Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0441 and EX28DRAFT_0442 overlap by 26 nucleotides EX28DRAFT_0442 and EX28DRAFT_0443 are separated by 487 nucleotides EX28DRAFT_0443 and EX28DRAFT_0444 overlap by 1 nucleotides EX28DRAFT_0444 and EX28DRAFT_0445 overlap by 1 nucleotides
EX28DRAFT_0441: EX28DRAFT_0441 - Acetyltransferases, at 422,753 to 423,208
_0441
EX28DRAFT_0442: EX28DRAFT_0442 - Predicted integral membrane protein, at 423,183 to 423,515
_0442
EX28DRAFT_0443: EX28DRAFT_0443 - Periplasmic protein involved in polysaccharide export, at 424,003 to 425,143
_0443
EX28DRAFT_0444: EX28DRAFT_0444 - Protein-tyrosine-phosphatase, at 425,143 to 425,592
_0444
EX28DRAFT_0445: EX28DRAFT_0445 - capsular exopolysaccharide family, at 425,592 to 427,772
_0445
Position (kb)
424
425
426 Strain fitness (log2 ratio)
-2
-1
0
1 at 423.200 kb on + strand at 423.200 kb on + strand at 423.200 kb on + strand at 423.212 kb on - strand at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.253 kb on + strand, within EX28DRAFT_0442 at 423.254 kb on - strand, within EX28DRAFT_0442 at 423.254 kb on - strand, within EX28DRAFT_0442 at 423.268 kb on + strand, within EX28DRAFT_0442 at 423.268 kb on + strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.269 kb on - strand, within EX28DRAFT_0442 at 423.309 kb on + strand, within EX28DRAFT_0442 at 423.310 kb on - strand, within EX28DRAFT_0442 at 423.310 kb on - strand, within EX28DRAFT_0442 at 423.316 kb on + strand, within EX28DRAFT_0442 at 423.331 kb on + strand, within EX28DRAFT_0442 at 423.412 kb on + strand, within EX28DRAFT_0442 at 423.413 kb on - strand, within EX28DRAFT_0442 at 423.480 kb on + strand, within EX28DRAFT_0442 at 423.481 kb on - strand, within EX28DRAFT_0442 at 423.483 kb on + strand at 423.484 kb on - strand at 423.484 kb on - strand at 423.491 kb on + strand at 423.593 kb on - strand at 423.606 kb on - strand at 423.800 kb on + strand at 423.821 kb on + strand at 423.822 kb on - strand at 423.887 kb on + strand at 424.332 kb on + strand, within EX28DRAFT_0443 at 424.332 kb on + strand, within EX28DRAFT_0443 at 424.371 kb on + strand, within EX28DRAFT_0443 at 424.382 kb on - strand, within EX28DRAFT_0443 at 424.395 kb on + strand, within EX28DRAFT_0443 at 424.519 kb on + strand, within EX28DRAFT_0443 at 424.519 kb on + strand, within EX28DRAFT_0443 at 424.520 kb on - strand, within EX28DRAFT_0443 at 424.520 kb on - strand, within EX28DRAFT_0443 at 424.520 kb on - strand, within EX28DRAFT_0443 at 424.632 kb on + strand, within EX28DRAFT_0443 at 424.680 kb on + strand, within EX28DRAFT_0443 at 424.681 kb on - strand, within EX28DRAFT_0443 at 424.681 kb on - strand, within EX28DRAFT_0443 at 424.801 kb on - strand, within EX28DRAFT_0443 at 424.873 kb on + strand, within EX28DRAFT_0443 at 424.915 kb on + strand, within EX28DRAFT_0443 at 424.915 kb on + strand, within EX28DRAFT_0443 at 424.916 kb on - strand, within EX28DRAFT_0443 at 424.945 kb on + strand, within EX28DRAFT_0443 at 424.949 kb on + strand, within EX28DRAFT_0443 at 424.949 kb on + strand, within EX28DRAFT_0443 at 424.950 kb on - strand, within EX28DRAFT_0443 at 425.012 kb on + strand, within EX28DRAFT_0443 at 425.025 kb on - strand, within EX28DRAFT_0443 at 425.025 kb on - strand, within EX28DRAFT_0443 at 425.120 kb on + strand at 425.141 kb on + strand at 425.142 kb on - strand at 425.178 kb on + strand at 425.238 kb on + strand, within EX28DRAFT_0444 at 425.519 kb on - strand, within EX28DRAFT_0444 at 425.519 kb on - strand, within EX28DRAFT_0444 at 425.519 kb on - strand, within EX28DRAFT_0444 at 425.591 kb on - strand at 425.604 kb on - strand at 425.616 kb on + strand at 425.616 kb on + strand at 425.616 kb on + strand at 425.616 kb on + strand at 425.616 kb on + strand at 425.616 kb on + strand at 425.617 kb on - strand at 425.619 kb on - strand at 425.915 kb on - strand, within EX28DRAFT_0445 at 425.925 kb on + strand, within EX28DRAFT_0445 at 425.925 kb on + strand, within EX28DRAFT_0445 at 425.925 kb on + strand, within EX28DRAFT_0445 at 425.926 kb on - strand, within EX28DRAFT_0445 at 425.926 kb on - strand, within EX28DRAFT_0445 at 425.987 kb on + strand, within EX28DRAFT_0445 at 425.988 kb on - strand, within EX28DRAFT_0445 at 426.001 kb on + strand, within EX28DRAFT_0445 at 426.002 kb on - strand, within EX28DRAFT_0445
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 423,200 + -0.9 423,200 + -0.2 423,200 + -0.1 423,212 - -0.1 423,253 + EX28DRAFT_0442 0.21 +1.3 423,253 + EX28DRAFT_0442 0.21 +0.2 423,253 + EX28DRAFT_0442 0.21 +0.3 423,253 + EX28DRAFT_0442 0.21 -0.3 423,254 - EX28DRAFT_0442 0.21 -1.0 423,254 - EX28DRAFT_0442 0.21 -0.1 423,268 + EX28DRAFT_0442 0.26 +0.2 423,268 + EX28DRAFT_0442 0.26 -0.3 423,269 - EX28DRAFT_0442 0.26 -1.1 423,269 - EX28DRAFT_0442 0.26 -0.2 423,269 - EX28DRAFT_0442 0.26 +0.5 423,269 - EX28DRAFT_0442 0.26 +0.8 423,309 + EX28DRAFT_0442 0.38 -0.3 423,310 - EX28DRAFT_0442 0.38 +0.3 423,310 - EX28DRAFT_0442 0.38 +0.3 423,316 + EX28DRAFT_0442 0.40 +0.0 423,331 + EX28DRAFT_0442 0.44 -0.5 423,412 + EX28DRAFT_0442 0.69 -0.5 423,413 - EX28DRAFT_0442 0.69 -0.3 423,480 + EX28DRAFT_0442 0.89 -1.9 423,481 - EX28DRAFT_0442 0.89 +0.4 423,483 + -0.3 423,484 - +0.7 423,484 - -0.2 423,491 + +1.3 423,593 - +0.6 423,606 - -0.1 423,800 + -0.8 423,821 + -0.8 423,822 - +0.0 423,887 + -0.7 424,332 + EX28DRAFT_0443 0.29 -0.3 424,332 + EX28DRAFT_0443 0.29 +0.2 424,371 + EX28DRAFT_0443 0.32 +0.1 424,382 - EX28DRAFT_0443 0.33 -0.3 424,395 + EX28DRAFT_0443 0.34 -0.2 424,519 + EX28DRAFT_0443 0.45 +0.5 424,519 + EX28DRAFT_0443 0.45 +0.4 424,520 - EX28DRAFT_0443 0.45 -0.5 424,520 - EX28DRAFT_0443 0.45 +0.2 424,520 - EX28DRAFT_0443 0.45 -1.8 424,632 + EX28DRAFT_0443 0.55 -0.5 424,680 + EX28DRAFT_0443 0.59 +0.3 424,681 - EX28DRAFT_0443 0.59 -1.0 424,681 - EX28DRAFT_0443 0.59 +0.1 424,801 - EX28DRAFT_0443 0.70 +0.6 424,873 + EX28DRAFT_0443 0.76 -0.8 424,915 + EX28DRAFT_0443 0.80 +0.4 424,915 + EX28DRAFT_0443 0.80 -0.4 424,916 - EX28DRAFT_0443 0.80 -1.7 424,945 + EX28DRAFT_0443 0.83 -1.0 424,949 + EX28DRAFT_0443 0.83 -0.2 424,949 + EX28DRAFT_0443 0.83 +0.8 424,950 - EX28DRAFT_0443 0.83 -1.1 425,012 + EX28DRAFT_0443 0.88 +0.3 425,025 - EX28DRAFT_0443 0.90 -0.1 425,025 - EX28DRAFT_0443 0.90 +0.5 425,120 + -0.1 425,141 + -0.5 425,142 - -1.0 425,178 + +0.1 425,238 + EX28DRAFT_0444 0.21 -0.7 425,519 - EX28DRAFT_0444 0.84 +1.2 425,519 - EX28DRAFT_0444 0.84 -0.7 425,519 - EX28DRAFT_0444 0.84 -0.3 425,591 - +0.7 425,604 - -0.5 425,616 + -1.0 425,616 + -1.3 425,616 + -1.2 425,616 + -1.7 425,616 + +0.1 425,616 + -0.2 425,617 - +0.1 425,619 - -0.3 425,915 - EX28DRAFT_0445 0.15 -2.1 425,925 + EX28DRAFT_0445 0.15 -0.0 425,925 + EX28DRAFT_0445 0.15 +0.0 425,925 + EX28DRAFT_0445 0.15 +1.4 425,926 - EX28DRAFT_0445 0.15 +0.3 425,926 - EX28DRAFT_0445 0.15 +1.1 425,987 + EX28DRAFT_0445 0.18 +0.7 425,988 - EX28DRAFT_0445 0.18 +0.2 426,001 + EX28DRAFT_0445 0.19 -0.7 426,002 - EX28DRAFT_0445 0.19 +0.6
Or see this region's nucleotide sequence