Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15560 and AMB_RS15565 are separated by 133 nucleotides AMB_RS15565 and AMB_RS15570 are separated by 88 nucleotides AMB_RS15570 and AMB_RS15575 overlap by 4 nucleotides
AMB_RS15560: AMB_RS15560 - hemerythrin, at 3,339,267 to 3,339,710
_RS15560
AMB_RS15565: AMB_RS15565 - hypothetical protein, at 3,339,844 to 3,340,224
_RS15565
AMB_RS15570: AMB_RS15570 - signal transduction protein, at 3,340,313 to 3,341,461
_RS15570
AMB_RS15575: AMB_RS15575 - diguanylate cyclase, at 3,341,458 to 3,342,363
_RS15575
Position (kb)
3340
3341
3342 Strain fitness (log2 ratio)
-1
0
1
2 at 3339.346 kb on + strand, within AMB_RS15560 at 3339.697 kb on + strand at 3339.697 kb on + strand at 3339.697 kb on + strand at 3339.697 kb on + strand at 3339.697 kb on + strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.698 kb on - strand at 3339.735 kb on + strand at 3339.735 kb on + strand at 3339.736 kb on - strand at 3339.736 kb on - strand at 3339.826 kb on - strand at 3339.853 kb on + strand at 3340.272 kb on - strand at 3340.283 kb on + strand at 3340.284 kb on - strand at 3340.284 kb on - strand at 3340.379 kb on + strand at 3340.379 kb on + strand at 3340.379 kb on + strand at 3340.380 kb on - strand at 3340.380 kb on - strand at 3340.380 kb on - strand at 3340.806 kb on - strand, within AMB_RS15570 at 3341.070 kb on + strand, within AMB_RS15570 at 3341.482 kb on - strand at 3341.482 kb on - strand at 3341.582 kb on - strand, within AMB_RS15575 at 3342.027 kb on + strand, within AMB_RS15575 at 3342.088 kb on + strand, within AMB_RS15575 at 3342.100 kb on + strand, within AMB_RS15575 at 3342.106 kb on + strand, within AMB_RS15575 at 3342.316 kb on + strand at 3342.317 kb on - strand at 3342.423 kb on - strand at 3342.423 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 3,339,346 + AMB_RS15560 0.18 -0.8 3,339,697 + -1.4 3,339,697 + -0.5 3,339,697 + -0.2 3,339,697 + -0.2 3,339,697 + +0.5 3,339,698 - +0.1 3,339,698 - +0.1 3,339,698 - -0.0 3,339,698 - +0.2 3,339,698 - -0.3 3,339,698 - -0.3 3,339,698 - +0.5 3,339,735 + -0.8 3,339,735 + -1.0 3,339,736 - +0.2 3,339,736 - -0.9 3,339,826 - -0.2 3,339,853 + +0.7 3,340,272 - -0.2 3,340,283 + -1.1 3,340,284 - -0.3 3,340,284 - -0.7 3,340,379 + +0.1 3,340,379 + -0.2 3,340,379 + +0.1 3,340,380 - +2.3 3,340,380 - -0.1 3,340,380 - -0.7 3,340,806 - AMB_RS15570 0.43 -0.2 3,341,070 + AMB_RS15570 0.66 -0.6 3,341,482 - -0.4 3,341,482 - -0.2 3,341,582 - AMB_RS15575 0.14 +0.1 3,342,027 + AMB_RS15575 0.63 -1.2 3,342,088 + AMB_RS15575 0.70 -0.5 3,342,100 + AMB_RS15575 0.71 +0.2 3,342,106 + AMB_RS15575 0.72 -1.4 3,342,316 + -0.9 3,342,317 - +0.5 3,342,423 - -0.4 3,342,423 - -0.1
Or see this region's nucleotide sequence