Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20485 and AMB_RS20490 are separated by 8 nucleotides AMB_RS20490 and AMB_RS20495 are separated by 119 nucleotides AMB_RS20495 and AMB_RS20500 are separated by 163 nucleotides AMB_RS20500 and AMB_RS23640 are separated by 123 nucleotides AMB_RS23640 and AMB_RS24700 overlap by 44 nucleotides AMB_RS24700 and AMB_RS24705 are separated by 83 nucleotides
AMB_RS20485: AMB_RS20485 - hypothetical protein, at 4,450,041 to 4,450,238
_RS20485
AMB_RS20490: AMB_RS20490 - hypothetical protein, at 4,450,247 to 4,450,510
_RS20490
AMB_RS20495: AMB_RS20495 - helix-turn-helix domain-containing protein, at 4,450,630 to 4,450,812
_RS20495
AMB_RS20500: AMB_RS20500 - tRNA-Gly, at 4,450,976 to 4,451,049
_RS20500
AMB_RS23640: AMB_RS23640 - hypothetical protein, at 4,451,173 to 4,454,229
_RS23640
AMB_RS24700: AMB_RS24700 - hypothetical protein, at 4,454,186 to 4,454,935
_RS24700
AMB_RS24705: AMB_RS24705 - hypothetical protein, at 4,455,019 to 4,455,216
_RS24705
Position (kb) 4451
4452
4453
4454
4455 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.475 kb on + strand, within AMB_RS20490 at 4450.476 kb on - strand, within AMB_RS20490 at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.559 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.648 kb on + strand at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.649 kb on - strand, within AMB_RS20495 at 4450.843 kb on + strand at 4450.843 kb on + strand at 4450.844 kb on - strand at 4450.844 kb on - strand at 4450.844 kb on - strand at 4450.884 kb on + strand at 4450.884 kb on + strand at 4450.885 kb on - strand at 4450.893 kb on - strand at 4451.041 kb on + strand, within AMB_RS20500 at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.182 kb on + strand at 4451.253 kb on + strand at 4451.253 kb on + strand at 4451.254 kb on - strand at 4451.254 kb on - strand at 4451.384 kb on + strand at 4451.384 kb on + strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.385 kb on - strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.457 kb on + strand at 4451.458 kb on - strand at 4451.458 kb on - strand at 4451.458 kb on - strand at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.547 kb on + strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.548 kb on - strand, within AMB_RS23640 at 4451.625 kb on + strand, within AMB_RS23640 at 4451.625 kb on + strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.626 kb on - strand, within AMB_RS23640 at 4451.675 kb on + strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.676 kb on - strand, within AMB_RS23640 at 4451.717 kb on + strand, within AMB_RS23640 at 4451.717 kb on + strand, within AMB_RS23640 at 4451.718 kb on - strand, within AMB_RS23640 at 4451.718 kb on - strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.723 kb on + strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.724 kb on - strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.747 kb on + strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.748 kb on - strand, within AMB_RS23640 at 4451.816 kb on + strand, within AMB_RS23640 at 4451.817 kb on - strand, within AMB_RS23640 at 4451.817 kb on - strand, within AMB_RS23640 at 4451.817 kb on - strand, within AMB_RS23640 at 4451.817 kb on - strand, within AMB_RS23640 at 4451.817 kb on - strand, within AMB_RS23640 at 4451.878 kb on - strand, within AMB_RS23640 at 4451.952 kb on + strand, within AMB_RS23640 at 4451.953 kb on - strand, within AMB_RS23640 at 4451.954 kb on + strand, within AMB_RS23640 at 4452.167 kb on + strand, within AMB_RS23640 at 4452.167 kb on + strand, within AMB_RS23640 at 4452.168 kb on - strand, within AMB_RS23640 at 4452.168 kb on - strand, within AMB_RS23640 at 4452.168 kb on - strand, within AMB_RS23640 at 4452.271 kb on - strand, within AMB_RS23640 at 4452.271 kb on - strand, within AMB_RS23640 at 4452.359 kb on + strand, within AMB_RS23640 at 4452.359 kb on + strand, within AMB_RS23640 at 4452.359 kb on + strand, within AMB_RS23640 at 4452.360 kb on - strand, within AMB_RS23640 at 4452.360 kb on - strand, within AMB_RS23640 at 4452.360 kb on - strand, within AMB_RS23640 at 4452.360 kb on - strand, within AMB_RS23640 at 4452.360 kb on - strand, within AMB_RS23640 at 4452.364 kb on + strand, within AMB_RS23640 at 4452.365 kb on - strand, within AMB_RS23640 at 4452.365 kb on - strand, within AMB_RS23640 at 4452.396 kb on + strand, within AMB_RS23640 at 4452.538 kb on + strand, within AMB_RS23640 at 4452.539 kb on - strand, within AMB_RS23640 at 4452.578 kb on + strand, within AMB_RS23640 at 4452.835 kb on - strand, within AMB_RS23640 at 4452.835 kb on - strand, within AMB_RS23640 at 4452.965 kb on + strand, within AMB_RS23640 at 4452.966 kb on - strand, within AMB_RS23640 at 4453.076 kb on + strand, within AMB_RS23640 at 4453.076 kb on + strand, within AMB_RS23640 at 4453.077 kb on - strand, within AMB_RS23640 at 4453.193 kb on + strand, within AMB_RS23640 at 4453.193 kb on + strand, within AMB_RS23640 at 4453.193 kb on + strand, within AMB_RS23640 at 4453.193 kb on + strand, within AMB_RS23640 at 4453.193 kb on + strand, within AMB_RS23640 at 4453.194 kb on - strand, within AMB_RS23640 at 4453.263 kb on + strand, within AMB_RS23640 at 4453.264 kb on - strand, within AMB_RS23640 at 4453.264 kb on - strand, within AMB_RS23640 at 4453.299 kb on + strand, within AMB_RS23640 at 4453.299 kb on + strand, within AMB_RS23640 at 4453.299 kb on + strand, within AMB_RS23640 at 4453.299 kb on + strand, within AMB_RS23640 at 4453.300 kb on - strand, within AMB_RS23640 at 4453.300 kb on - strand, within AMB_RS23640 at 4453.300 kb on - strand, within AMB_RS23640 at 4453.346 kb on + strand, within AMB_RS23640 at 4453.347 kb on - strand, within AMB_RS23640 at 4453.347 kb on - strand, within AMB_RS23640 at 4453.347 kb on - strand, within AMB_RS23640 at 4453.530 kb on + strand, within AMB_RS23640 at 4453.531 kb on - strand, within AMB_RS23640 at 4453.531 kb on - strand, within AMB_RS23640 at 4453.556 kb on + strand, within AMB_RS23640 at 4453.556 kb on + strand, within AMB_RS23640 at 4453.557 kb on - strand, within AMB_RS23640 at 4453.602 kb on + strand, within AMB_RS23640 at 4453.602 kb on + strand, within AMB_RS23640 at 4453.602 kb on + strand, within AMB_RS23640 at 4453.603 kb on - strand, within AMB_RS23640 at 4453.803 kb on + strand, within AMB_RS23640 at 4453.863 kb on + strand, within AMB_RS23640 at 4453.863 kb on + strand, within AMB_RS23640 at 4453.864 kb on - strand, within AMB_RS23640 at 4453.951 kb on + strand at 4453.952 kb on - strand at 4453.956 kb on - strand at 4453.956 kb on - strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.078 kb on + strand at 4454.079 kb on - strand at 4454.079 kb on - strand at 4454.079 kb on - strand at 4454.079 kb on - strand at 4454.102 kb on + strand at 4454.248 kb on + strand at 4454.248 kb on + strand at 4454.249 kb on - strand at 4454.355 kb on + strand, within AMB_RS24700 at 4454.356 kb on - strand, within AMB_RS24700 at 4454.379 kb on + strand, within AMB_RS24700 at 4454.379 kb on + strand, within AMB_RS24700 at 4454.379 kb on + strand, within AMB_RS24700 at 4454.379 kb on + strand, within AMB_RS24700 at 4454.379 kb on + strand, within AMB_RS24700 at 4454.380 kb on - strand, within AMB_RS24700 at 4454.457 kb on + strand, within AMB_RS24700 at 4454.933 kb on + strand at 4454.933 kb on + strand at 4454.934 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 4,450,475 + AMB_RS20490 0.86 +0.6 4,450,475 + AMB_RS20490 0.86 -0.7 4,450,476 - AMB_RS20490 0.87 +0.2 4,450,559 + +0.0 4,450,559 + -1.0 4,450,559 + -0.8 4,450,648 + -0.5 4,450,648 + -0.8 4,450,648 + -0.4 4,450,648 + -0.9 4,450,648 + -0.4 4,450,648 + +2.1 4,450,648 + -1.1 4,450,648 + +0.3 4,450,649 - AMB_RS20495 0.10 -0.0 4,450,649 - AMB_RS20495 0.10 -0.9 4,450,649 - AMB_RS20495 0.10 +0.4 4,450,649 - AMB_RS20495 0.10 +2.1 4,450,843 + -0.5 4,450,843 + -0.0 4,450,844 - -0.0 4,450,844 - -0.9 4,450,844 - +0.0 4,450,884 + +0.1 4,450,884 + -0.3 4,450,885 - +0.4 4,450,893 - +0.2 4,451,041 + AMB_RS20500 0.88 -0.8 4,451,182 + +0.2 4,451,182 + +0.2 4,451,182 + -0.2 4,451,182 + -0.0 4,451,253 + -0.2 4,451,253 + -1.1 4,451,254 - +0.2 4,451,254 - -1.3 4,451,384 + -0.7 4,451,384 + -0.5 4,451,385 - -0.7 4,451,385 - -0.5 4,451,385 - -0.1 4,451,385 - -0.7 4,451,457 + -0.2 4,451,457 + -0.1 4,451,457 + +0.7 4,451,457 + -0.6 4,451,458 - -0.3 4,451,458 - +0.7 4,451,458 - +0.7 4,451,547 + AMB_RS23640 0.12 -1.7 4,451,547 + AMB_RS23640 0.12 -0.3 4,451,547 + AMB_RS23640 0.12 -0.3 4,451,547 + AMB_RS23640 0.12 -0.1 4,451,548 - AMB_RS23640 0.12 +0.1 4,451,548 - AMB_RS23640 0.12 +0.0 4,451,548 - AMB_RS23640 0.12 -1.0 4,451,548 - AMB_RS23640 0.12 -0.1 4,451,548 - AMB_RS23640 0.12 -0.2 4,451,625 + AMB_RS23640 0.15 -0.7 4,451,625 + AMB_RS23640 0.15 -0.4 4,451,626 - AMB_RS23640 0.15 -0.0 4,451,626 - AMB_RS23640 0.15 +0.5 4,451,626 - AMB_RS23640 0.15 +0.2 4,451,675 + AMB_RS23640 0.16 -0.2 4,451,676 - AMB_RS23640 0.16 +0.3 4,451,676 - AMB_RS23640 0.16 +0.5 4,451,676 - AMB_RS23640 0.16 -0.1 4,451,676 - AMB_RS23640 0.16 -0.2 4,451,717 + AMB_RS23640 0.18 -0.1 4,451,717 + AMB_RS23640 0.18 -0.8 4,451,718 - AMB_RS23640 0.18 +1.0 4,451,718 - AMB_RS23640 0.18 +0.0 4,451,723 + AMB_RS23640 0.18 -0.7 4,451,723 + AMB_RS23640 0.18 +0.9 4,451,723 + AMB_RS23640 0.18 +0.2 4,451,723 + AMB_RS23640 0.18 +1.3 4,451,723 + AMB_RS23640 0.18 -0.4 4,451,724 - AMB_RS23640 0.18 -1.3 4,451,724 - AMB_RS23640 0.18 -1.9 4,451,724 - AMB_RS23640 0.18 -0.2 4,451,724 - AMB_RS23640 0.18 +0.2 4,451,724 - AMB_RS23640 0.18 -0.2 4,451,724 - AMB_RS23640 0.18 -0.4 4,451,724 - AMB_RS23640 0.18 -2.0 4,451,747 + AMB_RS23640 0.19 -0.1 4,451,747 + AMB_RS23640 0.19 -0.8 4,451,747 + AMB_RS23640 0.19 +0.7 4,451,748 - AMB_RS23640 0.19 -1.0 4,451,748 - AMB_RS23640 0.19 -1.2 4,451,748 - AMB_RS23640 0.19 -0.9 4,451,748 - AMB_RS23640 0.19 -0.4 4,451,816 + AMB_RS23640 0.21 -0.2 4,451,817 - AMB_RS23640 0.21 -1.1 4,451,817 - AMB_RS23640 0.21 +0.4 4,451,817 - AMB_RS23640 0.21 -1.0 4,451,817 - AMB_RS23640 0.21 -0.3 4,451,817 - AMB_RS23640 0.21 +1.3 4,451,878 - AMB_RS23640 0.23 +0.1 4,451,952 + AMB_RS23640 0.25 +0.1 4,451,953 - AMB_RS23640 0.26 +0.3 4,451,954 + AMB_RS23640 0.26 -1.0 4,452,167 + AMB_RS23640 0.33 +0.3 4,452,167 + AMB_RS23640 0.33 -0.8 4,452,168 - AMB_RS23640 0.33 -0.5 4,452,168 - AMB_RS23640 0.33 -0.7 4,452,168 - AMB_RS23640 0.33 +0.7 4,452,271 - AMB_RS23640 0.36 -0.3 4,452,271 - AMB_RS23640 0.36 +0.4 4,452,359 + AMB_RS23640 0.39 -1.5 4,452,359 + AMB_RS23640 0.39 -1.9 4,452,359 + AMB_RS23640 0.39 +0.3 4,452,360 - AMB_RS23640 0.39 -0.2 4,452,360 - AMB_RS23640 0.39 -0.3 4,452,360 - AMB_RS23640 0.39 -0.2 4,452,360 - AMB_RS23640 0.39 +0.6 4,452,360 - AMB_RS23640 0.39 +0.1 4,452,364 + AMB_RS23640 0.39 +0.3 4,452,365 - AMB_RS23640 0.39 -1.1 4,452,365 - AMB_RS23640 0.39 +0.6 4,452,396 + AMB_RS23640 0.40 +0.0 4,452,538 + AMB_RS23640 0.45 -1.6 4,452,539 - AMB_RS23640 0.45 -0.1 4,452,578 + AMB_RS23640 0.46 +1.1 4,452,835 - AMB_RS23640 0.54 -0.0 4,452,835 - AMB_RS23640 0.54 +0.6 4,452,965 + AMB_RS23640 0.59 -0.7 4,452,966 - AMB_RS23640 0.59 -1.3 4,453,076 + AMB_RS23640 0.62 -0.1 4,453,076 + AMB_RS23640 0.62 +0.3 4,453,077 - AMB_RS23640 0.62 -0.3 4,453,193 + AMB_RS23640 0.66 -0.6 4,453,193 + AMB_RS23640 0.66 -0.4 4,453,193 + AMB_RS23640 0.66 -0.1 4,453,193 + AMB_RS23640 0.66 -0.3 4,453,193 + AMB_RS23640 0.66 +0.4 4,453,194 - AMB_RS23640 0.66 -0.0 4,453,263 + AMB_RS23640 0.68 +0.3 4,453,264 - AMB_RS23640 0.68 +0.8 4,453,264 - AMB_RS23640 0.68 +0.4 4,453,299 + AMB_RS23640 0.70 +1.3 4,453,299 + AMB_RS23640 0.70 -0.3 4,453,299 + AMB_RS23640 0.70 -0.6 4,453,299 + AMB_RS23640 0.70 -3.5 4,453,300 - AMB_RS23640 0.70 +0.2 4,453,300 - AMB_RS23640 0.70 +0.4 4,453,300 - AMB_RS23640 0.70 -0.4 4,453,346 + AMB_RS23640 0.71 -1.2 4,453,347 - AMB_RS23640 0.71 -0.3 4,453,347 - AMB_RS23640 0.71 -0.5 4,453,347 - AMB_RS23640 0.71 +0.1 4,453,530 + AMB_RS23640 0.77 +0.6 4,453,531 - AMB_RS23640 0.77 -1.1 4,453,531 - AMB_RS23640 0.77 -1.7 4,453,556 + AMB_RS23640 0.78 +0.7 4,453,556 + AMB_RS23640 0.78 -1.1 4,453,557 - AMB_RS23640 0.78 -2.3 4,453,602 + AMB_RS23640 0.79 -0.2 4,453,602 + AMB_RS23640 0.79 +0.8 4,453,602 + AMB_RS23640 0.79 -0.0 4,453,603 - AMB_RS23640 0.79 +0.1 4,453,803 + AMB_RS23640 0.86 +0.3 4,453,863 + AMB_RS23640 0.88 -0.2 4,453,863 + AMB_RS23640 0.88 -0.3 4,453,864 - AMB_RS23640 0.88 +0.1 4,453,951 + -0.4 4,453,952 - +0.7 4,453,956 - -0.5 4,453,956 - +0.0 4,454,078 + -0.2 4,454,078 + -0.2 4,454,078 + +0.3 4,454,078 + +1.7 4,454,078 + +0.1 4,454,078 + +0.3 4,454,078 + -1.3 4,454,078 + -0.0 4,454,078 + -0.8 4,454,079 - -0.3 4,454,079 - +0.5 4,454,079 - -0.9 4,454,079 - +0.9 4,454,102 + +0.3 4,454,248 + -0.3 4,454,248 + +1.3 4,454,249 - +0.3 4,454,355 + AMB_RS24700 0.23 -0.1 4,454,356 - AMB_RS24700 0.23 -1.0 4,454,379 + AMB_RS24700 0.26 -0.7 4,454,379 + AMB_RS24700 0.26 +0.0 4,454,379 + AMB_RS24700 0.26 +0.3 4,454,379 + AMB_RS24700 0.26 -0.5 4,454,379 + AMB_RS24700 0.26 -0.4 4,454,380 - AMB_RS24700 0.26 +0.5 4,454,457 + AMB_RS24700 0.36 +0.3 4,454,933 + -0.2 4,454,933 + +0.4 4,454,934 - -0.4
Or see this region's nucleotide sequence