Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS19535 and AMB_RS19540 are separated by 546 nucleotides AMB_RS19540 and AMB_RS19545 are separated by 63 nucleotides
AMB_RS19535: AMB_RS19535 - DNA-binding response regulator, at 4,263,981 to 4,264,646
_RS19535
AMB_RS19540: AMB_RS19540 - hypothetical protein, at 4,265,193 to 4,266,776
_RS19540
AMB_RS19545: AMB_RS19545 - elongation factor 4, at 4,266,840 to 4,268,642
_RS19545
Position (kb)
4265
4266
4267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4264.244 kb on + strand, within AMB_RS19535 at 4264.244 kb on + strand, within AMB_RS19535 at 4264.244 kb on + strand, within AMB_RS19535 at 4264.245 kb on - strand, within AMB_RS19535 at 4264.245 kb on - strand, within AMB_RS19535 at 4264.245 kb on - strand, within AMB_RS19535 at 4264.245 kb on - strand, within AMB_RS19535 at 4264.245 kb on - strand, within AMB_RS19535 at 4264.246 kb on + strand, within AMB_RS19535 at 4264.246 kb on + strand, within AMB_RS19535 at 4264.246 kb on + strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.247 kb on - strand, within AMB_RS19535 at 4264.315 kb on + strand, within AMB_RS19535 at 4264.315 kb on + strand, within AMB_RS19535 at 4264.316 kb on - strand, within AMB_RS19535 at 4264.316 kb on - strand, within AMB_RS19535 at 4264.602 kb on + strand at 4264.602 kb on + strand at 4264.602 kb on + strand at 4264.602 kb on + strand at 4264.602 kb on + strand at 4264.602 kb on + strand at 4264.603 kb on - strand at 4264.603 kb on - strand at 4264.603 kb on - strand at 4264.677 kb on + strand at 4264.677 kb on + strand at 4264.678 kb on - strand at 4264.725 kb on + strand at 4264.789 kb on + strand at 4264.790 kb on - strand at 4264.815 kb on + strand at 4264.816 kb on - strand at 4264.987 kb on + strand at 4264.987 kb on + strand at 4264.987 kb on + strand at 4264.988 kb on - strand at 4264.988 kb on - strand at 4264.988 kb on - strand at 4264.988 kb on - strand at 4265.047 kb on - strand at 4265.182 kb on + strand at 4265.182 kb on + strand at 4265.182 kb on + strand at 4265.182 kb on + strand at 4265.183 kb on - strand at 4265.183 kb on - strand at 4265.584 kb on - strand, within AMB_RS19540 at 4265.817 kb on + strand, within AMB_RS19540 at 4266.054 kb on + strand, within AMB_RS19540 at 4266.054 kb on + strand, within AMB_RS19540 at 4266.122 kb on + strand, within AMB_RS19540 at 4266.122 kb on + strand, within AMB_RS19540 at 4266.282 kb on + strand, within AMB_RS19540 at 4266.282 kb on + strand, within AMB_RS19540 at 4266.283 kb on - strand, within AMB_RS19540 at 4266.283 kb on - strand, within AMB_RS19540 at 4266.360 kb on + strand, within AMB_RS19540 at 4266.360 kb on + strand, within AMB_RS19540 at 4266.360 kb on + strand, within AMB_RS19540 at 4266.361 kb on - strand, within AMB_RS19540 at 4266.361 kb on - strand, within AMB_RS19540 at 4266.429 kb on + strand, within AMB_RS19540 at 4266.429 kb on + strand, within AMB_RS19540 at 4266.430 kb on - strand, within AMB_RS19540 at 4266.774 kb on + strand at 4266.774 kb on + strand at 4266.774 kb on + strand at 4266.774 kb on + strand at 4266.774 kb on + strand at 4266.774 kb on + strand at 4266.775 kb on - strand at 4266.775 kb on - strand at 4266.775 kb on - strand at 4266.775 kb on - strand at 4266.775 kb on - strand at 4266.801 kb on + strand at 4266.801 kb on + strand at 4266.801 kb on + strand at 4266.801 kb on + strand at 4266.801 kb on + strand at 4266.801 kb on + strand at 4266.802 kb on - strand at 4267.198 kb on - strand, within AMB_RS19545 at 4267.563 kb on + strand, within AMB_RS19545
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 4,264,244 + AMB_RS19535 0.39 -1.0 4,264,244 + AMB_RS19535 0.39 -0.0 4,264,244 + AMB_RS19535 0.39 -0.1 4,264,245 - AMB_RS19535 0.40 -0.2 4,264,245 - AMB_RS19535 0.40 -0.6 4,264,245 - AMB_RS19535 0.40 -1.0 4,264,245 - AMB_RS19535 0.40 +1.2 4,264,245 - AMB_RS19535 0.40 -1.0 4,264,246 + AMB_RS19535 0.40 -0.2 4,264,246 + AMB_RS19535 0.40 +0.4 4,264,246 + AMB_RS19535 0.40 -0.3 4,264,247 - AMB_RS19535 0.40 +0.2 4,264,247 - AMB_RS19535 0.40 -0.3 4,264,247 - AMB_RS19535 0.40 -0.6 4,264,247 - AMB_RS19535 0.40 -0.2 4,264,247 - AMB_RS19535 0.40 +0.6 4,264,247 - AMB_RS19535 0.40 -0.3 4,264,247 - AMB_RS19535 0.40 +1.8 4,264,315 + AMB_RS19535 0.50 -0.1 4,264,315 + AMB_RS19535 0.50 -1.0 4,264,316 - AMB_RS19535 0.50 +0.2 4,264,316 - AMB_RS19535 0.50 +0.1 4,264,602 + -0.5 4,264,602 + -0.2 4,264,602 + -0.6 4,264,602 + -0.4 4,264,602 + +0.2 4,264,602 + -0.8 4,264,603 - +0.1 4,264,603 - -1.3 4,264,603 - -0.3 4,264,677 + -0.7 4,264,677 + -1.2 4,264,678 - -1.0 4,264,725 + -2.3 4,264,789 + +0.2 4,264,790 - +0.1 4,264,815 + -0.5 4,264,816 - +0.2 4,264,987 + -3.2 4,264,987 + -1.8 4,264,987 + -0.8 4,264,988 - -0.6 4,264,988 - +1.0 4,264,988 - -2.6 4,264,988 - -0.4 4,265,047 - -1.0 4,265,182 + -0.1 4,265,182 + +0.8 4,265,182 + -1.3 4,265,182 + -1.9 4,265,183 - -2.0 4,265,183 - -0.8 4,265,584 - AMB_RS19540 0.25 +0.2 4,265,817 + AMB_RS19540 0.39 -0.3 4,266,054 + AMB_RS19540 0.54 -0.5 4,266,054 + AMB_RS19540 0.54 -0.4 4,266,122 + AMB_RS19540 0.59 +0.1 4,266,122 + AMB_RS19540 0.59 -2.1 4,266,282 + AMB_RS19540 0.69 -0.5 4,266,282 + AMB_RS19540 0.69 -2.2 4,266,283 - AMB_RS19540 0.69 +0.1 4,266,283 - AMB_RS19540 0.69 -0.3 4,266,360 + AMB_RS19540 0.74 -0.9 4,266,360 + AMB_RS19540 0.74 -0.7 4,266,360 + AMB_RS19540 0.74 -0.0 4,266,361 - AMB_RS19540 0.74 -0.0 4,266,361 - AMB_RS19540 0.74 +0.6 4,266,429 + AMB_RS19540 0.78 -2.1 4,266,429 + AMB_RS19540 0.78 -0.7 4,266,430 - AMB_RS19540 0.78 -0.9 4,266,774 + -0.8 4,266,774 + +0.2 4,266,774 + +2.7 4,266,774 + -1.3 4,266,774 + +0.4 4,266,774 + -0.2 4,266,775 - -0.2 4,266,775 - +0.3 4,266,775 - -0.4 4,266,775 - +0.1 4,266,775 - -0.2 4,266,801 + -0.2 4,266,801 + -0.5 4,266,801 + +0.2 4,266,801 + -1.3 4,266,801 + +0.7 4,266,801 + -0.2 4,266,802 - -1.0 4,267,198 - AMB_RS19545 0.20 -0.8 4,267,563 + AMB_RS19545 0.40 -1.9
Or see this region's nucleotide sequence