Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1003 and EX28DRAFT_1004 are separated by 154 nucleotides EX28DRAFT_1004 and EX28DRAFT_1005 are separated by 95 nucleotides EX28DRAFT_1005 and EX28DRAFT_1006 are separated by 272 nucleotides
EX28DRAFT_1003: EX28DRAFT_1003 - Methyl-accepting chemotaxis protein, at 1,008,950 to 1,010,092
_1003
EX28DRAFT_1004: EX28DRAFT_1004 - Mg2+ and Co2+ transporters, at 1,010,247 to 1,011,230
_1004
EX28DRAFT_1005: EX28DRAFT_1005 - RNA, at 1,011,326 to 1,011,441
_1005
EX28DRAFT_1006: EX28DRAFT_1006 - Superfamily II DNA and RNA helicases, at 1,011,714 to 1,013,087
_1006
Position (kb)
1010
1011
1012 Strain fitness (log2 ratio)
-1
0
1
2 at 1009.330 kb on + strand, within EX28DRAFT_1003 at 1009.520 kb on - strand, within EX28DRAFT_1003 at 1009.520 kb on - strand, within EX28DRAFT_1003 at 1009.562 kb on - strand, within EX28DRAFT_1003 at 1009.597 kb on + strand, within EX28DRAFT_1003 at 1009.678 kb on - strand, within EX28DRAFT_1003 at 1009.678 kb on - strand, within EX28DRAFT_1003 at 1009.748 kb on - strand, within EX28DRAFT_1003 at 1009.927 kb on + strand, within EX28DRAFT_1003 at 1009.968 kb on + strand, within EX28DRAFT_1003 at 1009.968 kb on + strand, within EX28DRAFT_1003 at 1009.969 kb on - strand, within EX28DRAFT_1003 at 1009.969 kb on - strand, within EX28DRAFT_1003 at 1010.182 kb on + strand at 1010.182 kb on + strand at 1010.182 kb on + strand at 1010.182 kb on + strand at 1010.182 kb on + strand at 1010.182 kb on + strand at 1010.183 kb on - strand at 1010.183 kb on - strand at 1010.183 kb on - strand at 1010.183 kb on - strand at 1010.183 kb on - strand at 1010.211 kb on - strand at 1010.230 kb on - strand at 1010.258 kb on + strand at 1010.340 kb on - strand at 1010.374 kb on + strand, within EX28DRAFT_1004 at 1010.385 kb on + strand, within EX28DRAFT_1004 at 1010.574 kb on + strand, within EX28DRAFT_1004 at 1010.846 kb on + strand, within EX28DRAFT_1004 at 1010.847 kb on - strand, within EX28DRAFT_1004 at 1011.049 kb on - strand, within EX28DRAFT_1004 at 1011.051 kb on - strand, within EX28DRAFT_1004 at 1011.175 kb on + strand at 1011.176 kb on - strand at 1011.316 kb on + strand at 1011.316 kb on + strand at 1011.317 kb on - strand at 1011.317 kb on - strand at 1011.367 kb on - strand, within EX28DRAFT_1005 at 1011.374 kb on + strand, within EX28DRAFT_1005 at 1011.375 kb on - strand, within EX28DRAFT_1005 at 1011.399 kb on - strand, within EX28DRAFT_1005 at 1011.710 kb on + strand at 1012.088 kb on + strand, within EX28DRAFT_1006 at 1012.088 kb on + strand, within EX28DRAFT_1006 at 1012.088 kb on + strand, within EX28DRAFT_1006 at 1012.089 kb on - strand, within EX28DRAFT_1006 at 1012.154 kb on - strand, within EX28DRAFT_1006
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,009,330 + EX28DRAFT_1003 0.33 -0.2 1,009,520 - EX28DRAFT_1003 0.50 -0.0 1,009,520 - EX28DRAFT_1003 0.50 -1.3 1,009,562 - EX28DRAFT_1003 0.54 -0.5 1,009,597 + EX28DRAFT_1003 0.57 -0.6 1,009,678 - EX28DRAFT_1003 0.64 +0.7 1,009,678 - EX28DRAFT_1003 0.64 -0.2 1,009,748 - EX28DRAFT_1003 0.70 -0.0 1,009,927 + EX28DRAFT_1003 0.85 +2.0 1,009,968 + EX28DRAFT_1003 0.89 +1.0 1,009,968 + EX28DRAFT_1003 0.89 -1.5 1,009,969 - EX28DRAFT_1003 0.89 +0.4 1,009,969 - EX28DRAFT_1003 0.89 +0.2 1,010,182 + +0.5 1,010,182 + -0.5 1,010,182 + -0.1 1,010,182 + +1.2 1,010,182 + +1.0 1,010,182 + -0.9 1,010,183 - +0.0 1,010,183 - -0.3 1,010,183 - -0.6 1,010,183 - -0.6 1,010,183 - -0.2 1,010,211 - -1.6 1,010,230 - +0.4 1,010,258 + +0.7 1,010,340 - -0.9 1,010,374 + EX28DRAFT_1004 0.13 +0.8 1,010,385 + EX28DRAFT_1004 0.14 +0.3 1,010,574 + EX28DRAFT_1004 0.33 +1.5 1,010,846 + EX28DRAFT_1004 0.61 -0.0 1,010,847 - EX28DRAFT_1004 0.61 +0.5 1,011,049 - EX28DRAFT_1004 0.82 -0.3 1,011,051 - EX28DRAFT_1004 0.82 +0.2 1,011,175 + +0.2 1,011,176 - +0.3 1,011,316 + +1.0 1,011,316 + -0.2 1,011,317 - +0.6 1,011,317 - +2.0 1,011,367 - EX28DRAFT_1005 0.35 -0.7 1,011,374 + EX28DRAFT_1005 0.41 -0.5 1,011,375 - EX28DRAFT_1005 0.42 -0.4 1,011,399 - EX28DRAFT_1005 0.63 -0.3 1,011,710 + +1.0 1,012,088 + EX28DRAFT_1006 0.27 -0.4 1,012,088 + EX28DRAFT_1006 0.27 +0.5 1,012,088 + EX28DRAFT_1006 0.27 -0.5 1,012,089 - EX28DRAFT_1006 0.27 -0.9 1,012,154 - EX28DRAFT_1006 0.32 +0.2
Or see this region's nucleotide sequence