Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18105 and AMB_RS18110 are separated by 5 nucleotides AMB_RS18110 and AMB_RS18115 are separated by 145 nucleotides AMB_RS18115 and AMB_RS18120 are separated by 125 nucleotides AMB_RS18120 and AMB_RS18125 are separated by 67 nucleotides AMB_RS18125 and AMB_RS18130 are separated by 149 nucleotides
AMB_RS18105: AMB_RS18105 - cAMP-binding protein, at 3,929,986 to 3,930,684
_RS18105
AMB_RS18110: AMB_RS18110 - cAMP-binding protein, at 3,930,690 to 3,931,388
_RS18110
AMB_RS18115: AMB_RS18115 - glutamate racemase, at 3,931,534 to 3,932,355
_RS18115
AMB_RS18120: AMB_RS18120 - hypothetical protein, at 3,932,481 to 3,932,678
_RS18120
AMB_RS18125: AMB_RS18125 - hypothetical protein, at 3,932,746 to 3,932,955
_RS18125
AMB_RS18130: AMB_RS18130 - KR domain-containing protein, at 3,933,105 to 3,933,866
_RS18130
Position (kb)
3931
3932
3933 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3930.576 kb on - strand, within AMB_RS18105 at 3931.102 kb on + strand, within AMB_RS18110 at 3931.102 kb on + strand, within AMB_RS18110 at 3931.222 kb on + strand, within AMB_RS18110 at 3931.223 kb on - strand, within AMB_RS18110 at 3931.223 kb on - strand, within AMB_RS18110 at 3931.223 kb on - strand, within AMB_RS18110 at 3931.401 kb on - strand at 3931.401 kb on - strand at 3931.401 kb on - strand at 3931.401 kb on - strand at 3931.430 kb on + strand at 3931.430 kb on + strand at 3931.431 kb on - strand at 3931.460 kb on + strand at 3931.461 kb on - strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.496 kb on + strand at 3931.500 kb on + strand at 3932.354 kb on - strand at 3932.530 kb on + strand, within AMB_RS18120 at 3932.530 kb on + strand, within AMB_RS18120 at 3932.530 kb on + strand, within AMB_RS18120 at 3932.530 kb on + strand, within AMB_RS18120 at 3932.531 kb on - strand, within AMB_RS18120 at 3932.531 kb on - strand, within AMB_RS18120 at 3932.531 kb on - strand, within AMB_RS18120 at 3932.531 kb on - strand, within AMB_RS18120 at 3932.687 kb on + strand at 3932.687 kb on + strand at 3932.687 kb on + strand at 3932.687 kb on + strand at 3932.688 kb on - strand at 3932.688 kb on - strand at 3932.688 kb on - strand at 3932.725 kb on - strand at 3932.725 kb on - strand at 3932.762 kb on + strand at 3932.763 kb on - strand at 3932.934 kb on - strand, within AMB_RS18125 at 3932.961 kb on + strand at 3932.962 kb on - strand at 3932.988 kb on + strand at 3932.988 kb on + strand at 3932.988 kb on + strand at 3932.989 kb on - strand at 3932.989 kb on - strand at 3932.992 kb on + strand at 3933.042 kb on + strand at 3933.066 kb on + strand at 3933.067 kb on - strand at 3933.293 kb on + strand, within AMB_RS18130 at 3933.293 kb on + strand, within AMB_RS18130 at 3933.293 kb on + strand, within AMB_RS18130 at 3933.293 kb on + strand, within AMB_RS18130 at 3933.293 kb on + strand, within AMB_RS18130 at 3933.293 kb on + strand, within AMB_RS18130 at 3933.294 kb on - strand, within AMB_RS18130 at 3933.294 kb on - strand, within AMB_RS18130 at 3933.294 kb on - strand, within AMB_RS18130 at 3933.294 kb on - strand, within AMB_RS18130 at 3933.294 kb on - strand, within AMB_RS18130 at 3933.295 kb on + strand, within AMB_RS18130 at 3933.295 kb on + strand, within AMB_RS18130 at 3933.295 kb on + strand, within AMB_RS18130 at 3933.295 kb on + strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130 at 3933.296 kb on - strand, within AMB_RS18130
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 3,930,576 - AMB_RS18105 0.84 +1.0 3,931,102 + AMB_RS18110 0.59 +0.0 3,931,102 + AMB_RS18110 0.59 -1.8 3,931,222 + AMB_RS18110 0.76 -0.8 3,931,223 - AMB_RS18110 0.76 -1.7 3,931,223 - AMB_RS18110 0.76 -1.4 3,931,223 - AMB_RS18110 0.76 -0.8 3,931,401 - +0.8 3,931,401 - -2.4 3,931,401 - -0.2 3,931,401 - +0.1 3,931,430 + -0.1 3,931,430 + -0.4 3,931,431 - -0.8 3,931,460 + -1.9 3,931,461 - -0.3 3,931,496 + +0.7 3,931,496 + +0.6 3,931,496 + -1.1 3,931,496 + -2.1 3,931,496 + -0.9 3,931,496 + -0.9 3,931,496 + -0.1 3,931,496 + +1.3 3,931,496 + -0.5 3,931,496 + -0.1 3,931,500 + +0.0 3,932,354 - +0.7 3,932,530 + AMB_RS18120 0.25 +1.6 3,932,530 + AMB_RS18120 0.25 +0.1 3,932,530 + AMB_RS18120 0.25 -0.2 3,932,530 + AMB_RS18120 0.25 -0.4 3,932,531 - AMB_RS18120 0.25 -1.8 3,932,531 - AMB_RS18120 0.25 -0.4 3,932,531 - AMB_RS18120 0.25 +0.4 3,932,531 - AMB_RS18120 0.25 -0.0 3,932,687 + -0.2 3,932,687 + +0.2 3,932,687 + -0.4 3,932,687 + +0.0 3,932,688 - -2.5 3,932,688 - -0.3 3,932,688 - +0.4 3,932,725 - +0.1 3,932,725 - -0.2 3,932,762 + -3.8 3,932,763 - -1.4 3,932,934 - AMB_RS18125 0.90 +0.1 3,932,961 + -0.5 3,932,962 - +0.7 3,932,988 + -0.2 3,932,988 + -0.9 3,932,988 + -1.5 3,932,989 - -0.6 3,932,989 - +0.3 3,932,992 + +0.6 3,933,042 + -1.3 3,933,066 + -0.4 3,933,067 - +1.2 3,933,293 + AMB_RS18130 0.25 +0.1 3,933,293 + AMB_RS18130 0.25 +0.2 3,933,293 + AMB_RS18130 0.25 -1.0 3,933,293 + AMB_RS18130 0.25 -0.0 3,933,293 + AMB_RS18130 0.25 -0.5 3,933,293 + AMB_RS18130 0.25 -0.7 3,933,294 - AMB_RS18130 0.25 -0.5 3,933,294 - AMB_RS18130 0.25 +1.2 3,933,294 - AMB_RS18130 0.25 -0.1 3,933,294 - AMB_RS18130 0.25 -1.4 3,933,294 - AMB_RS18130 0.25 -0.1 3,933,295 + AMB_RS18130 0.25 -0.3 3,933,295 + AMB_RS18130 0.25 -0.2 3,933,295 + AMB_RS18130 0.25 +0.2 3,933,295 + AMB_RS18130 0.25 +0.3 3,933,296 - AMB_RS18130 0.25 -0.1 3,933,296 - AMB_RS18130 0.25 -0.2 3,933,296 - AMB_RS18130 0.25 -0.3 3,933,296 - AMB_RS18130 0.25 -0.4 3,933,296 - AMB_RS18130 0.25 -0.6 3,933,296 - AMB_RS18130 0.25 -0.3
Or see this region's nucleotide sequence