Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0895 and EX28DRAFT_0896 are separated by 99 nucleotides EX28DRAFT_0896 and EX28DRAFT_0897 overlap by 1 nucleotides EX28DRAFT_0897 and EX28DRAFT_0898 overlap by 10 nucleotides EX28DRAFT_0898 and EX28DRAFT_0899 overlap by 17 nucleotides EX28DRAFT_0899 and EX28DRAFT_0900 overlap by 4 nucleotides EX28DRAFT_0900 and EX28DRAFT_0901 overlap by 26 nucleotides
EX28DRAFT_0895: EX28DRAFT_0895 - Phage small terminase subunit, at 903,925 to 904,680
_0895
EX28DRAFT_0896: EX28DRAFT_0896 - Phage head completion protein (GPL), at 904,780 to 905,286
_0896
EX28DRAFT_0897: EX28DRAFT_0897 - P2-like prophage tail protein X, at 905,286 to 905,489
_0897
EX28DRAFT_0898: EX28DRAFT_0898 - holin, HP1 family, at 905,480 to 905,701
_0898
EX28DRAFT_0899: EX28DRAFT_0899 - Phage-related lysozyme (muraminidase), at 905,685 to 906,194
_0899
EX28DRAFT_0900: EX28DRAFT_0900 - phage lysis regulatory protein, LysB family, at 906,191 to 906,616
_0900
EX28DRAFT_0901: EX28DRAFT_0901 - hypothetical protein, at 906,591 to 906,749
_0901
Position (kb) 905
906 Strain fitness (log2 ratio) -2
-1
0
1
2 at 904.508 kb on + strand, within EX28DRAFT_0895 at 904.795 kb on - strand at 904.839 kb on - strand, within EX28DRAFT_0896 at 905.098 kb on + strand, within EX28DRAFT_0896 at 905.137 kb on + strand, within EX28DRAFT_0896 at 905.295 kb on + strand at 905.296 kb on - strand at 905.432 kb on - strand, within EX28DRAFT_0897 at 905.479 kb on + strand at 905.480 kb on - strand at 905.480 kb on - strand at 905.492 kb on - strand at 905.544 kb on - strand, within EX28DRAFT_0898 at 905.580 kb on - strand, within EX28DRAFT_0898 at 905.621 kb on + strand, within EX28DRAFT_0898 at 905.621 kb on + strand, within EX28DRAFT_0898 at 905.621 kb on + strand, within EX28DRAFT_0898 at 905.621 kb on + strand, within EX28DRAFT_0898 at 905.622 kb on - strand, within EX28DRAFT_0898 at 905.642 kb on + strand, within EX28DRAFT_0898 at 905.643 kb on - strand, within EX28DRAFT_0898 at 905.763 kb on - strand, within EX28DRAFT_0899 at 906.115 kb on - strand, within EX28DRAFT_0899 at 906.390 kb on + strand, within EX28DRAFT_0900 at 906.485 kb on + strand, within EX28DRAFT_0900 at 906.558 kb on + strand, within EX28DRAFT_0900 at 906.637 kb on + strand, within EX28DRAFT_0901 at 906.638 kb on - strand, within EX28DRAFT_0901 at 906.638 kb on - strand, within EX28DRAFT_0901
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 904,508 + EX28DRAFT_0895 0.77 +1.1 904,795 - +0.5 904,839 - EX28DRAFT_0896 0.12 +0.3 905,098 + EX28DRAFT_0896 0.63 +1.4 905,137 + EX28DRAFT_0896 0.70 -1.6 905,295 + +0.1 905,296 - +1.2 905,432 - EX28DRAFT_0897 0.72 +0.2 905,479 + +0.7 905,480 - -0.0 905,480 - -0.6 905,492 - +0.3 905,544 - EX28DRAFT_0898 0.29 +0.3 905,580 - EX28DRAFT_0898 0.45 -2.0 905,621 + EX28DRAFT_0898 0.64 +0.5 905,621 + EX28DRAFT_0898 0.64 -0.1 905,621 + EX28DRAFT_0898 0.64 -0.6 905,621 + EX28DRAFT_0898 0.64 -0.8 905,622 - EX28DRAFT_0898 0.64 +1.5 905,642 + EX28DRAFT_0898 0.73 -0.1 905,643 - EX28DRAFT_0898 0.73 +0.4 905,763 - EX28DRAFT_0899 0.15 +0.5 906,115 - EX28DRAFT_0899 0.84 -1.3 906,390 + EX28DRAFT_0900 0.47 -1.0 906,485 + EX28DRAFT_0900 0.69 +0.8 906,558 + EX28DRAFT_0900 0.86 -0.2 906,637 + EX28DRAFT_0901 0.29 -1.2 906,638 - EX28DRAFT_0901 0.30 +2.6 906,638 - EX28DRAFT_0901 0.30 -2.1
Or see this region's nucleotide sequence