Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0359 and EX28DRAFT_0360 are separated by 325 nucleotides EX28DRAFT_0360 and EX28DRAFT_0361 are separated by 324 nucleotides
EX28DRAFT_0359: EX28DRAFT_0359 - Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase/Hpt domain, at 327,732 to 330,404
_0359
EX28DRAFT_0360: EX28DRAFT_0360 - RNA, at 330,730 to 330,823
_0360
EX28DRAFT_0361: EX28DRAFT_0361 - Outer membrane protein (porin), at 331,148 to 332,263
_0361
Position (kb)
330
331 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 329.765 kb on + strand, within EX28DRAFT_0359 at 329.771 kb on - strand, within EX28DRAFT_0359 at 329.965 kb on + strand, within EX28DRAFT_0359 at 329.966 kb on - strand, within EX28DRAFT_0359 at 329.968 kb on + strand, within EX28DRAFT_0359 at 329.969 kb on - strand, within EX28DRAFT_0359 at 329.969 kb on - strand, within EX28DRAFT_0359 at 330.014 kb on - strand, within EX28DRAFT_0359 at 330.069 kb on - strand, within EX28DRAFT_0359 at 330.106 kb on + strand, within EX28DRAFT_0359 at 330.107 kb on - strand, within EX28DRAFT_0359 at 330.174 kb on - strand at 330.209 kb on + strand at 330.213 kb on + strand at 330.213 kb on + strand at 330.213 kb on + strand at 330.213 kb on + strand at 330.214 kb on - strand at 330.214 kb on - strand at 330.253 kb on + strand at 330.253 kb on + strand at 330.260 kb on + strand at 330.292 kb on - strand at 330.292 kb on - strand at 330.292 kb on - strand at 330.387 kb on - strand at 330.409 kb on - strand at 330.453 kb on + strand at 330.453 kb on + strand at 330.454 kb on - strand at 330.463 kb on + strand at 330.463 kb on + strand at 330.463 kb on + strand at 330.464 kb on - strand at 330.464 kb on - strand at 330.560 kb on - strand at 330.626 kb on - strand at 330.804 kb on + strand, within EX28DRAFT_0360 at 330.805 kb on - strand, within EX28DRAFT_0360 at 330.805 kb on - strand, within EX28DRAFT_0360 at 330.805 kb on - strand, within EX28DRAFT_0360 at 330.805 kb on - strand, within EX28DRAFT_0360 at 330.829 kb on + strand at 330.963 kb on - strand at 331.119 kb on + strand at 331.121 kb on + strand at 331.121 kb on + strand at 331.129 kb on - strand at 331.129 kb on - strand at 331.130 kb on + strand at 331.130 kb on + strand at 331.130 kb on + strand at 331.131 kb on - strand at 331.131 kb on - strand at 331.131 kb on - strand at 331.131 kb on - strand at 331.145 kb on - strand at 331.156 kb on + strand at 331.162 kb on - strand at 331.162 kb on - strand at 331.162 kb on - strand at 331.242 kb on + strand at 331.243 kb on - strand at 331.274 kb on + strand, within EX28DRAFT_0361 at 331.313 kb on + strand, within EX28DRAFT_0361 at 331.313 kb on + strand, within EX28DRAFT_0361 at 331.313 kb on + strand, within EX28DRAFT_0361 at 331.313 kb on + strand, within EX28DRAFT_0361 at 331.314 kb on - strand, within EX28DRAFT_0361 at 331.487 kb on - strand, within EX28DRAFT_0361 at 331.491 kb on - strand, within EX28DRAFT_0361 at 331.510 kb on - strand, within EX28DRAFT_0361 at 331.554 kb on - strand, within EX28DRAFT_0361 at 331.656 kb on - strand, within EX28DRAFT_0361 at 331.677 kb on + strand, within EX28DRAFT_0361 at 331.748 kb on + strand, within EX28DRAFT_0361 at 331.748 kb on + strand, within EX28DRAFT_0361 at 331.749 kb on - strand, within EX28DRAFT_0361 at 331.749 kb on - strand, within EX28DRAFT_0361
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 329,765 + EX28DRAFT_0359 0.76 -1.4 329,771 - EX28DRAFT_0359 0.76 -0.4 329,965 + EX28DRAFT_0359 0.84 +0.4 329,966 - EX28DRAFT_0359 0.84 +0.4 329,968 + EX28DRAFT_0359 0.84 -0.5 329,969 - EX28DRAFT_0359 0.84 -0.7 329,969 - EX28DRAFT_0359 0.84 +0.3 330,014 - EX28DRAFT_0359 0.85 -0.1 330,069 - EX28DRAFT_0359 0.87 -0.2 330,106 + EX28DRAFT_0359 0.89 +0.8 330,107 - EX28DRAFT_0359 0.89 -0.5 330,174 - -1.7 330,209 + -0.2 330,213 + -0.1 330,213 + -0.3 330,213 + +1.1 330,213 + +0.5 330,214 - +0.0 330,214 - -0.0 330,253 + -0.2 330,253 + -0.6 330,260 + -0.0 330,292 - +0.3 330,292 - +0.0 330,292 - +0.4 330,387 - -0.5 330,409 - +0.3 330,453 + -0.1 330,453 + +0.1 330,454 - +0.3 330,463 + -0.1 330,463 + -0.3 330,463 + -0.4 330,464 - -0.1 330,464 - -0.7 330,560 - -0.3 330,626 - +1.2 330,804 + EX28DRAFT_0360 0.79 -2.0 330,805 - EX28DRAFT_0360 0.80 +0.6 330,805 - EX28DRAFT_0360 0.80 -0.6 330,805 - EX28DRAFT_0360 0.80 -3.0 330,805 - EX28DRAFT_0360 0.80 -2.4 330,829 + -0.1 330,963 - +0.3 331,119 + +1.2 331,121 + -0.0 331,121 + +1.3 331,129 - -0.5 331,129 - -0.4 331,130 + -1.4 331,130 + +0.5 331,130 + -1.0 331,131 - -0.3 331,131 - +0.2 331,131 - -0.3 331,131 - +0.2 331,145 - -2.0 331,156 + +0.5 331,162 - +0.3 331,162 - +0.1 331,162 - -0.7 331,242 + -0.8 331,243 - -1.8 331,274 + EX28DRAFT_0361 0.11 +0.0 331,313 + EX28DRAFT_0361 0.15 -0.2 331,313 + EX28DRAFT_0361 0.15 +1.9 331,313 + EX28DRAFT_0361 0.15 -0.4 331,313 + EX28DRAFT_0361 0.15 -0.7 331,314 - EX28DRAFT_0361 0.15 -0.1 331,487 - EX28DRAFT_0361 0.30 -1.1 331,491 - EX28DRAFT_0361 0.31 -0.2 331,510 - EX28DRAFT_0361 0.32 -0.3 331,554 - EX28DRAFT_0361 0.36 -0.4 331,656 - EX28DRAFT_0361 0.46 -1.8 331,677 + EX28DRAFT_0361 0.47 +0.0 331,748 + EX28DRAFT_0361 0.54 -0.1 331,748 + EX28DRAFT_0361 0.54 -0.1 331,749 - EX28DRAFT_0361 0.54 -0.0 331,749 - EX28DRAFT_0361 0.54 +0.1
Or see this region's nucleotide sequence