Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18065 and AMB_RS24645 are separated by 89 nucleotides AMB_RS24645 and AMB_RS23565 overlap by 350 nucleotides AMB_RS23565 and AMB_RS18080 are separated by 115 nucleotides
AMB_RS18065: AMB_RS18065 - response regulator, at 3,924,254 to 3,924,619
_RS18065
AMB_RS24645: AMB_RS24645 - DUF423 domain-containing protein, at 3,924,709 to 3,925,506
_RS24645
AMB_RS23565: AMB_RS23565 - hypothetical protein, at 3,925,157 to 3,925,975
_RS23565
AMB_RS18080: AMB_RS18080 - formyltetrahydrofolate deformylase, at 3,926,091 to 3,926,951
_RS18080
Position (kb)
3925
3926 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3924.314 kb on - strand, within AMB_RS18065 at 3924.314 kb on - strand, within AMB_RS18065 at 3924.316 kb on - strand, within AMB_RS18065 at 3924.316 kb on - strand, within AMB_RS18065 at 3924.613 kb on + strand at 3924.614 kb on - strand at 3924.614 kb on - strand at 3924.614 kb on - strand at 3924.614 kb on - strand at 3924.629 kb on - strand at 3924.629 kb on - strand at 3924.629 kb on - strand at 3924.629 kb on - strand at 3925.112 kb on - strand, within AMB_RS24645 at 3925.118 kb on + strand, within AMB_RS24645 at 3925.118 kb on + strand, within AMB_RS24645 at 3925.119 kb on - strand, within AMB_RS24645 at 3925.119 kb on - strand, within AMB_RS24645 at 3925.119 kb on - strand, within AMB_RS24645 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.616 kb on + strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.617 kb on - strand, within AMB_RS23565 at 3925.920 kb on - strand at 3926.281 kb on + strand, within AMB_RS18080 at 3926.281 kb on + strand, within AMB_RS18080 at 3926.281 kb on + strand, within AMB_RS18080 at 3926.281 kb on + strand, within AMB_RS18080 at 3926.281 kb on + strand, within AMB_RS18080 at 3926.281 kb on + strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.282 kb on - strand, within AMB_RS18080 at 3926.332 kb on + strand, within AMB_RS18080 at 3926.432 kb on - strand, within AMB_RS18080 at 3926.432 kb on - strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.527 kb on + strand, within AMB_RS18080 at 3926.533 kb on + strand, within AMB_RS18080 at 3926.534 kb on - strand, within AMB_RS18080 at 3926.623 kb on + strand, within AMB_RS18080 at 3926.624 kb on - strand, within AMB_RS18080 at 3926.624 kb on - strand, within AMB_RS18080 at 3926.793 kb on + strand, within AMB_RS18080 at 3926.793 kb on + strand, within AMB_RS18080 at 3926.794 kb on - strand, within AMB_RS18080 at 3926.794 kb on - strand, within AMB_RS18080 at 3926.794 kb on - strand, within AMB_RS18080 at 3926.884 kb on - strand at 3926.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 3,924,314 - AMB_RS18065 0.16 -0.1 3,924,314 - AMB_RS18065 0.16 -0.3 3,924,316 - AMB_RS18065 0.17 -0.1 3,924,316 - AMB_RS18065 0.17 -0.2 3,924,613 + +0.8 3,924,614 - +1.2 3,924,614 - +0.9 3,924,614 - +0.2 3,924,614 - -3.5 3,924,629 - -0.2 3,924,629 - -0.2 3,924,629 - -1.0 3,924,629 - -2.9 3,925,112 - AMB_RS24645 0.51 -0.8 3,925,118 + AMB_RS24645 0.51 -1.4 3,925,118 + AMB_RS24645 0.51 +0.2 3,925,119 - AMB_RS24645 0.51 -0.5 3,925,119 - AMB_RS24645 0.51 -0.0 3,925,119 - AMB_RS24645 0.51 -0.8 3,925,616 + AMB_RS23565 0.56 +0.0 3,925,616 + AMB_RS23565 0.56 -0.9 3,925,616 + AMB_RS23565 0.56 -0.7 3,925,616 + AMB_RS23565 0.56 -1.2 3,925,616 + AMB_RS23565 0.56 +0.3 3,925,616 + AMB_RS23565 0.56 +0.2 3,925,617 - AMB_RS23565 0.56 +0.2 3,925,617 - AMB_RS23565 0.56 +0.1 3,925,617 - AMB_RS23565 0.56 -0.4 3,925,617 - AMB_RS23565 0.56 -0.3 3,925,617 - AMB_RS23565 0.56 +0.3 3,925,617 - AMB_RS23565 0.56 -0.8 3,925,617 - AMB_RS23565 0.56 -1.9 3,925,920 - -0.2 3,926,281 + AMB_RS18080 0.22 -0.1 3,926,281 + AMB_RS18080 0.22 -0.3 3,926,281 + AMB_RS18080 0.22 -0.8 3,926,281 + AMB_RS18080 0.22 -0.2 3,926,281 + AMB_RS18080 0.22 -0.2 3,926,281 + AMB_RS18080 0.22 -0.1 3,926,282 - AMB_RS18080 0.22 +0.3 3,926,282 - AMB_RS18080 0.22 +0.1 3,926,282 - AMB_RS18080 0.22 -0.1 3,926,282 - AMB_RS18080 0.22 -0.1 3,926,282 - AMB_RS18080 0.22 -0.8 3,926,282 - AMB_RS18080 0.22 +0.1 3,926,332 + AMB_RS18080 0.28 -0.9 3,926,432 - AMB_RS18080 0.40 -0.8 3,926,432 - AMB_RS18080 0.40 +0.1 3,926,527 + AMB_RS18080 0.51 +0.3 3,926,527 + AMB_RS18080 0.51 -0.0 3,926,527 + AMB_RS18080 0.51 +0.2 3,926,527 + AMB_RS18080 0.51 -0.3 3,926,527 + AMB_RS18080 0.51 -0.4 3,926,527 + AMB_RS18080 0.51 +0.4 3,926,533 + AMB_RS18080 0.51 -1.9 3,926,534 - AMB_RS18080 0.51 +0.0 3,926,623 + AMB_RS18080 0.62 -0.9 3,926,624 - AMB_RS18080 0.62 -1.9 3,926,624 - AMB_RS18080 0.62 -0.5 3,926,793 + AMB_RS18080 0.82 +0.1 3,926,793 + AMB_RS18080 0.82 +0.3 3,926,794 - AMB_RS18080 0.82 +0.2 3,926,794 - AMB_RS18080 0.82 +2.0 3,926,794 - AMB_RS18080 0.82 +0.5 3,926,884 - +0.3 3,926,907 - -0.3
Or see this region's nucleotide sequence