Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0082 and EX28DRAFT_0083 overlap by 1 nucleotides EX28DRAFT_0083 and EX28DRAFT_0084 overlap by 4 nucleotides EX28DRAFT_0084 and EX28DRAFT_0085 are separated by 46 nucleotides
EX28DRAFT_0082: EX28DRAFT_0082 - pyridoxine 5'-phosphate synthase, at 22,836 to 23,567
_0082
EX28DRAFT_0083: EX28DRAFT_0083 - holo-[acyl-carrier-protein] synthase, at 23,567 to 23,947
_0083
EX28DRAFT_0084: EX28DRAFT_0084 - 4Fe-4S dicluster domain, at 23,944 to 24,204
_0084
EX28DRAFT_0085: EX28DRAFT_0085 - transcriptional regulator, RpiR family, at 24,251 to 25,099
_0085
Position (kb) 23
24
25 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 23.376 kb on + strand, within EX28DRAFT_0082 at 23.945 kb on + strand at 23.946 kb on - strand at 23.980 kb on - strand, within EX28DRAFT_0084 at 23.987 kb on - strand, within EX28DRAFT_0084 at 24.106 kb on - strand, within EX28DRAFT_0084 at 24.165 kb on + strand, within EX28DRAFT_0084 at 24.165 kb on + strand, within EX28DRAFT_0084 at 24.193 kb on + strand at 24.244 kb on - strand at 24.245 kb on + strand at 24.245 kb on + strand at 24.246 kb on - strand at 24.412 kb on + strand, within EX28DRAFT_0085 at 24.413 kb on - strand, within EX28DRAFT_0085 at 24.413 kb on - strand, within EX28DRAFT_0085 at 24.414 kb on + strand, within EX28DRAFT_0085 at 24.414 kb on + strand, within EX28DRAFT_0085 at 24.416 kb on + strand, within EX28DRAFT_0085 at 24.416 kb on + strand, within EX28DRAFT_0085 at 24.417 kb on - strand, within EX28DRAFT_0085 at 24.417 kb on - strand, within EX28DRAFT_0085 at 24.538 kb on - strand, within EX28DRAFT_0085 at 24.920 kb on - strand, within EX28DRAFT_0085 at 25.089 kb on - strand at 25.100 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 23,376 + EX28DRAFT_0082 0.74 -0.6 23,945 + -0.2 23,946 - -0.4 23,980 - EX28DRAFT_0084 0.14 -1.0 23,987 - EX28DRAFT_0084 0.16 +0.2 24,106 - EX28DRAFT_0084 0.62 -1.2 24,165 + EX28DRAFT_0084 0.85 -1.2 24,165 + EX28DRAFT_0084 0.85 -1.8 24,193 + -0.6 24,244 - -0.0 24,245 + +3.2 24,245 + -1.1 24,246 - +0.4 24,412 + EX28DRAFT_0085 0.19 -0.4 24,413 - EX28DRAFT_0085 0.19 -1.2 24,413 - EX28DRAFT_0085 0.19 -0.9 24,414 + EX28DRAFT_0085 0.19 -1.2 24,414 + EX28DRAFT_0085 0.19 -0.0 24,416 + EX28DRAFT_0085 0.19 -2.6 24,416 + EX28DRAFT_0085 0.19 -0.1 24,417 - EX28DRAFT_0085 0.20 +0.4 24,417 - EX28DRAFT_0085 0.20 +0.2 24,538 - EX28DRAFT_0085 0.34 +0.1 24,920 - EX28DRAFT_0085 0.79 +1.0 25,089 - -0.7 25,100 - -0.3
Or see this region's nucleotide sequence