Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS07565 and AMB_RS07570 overlap by 1 nucleotides AMB_RS07570 and AMB_RS07575 overlap by 4 nucleotides AMB_RS07575 and AMB_RS23300 overlap by 4 nucleotides AMB_RS23300 and AMB_RS07585 are separated by 119 nucleotides
AMB_RS07565: AMB_RS07565 - single-stranded DNA-binding protein, at 1,636,204 to 1,636,785
_RS07565
AMB_RS07570: AMB_RS07570 - hypothetical protein, at 1,636,785 to 1,637,390
_RS07570
AMB_RS07575: AMB_RS07575 - hypothetical protein, at 1,637,387 to 1,637,692
_RS07575
AMB_RS23300: AMB_RS23300 - transcriptional regulator, at 1,637,689 to 1,639,923
_RS23300
AMB_RS07585: AMB_RS07585 - hypothetical protein, at 1,640,043 to 1,640,663
_RS07585
Position (kb)
1637
1638
1639
1640 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1636.786 kb on + strand at 1636.787 kb on - strand at 1637.008 kb on + strand, within AMB_RS07570 at 1637.008 kb on + strand, within AMB_RS07570 at 1637.008 kb on + strand, within AMB_RS07570 at 1637.643 kb on + strand, within AMB_RS07575 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.068 kb on + strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.069 kb on - strand, within AMB_RS23300 at 1638.114 kb on + strand, within AMB_RS23300 at 1638.115 kb on - strand, within AMB_RS23300 at 1638.299 kb on + strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300 at 1638.300 kb on - strand, within AMB_RS23300 at 1638.485 kb on + strand, within AMB_RS23300 at 1638.485 kb on + strand, within AMB_RS23300 at 1638.485 kb on + strand, within AMB_RS23300 at 1638.486 kb on - strand, within AMB_RS23300 at 1638.672 kb on - strand, within AMB_RS23300 at 1638.672 kb on - strand, within AMB_RS23300 at 1638.672 kb on - strand, within AMB_RS23300 at 1638.672 kb on - strand, within AMB_RS23300 at 1638.813 kb on - strand, within AMB_RS23300 at 1638.869 kb on + strand, within AMB_RS23300 at 1638.869 kb on + strand, within AMB_RS23300 at 1638.870 kb on - strand, within AMB_RS23300 at 1638.870 kb on - strand, within AMB_RS23300 at 1638.911 kb on + strand, within AMB_RS23300 at 1638.912 kb on - strand, within AMB_RS23300 at 1638.912 kb on - strand, within AMB_RS23300 at 1638.974 kb on - strand, within AMB_RS23300 at 1639.037 kb on + strand, within AMB_RS23300 at 1639.091 kb on + strand, within AMB_RS23300 at 1639.091 kb on + strand, within AMB_RS23300 at 1639.132 kb on + strand, within AMB_RS23300 at 1639.374 kb on + strand, within AMB_RS23300 at 1639.374 kb on + strand, within AMB_RS23300 at 1639.375 kb on - strand, within AMB_RS23300 at 1639.375 kb on - strand, within AMB_RS23300 at 1639.375 kb on - strand, within AMB_RS23300 at 1639.375 kb on - strand, within AMB_RS23300 at 1639.462 kb on - strand, within AMB_RS23300 at 1639.580 kb on + strand, within AMB_RS23300 at 1639.793 kb on + strand at 1639.794 kb on - strand at 1639.794 kb on - strand at 1639.880 kb on + strand at 1639.881 kb on - strand at 1640.428 kb on + strand, within AMB_RS07585 at 1640.428 kb on + strand, within AMB_RS07585 at 1640.428 kb on + strand, within AMB_RS07585 at 1640.429 kb on - strand, within AMB_RS07585 at 1640.429 kb on - strand, within AMB_RS07585
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 1,636,786 + -0.3 1,636,787 - -2.4 1,637,008 + AMB_RS07570 0.37 +0.0 1,637,008 + AMB_RS07570 0.37 -1.0 1,637,008 + AMB_RS07570 0.37 +0.0 1,637,643 + AMB_RS07575 0.84 +0.5 1,638,068 + AMB_RS23300 0.17 -0.3 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 +0.2 1,638,068 + AMB_RS23300 0.17 +1.3 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 +0.2 1,638,068 + AMB_RS23300 0.17 +0.4 1,638,068 + AMB_RS23300 0.17 -0.2 1,638,068 + AMB_RS23300 0.17 +0.1 1,638,068 + AMB_RS23300 0.17 +0.4 1,638,068 + AMB_RS23300 0.17 -0.1 1,638,068 + AMB_RS23300 0.17 -0.2 1,638,069 - AMB_RS23300 0.17 -0.2 1,638,069 - AMB_RS23300 0.17 +0.1 1,638,069 - AMB_RS23300 0.17 -1.4 1,638,114 + AMB_RS23300 0.19 -0.5 1,638,115 - AMB_RS23300 0.19 -1.0 1,638,299 + AMB_RS23300 0.27 -0.3 1,638,300 - AMB_RS23300 0.27 -0.4 1,638,300 - AMB_RS23300 0.27 +0.4 1,638,300 - AMB_RS23300 0.27 -0.5 1,638,485 + AMB_RS23300 0.36 -0.6 1,638,485 + AMB_RS23300 0.36 +0.2 1,638,485 + AMB_RS23300 0.36 -1.9 1,638,486 - AMB_RS23300 0.36 +1.9 1,638,672 - AMB_RS23300 0.44 -0.4 1,638,672 - AMB_RS23300 0.44 +0.6 1,638,672 - AMB_RS23300 0.44 -0.2 1,638,672 - AMB_RS23300 0.44 -1.2 1,638,813 - AMB_RS23300 0.50 +0.5 1,638,869 + AMB_RS23300 0.53 -1.1 1,638,869 + AMB_RS23300 0.53 -0.3 1,638,870 - AMB_RS23300 0.53 -1.3 1,638,870 - AMB_RS23300 0.53 +0.5 1,638,911 + AMB_RS23300 0.55 -0.4 1,638,912 - AMB_RS23300 0.55 -1.8 1,638,912 - AMB_RS23300 0.55 +0.2 1,638,974 - AMB_RS23300 0.57 -0.5 1,639,037 + AMB_RS23300 0.60 +0.8 1,639,091 + AMB_RS23300 0.63 +0.8 1,639,091 + AMB_RS23300 0.63 +0.3 1,639,132 + AMB_RS23300 0.65 +0.5 1,639,374 + AMB_RS23300 0.75 -0.9 1,639,374 + AMB_RS23300 0.75 -1.6 1,639,375 - AMB_RS23300 0.75 -0.8 1,639,375 - AMB_RS23300 0.75 +0.5 1,639,375 - AMB_RS23300 0.75 +0.7 1,639,375 - AMB_RS23300 0.75 +0.1 1,639,462 - AMB_RS23300 0.79 +0.5 1,639,580 + AMB_RS23300 0.85 +0.4 1,639,793 + -0.2 1,639,794 - -0.6 1,639,794 - +0.0 1,639,880 + -0.1 1,639,881 - -0.2 1,640,428 + AMB_RS07585 0.62 -0.9 1,640,428 + AMB_RS07585 0.62 +0.4 1,640,428 + AMB_RS07585 0.62 -0.3 1,640,429 - AMB_RS07585 0.62 -0.4 1,640,429 - AMB_RS07585 0.62 +0.2
Or see this region's nucleotide sequence