Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS23330 and AMB_RS08850 are separated by 5 nucleotides AMB_RS08850 and AMB_RS08855 overlap by 4 nucleotides AMB_RS08855 and AMB_RS08860 are separated by 2 nucleotides AMB_RS08860 and AMB_RS24340 are separated by 39 nucleotides AMB_RS24340 and AMB_RS08875 overlap by 4 nucleotides AMB_RS08875 and AMB_RS08880 are separated by 6 nucleotides
AMB_RS23330: AMB_RS23330 - hypothetical protein, at 1,892,802 to 1,893,638
_RS23330
AMB_RS08850: AMB_RS08850 - hypothetical protein, at 1,893,644 to 1,893,922
_RS08850
AMB_RS08855: AMB_RS08855 - hypothetical protein, at 1,893,919 to 1,894,128
_RS08855
AMB_RS08860: AMB_RS08860 - hypothetical protein, at 1,894,131 to 1,894,460
_RS08860
AMB_RS24340: AMB_RS24340 - DNA cytosine methyltransferase, at 1,894,500 to 1,895,732
_RS24340
AMB_RS08875: AMB_RS08875 - regulatory protein GemA, at 1,895,729 to 1,896,361
_RS08875
AMB_RS08880: AMB_RS08880 - hypothetical protein, at 1,896,368 to 1,896,868
_RS08880
Position (kb)
1894
1895
1896 Strain fitness (log2 ratio)
-1
0
1 at 1893.636 kb on + strand at 1893.637 kb on - strand at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.027 kb on + strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.028 kb on - strand, within AMB_RS08855 at 1894.052 kb on - strand, within AMB_RS08855 at 1894.187 kb on + strand, within AMB_RS08860 at 1894.519 kb on + strand at 1894.519 kb on + strand at 1894.519 kb on + strand at 1894.520 kb on - strand at 1894.520 kb on - strand at 1894.867 kb on + strand, within AMB_RS24340 at 1894.867 kb on + strand, within AMB_RS24340 at 1894.867 kb on + strand, within AMB_RS24340 at 1894.867 kb on + strand, within AMB_RS24340 at 1894.867 kb on + strand, within AMB_RS24340 at 1894.867 kb on + strand, within AMB_RS24340 at 1894.868 kb on - strand, within AMB_RS24340 at 1895.354 kb on + strand, within AMB_RS24340 at 1895.354 kb on + strand, within AMB_RS24340 at 1895.355 kb on - strand, within AMB_RS24340
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 1,893,636 + -0.2 1,893,637 - -0.3 1,894,027 + AMB_RS08855 0.51 -0.7 1,894,027 + AMB_RS08855 0.51 +0.6 1,894,027 + AMB_RS08855 0.51 -0.3 1,894,027 + AMB_RS08855 0.51 +0.7 1,894,028 - AMB_RS08855 0.52 +0.3 1,894,028 - AMB_RS08855 0.52 +0.2 1,894,028 - AMB_RS08855 0.52 +0.5 1,894,028 - AMB_RS08855 0.52 -0.1 1,894,052 - AMB_RS08855 0.63 -0.7 1,894,187 + AMB_RS08860 0.17 +0.2 1,894,519 + +0.1 1,894,519 + -0.9 1,894,519 + -0.6 1,894,520 - +0.1 1,894,520 - -0.1 1,894,867 + AMB_RS24340 0.30 -0.8 1,894,867 + AMB_RS24340 0.30 -0.1 1,894,867 + AMB_RS24340 0.30 -0.2 1,894,867 + AMB_RS24340 0.30 -1.2 1,894,867 + AMB_RS24340 0.30 -0.4 1,894,867 + AMB_RS24340 0.30 +0.2 1,894,868 - AMB_RS24340 0.30 -1.5 1,895,354 + AMB_RS24340 0.69 +0.0 1,895,354 + AMB_RS24340 0.69 -0.8 1,895,355 - AMB_RS24340 0.69 -0.0
Or see this region's nucleotide sequence