Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS21510 and AMB_RS21515 are separated by 16 nucleotides AMB_RS21515 and AMB_RS21520 are separated by 111 nucleotides AMB_RS21520 and AMB_RS21525 are separated by 281 nucleotides
AMB_RS21510: AMB_RS21510 - hypothetical protein, at 4,665,728 to 4,666,711
_RS21510
AMB_RS21515: AMB_RS21515 - hypothetical protein, at 4,666,728 to 4,667,312
_RS21515
AMB_RS21520: AMB_RS21520 - tRNA-Arg, at 4,667,424 to 4,667,500
_RS21520
AMB_RS21525: AMB_RS21525 - hypothetical protein, at 4,667,782 to 4,668,885
_RS21525
Position (kb)
4667
4668 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4666.556 kb on + strand, within AMB_RS21510 at 4666.557 kb on - strand, within AMB_RS21510 at 4666.557 kb on - strand, within AMB_RS21510 at 4666.584 kb on - strand, within AMB_RS21510 at 4666.584 kb on - strand, within AMB_RS21510 at 4666.584 kb on - strand, within AMB_RS21510 at 4666.665 kb on - strand at 4666.688 kb on + strand at 4666.688 kb on + strand at 4666.688 kb on + strand at 4666.689 kb on - strand at 4666.689 kb on - strand at 4666.689 kb on - strand at 4666.729 kb on + strand at 4666.729 kb on + strand at 4666.729 kb on + strand at 4666.729 kb on + strand at 4666.730 kb on - strand at 4666.730 kb on - strand at 4666.730 kb on - strand at 4666.730 kb on - strand at 4666.735 kb on + strand at 4666.736 kb on - strand at 4666.736 kb on - strand at 4666.750 kb on + strand at 4666.750 kb on + strand at 4666.751 kb on - strand at 4666.821 kb on + strand, within AMB_RS21515 at 4666.821 kb on + strand, within AMB_RS21515 at 4666.821 kb on + strand, within AMB_RS21515 at 4666.821 kb on + strand, within AMB_RS21515 at 4666.821 kb on + strand, within AMB_RS21515 at 4666.822 kb on - strand, within AMB_RS21515 at 4666.822 kb on - strand, within AMB_RS21515 at 4666.822 kb on - strand, within AMB_RS21515 at 4666.822 kb on - strand, within AMB_RS21515 at 4666.960 kb on + strand, within AMB_RS21515 at 4666.961 kb on - strand, within AMB_RS21515 at 4667.019 kb on - strand, within AMB_RS21515 at 4667.025 kb on - strand, within AMB_RS21515 at 4667.025 kb on - strand, within AMB_RS21515 at 4667.025 kb on - strand, within AMB_RS21515 at 4667.025 kb on - strand, within AMB_RS21515 at 4667.027 kb on - strand, within AMB_RS21515 at 4667.062 kb on + strand, within AMB_RS21515 at 4667.062 kb on + strand, within AMB_RS21515 at 4667.062 kb on + strand, within AMB_RS21515 at 4667.063 kb on - strand, within AMB_RS21515 at 4667.063 kb on - strand, within AMB_RS21515 at 4667.063 kb on - strand, within AMB_RS21515 at 4667.063 kb on - strand, within AMB_RS21515 at 4667.093 kb on + strand, within AMB_RS21515 at 4667.093 kb on + strand, within AMB_RS21515 at 4667.107 kb on + strand, within AMB_RS21515 at 4667.108 kb on - strand, within AMB_RS21515 at 4667.312 kb on + strand at 4667.312 kb on + strand at 4667.313 kb on - strand at 4667.313 kb on - strand at 4667.352 kb on + strand at 4667.352 kb on + strand at 4667.353 kb on - strand at 4667.632 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 4,666,556 + AMB_RS21510 0.84 +1.1 4,666,557 - AMB_RS21510 0.84 +0.8 4,666,557 - AMB_RS21510 0.84 -0.5 4,666,584 - AMB_RS21510 0.87 +0.4 4,666,584 - AMB_RS21510 0.87 +0.4 4,666,584 - AMB_RS21510 0.87 -0.4 4,666,665 - +0.0 4,666,688 + +0.6 4,666,688 + -1.2 4,666,688 + +0.1 4,666,689 - -0.2 4,666,689 - -0.1 4,666,689 - -0.9 4,666,729 + +0.1 4,666,729 + +0.6 4,666,729 + -0.1 4,666,729 + +0.1 4,666,730 - -1.1 4,666,730 - +1.1 4,666,730 - +0.1 4,666,730 - -2.3 4,666,735 + -0.0 4,666,736 - +0.7 4,666,736 - +0.3 4,666,750 + -0.5 4,666,750 + -0.2 4,666,751 - -1.1 4,666,821 + AMB_RS21515 0.16 +0.0 4,666,821 + AMB_RS21515 0.16 -0.3 4,666,821 + AMB_RS21515 0.16 +0.3 4,666,821 + AMB_RS21515 0.16 +0.1 4,666,821 + AMB_RS21515 0.16 -0.1 4,666,822 - AMB_RS21515 0.16 +0.2 4,666,822 - AMB_RS21515 0.16 +1.8 4,666,822 - AMB_RS21515 0.16 -1.3 4,666,822 - AMB_RS21515 0.16 -0.0 4,666,960 + AMB_RS21515 0.40 -0.9 4,666,961 - AMB_RS21515 0.40 -0.1 4,667,019 - AMB_RS21515 0.50 +0.5 4,667,025 - AMB_RS21515 0.51 -0.1 4,667,025 - AMB_RS21515 0.51 -0.2 4,667,025 - AMB_RS21515 0.51 -0.4 4,667,025 - AMB_RS21515 0.51 +0.6 4,667,027 - AMB_RS21515 0.51 -1.0 4,667,062 + AMB_RS21515 0.57 +1.5 4,667,062 + AMB_RS21515 0.57 -0.5 4,667,062 + AMB_RS21515 0.57 -0.0 4,667,063 - AMB_RS21515 0.57 -1.7 4,667,063 - AMB_RS21515 0.57 -0.4 4,667,063 - AMB_RS21515 0.57 -0.4 4,667,063 - AMB_RS21515 0.57 -0.1 4,667,093 + AMB_RS21515 0.62 -0.3 4,667,093 + AMB_RS21515 0.62 -0.1 4,667,107 + AMB_RS21515 0.65 -0.7 4,667,108 - AMB_RS21515 0.65 +0.1 4,667,312 + +0.3 4,667,312 + -1.3 4,667,313 - -0.0 4,667,313 - -1.7 4,667,352 + -0.9 4,667,352 + +0.1 4,667,353 - -0.7 4,667,632 + -0.1
Or see this region's nucleotide sequence