on - strand at 1069.169 kb on - strand at 1069.171 kb on + strand at 1069.182 kb on + strand at 1069.220 kb on + strand at 1069.538 kb on + strand, within EX28DRAFT_1058 at 1069.539 kb on - strand, within EX28DRAFT_1058 at 1069.542 kb on + strand, within EX28DRAFT_1058 at 1069.543 kb on - strand, within EX28DRAFT_1058 at 1069.543 kb on - strand, within EX28DRAFT_1058 at 1069.624 kb on - strand, within EX28DRAFT_1058 at 1069.695 kb on + strand, within EX28DRAFT_1058 at 1069.695 kb on + strand, within EX28DRAFT_1058 at 1069.780 kb on + strand at 1069.781 kb on - strand at 1069.781 kb on - strand at 1069.781 kb on - strand at 1069.781 kb on - strand at 1069.824 kb on - strand at 1069.869 kb on + strand at 1069.869 kb on + strand at 1069.880 kb on + strand at 1069.880 kb on + strand at 1069.880 kb on + strand at 1069.881 kb on - strand at 1069.881 kb on - strand at 1069.881 kb on - strand at 1069.881 kb on - strand at 1069.881 kb on - strand at 1069.957 kb on - strand, within EX28DRAFT_1059 at 1070.073 kb on - strand, within EX28DRAFT_1059 at 1070.089 kb on + strand at 1070.090 kb on - strand at 1070.098 kb on + strand at 1070.185 kb on + strand at 1070.186 kb on - strand at 1070.275 kb on + strand at 1070.967 kb on + strand, within EX28DRAFT_1060 at 1070.967 kb on + strand, within EX28DRAFT_1060 at 1070.968 kb on - strand, within EX28DRAFT_1060 at 1070.968 kb on - strand, within EX28DRAFT_1060 at 1070.968 kb on - strand, within EX28DRAFT_1060 at 1071.050 kb on + strand, within EX28DRAFT_1060
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,068,895 + EX28DRAFT_1057 0.69 -2.2 1,068,895 + EX28DRAFT_1057 0.69 +0.6 1,068,896 - EX28DRAFT_1057 0.69 +0.1 1,068,896 - EX28DRAFT_1057 0.69 -0.6 1,068,896 - EX28DRAFT_1057 0.69 -0.1 1,068,898 - EX28DRAFT_1057 0.69 +0.2 1,068,898 - EX28DRAFT_1057 0.69 +0.1 1,068,950 - EX28DRAFT_1057 0.75 -0.9 1,069,007 - EX28DRAFT_1057 0.81 +0.3 1,069,169 - -0.1 1,069,169 - -1.4 1,069,169 - -1.5 1,069,169 - -0.4 1,069,171 + +0.5 1,069,182 + -0.2 1,069,220 + +0.6 1,069,538 + EX28DRAFT_1058 0.52 -0.1 1,069,539 - EX28DRAFT_1058 0.52 +1.2 1,069,542 + EX28DRAFT_1058 0.53 -0.1 1,069,543 - EX28DRAFT_1058 0.53 -0.7 1,069,543 - EX28DRAFT_1058 0.53 -0.9 1,069,624 - EX28DRAFT_1058 0.66 +1.1 1,069,695 + EX28DRAFT_1058 0.78 -0.6 1,069,695 + EX28DRAFT_1058 0.78 -1.9 1,069,780 + -1.0 1,069,781 - +0.3 1,069,781 - -0.2 1,069,781 - -0.4 1,069,781 - +0.1 1,069,824 - -0.5 1,069,869 + +0.1 1,069,869 + -2.7 1,069,880 + -1.5 1,069,880 + -1.4 1,069,880 + +0.8 1,069,881 - -1.3 1,069,881 - +0.0 1,069,881 - +0.9 1,069,881 - -2.2 1,069,881 - +0.1 1,069,957 - EX28DRAFT_1059 0.38 -1.7 1,070,073 - EX28DRAFT_1059 0.88 -0.6 1,070,089 + -3.8 1,070,090 - +0.3 1,070,098 + -0.2 1,070,185 + -0.2 1,070,186 - -0.0 1,070,275 + -0.2 1,070,967 + EX28DRAFT_1060 0.67 +0.4 1,070,967 + EX28DRAFT_1060 0.67 -0.6 1,070,968 - EX28DRAFT_1060 0.67 -0.3 1,070,968 - EX28DRAFT_1060 0.67 -0.1 1,070,968 - EX28DRAFT_1060 0.67 -0.1 1,071,050 + EX28DRAFT_1060 0.76 +0.3
Or see this region's nucleotide sequence