Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS15470 and AMB_RS15475 overlap by 11 nucleotides AMB_RS15475 and AMB_RS15480 overlap by 4 nucleotides AMB_RS15480 and AMB_RS15485 overlap by 4 nucleotides
AMB_RS15470: AMB_RS15470 - oxidoreductase, at 3,322,859 to 3,323,854
_RS15470
AMB_RS15475: AMB_RS15475 - bile acid:sodium symporter family protein, at 3,323,844 to 3,324,704
_RS15475
AMB_RS15480: AMB_RS15480 - 1,4-dihydroxy-2-naphthoate octaprenyltransferase, at 3,324,701 to 3,325,644
_RS15480
AMB_RS15485: AMB_RS15485 - bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE, at 3,325,641 to 3,326,372
_RS15485
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3322.973 kb on + strand, within AMB_RS15470 at 3322.973 kb on + strand, within AMB_RS15470 at 3322.973 kb on + strand, within AMB_RS15470 at 3322.989 kb on - strand, within AMB_RS15470 at 3323.036 kb on + strand, within AMB_RS15470 at 3323.037 kb on - strand, within AMB_RS15470 at 3323.159 kb on + strand, within AMB_RS15470 at 3323.159 kb on + strand, within AMB_RS15470 at 3323.160 kb on - strand, within AMB_RS15470 at 3323.426 kb on + strand, within AMB_RS15470 at 3323.427 kb on - strand, within AMB_RS15470 at 3324.189 kb on - strand, within AMB_RS15475 at 3324.916 kb on - strand, within AMB_RS15480 at 3324.996 kb on - strand, within AMB_RS15480 at 3324.999 kb on + strand, within AMB_RS15480 at 3324.999 kb on + strand, within AMB_RS15480 at 3325.085 kb on + strand, within AMB_RS15480 at 3325.085 kb on + strand, within AMB_RS15480 at 3325.085 kb on + strand, within AMB_RS15480 at 3325.086 kb on - strand, within AMB_RS15480 at 3325.086 kb on - strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.088 kb on + strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.089 kb on - strand, within AMB_RS15480 at 3325.149 kb on + strand, within AMB_RS15480 at 3325.149 kb on + strand, within AMB_RS15480 at 3325.149 kb on + strand, within AMB_RS15480 at 3325.149 kb on + strand, within AMB_RS15480 at 3325.149 kb on + strand, within AMB_RS15480 at 3325.150 kb on - strand, within AMB_RS15480 at 3325.150 kb on - strand, within AMB_RS15480 at 3325.150 kb on - strand, within AMB_RS15480 at 3325.150 kb on - strand, within AMB_RS15480 at 3325.152 kb on - strand, within AMB_RS15480 at 3325.178 kb on + strand, within AMB_RS15480 at 3325.247 kb on + strand, within AMB_RS15480 at 3325.247 kb on + strand, within AMB_RS15480 at 3325.247 kb on + strand, within AMB_RS15480 at 3325.394 kb on + strand, within AMB_RS15480
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 3,322,973 + AMB_RS15470 0.11 -0.4 3,322,973 + AMB_RS15470 0.11 +1.2 3,322,973 + AMB_RS15470 0.11 -1.6 3,322,989 - AMB_RS15470 0.13 -0.2 3,323,036 + AMB_RS15470 0.18 +3.8 3,323,037 - AMB_RS15470 0.18 -1.1 3,323,159 + AMB_RS15470 0.30 -0.5 3,323,159 + AMB_RS15470 0.30 +0.4 3,323,160 - AMB_RS15470 0.30 +0.1 3,323,426 + AMB_RS15470 0.57 +0.4 3,323,427 - AMB_RS15470 0.57 +0.6 3,324,189 - AMB_RS15475 0.40 +0.8 3,324,916 - AMB_RS15480 0.23 -0.9 3,324,996 - AMB_RS15480 0.31 -0.3 3,324,999 + AMB_RS15480 0.32 -0.1 3,324,999 + AMB_RS15480 0.32 -1.8 3,325,085 + AMB_RS15480 0.41 -0.1 3,325,085 + AMB_RS15480 0.41 -1.3 3,325,085 + AMB_RS15480 0.41 +0.2 3,325,086 - AMB_RS15480 0.41 -0.4 3,325,086 - AMB_RS15480 0.41 -0.1 3,325,088 + AMB_RS15480 0.41 -0.4 3,325,088 + AMB_RS15480 0.41 +0.7 3,325,088 + AMB_RS15480 0.41 +0.5 3,325,088 + AMB_RS15480 0.41 +0.0 3,325,088 + AMB_RS15480 0.41 +0.8 3,325,088 + AMB_RS15480 0.41 -0.3 3,325,088 + AMB_RS15480 0.41 -0.3 3,325,089 - AMB_RS15480 0.41 -0.2 3,325,089 - AMB_RS15480 0.41 +0.5 3,325,089 - AMB_RS15480 0.41 -1.8 3,325,089 - AMB_RS15480 0.41 -0.9 3,325,089 - AMB_RS15480 0.41 -0.4 3,325,089 - AMB_RS15480 0.41 -1.5 3,325,149 + AMB_RS15480 0.47 -0.8 3,325,149 + AMB_RS15480 0.47 -0.3 3,325,149 + AMB_RS15480 0.47 -1.4 3,325,149 + AMB_RS15480 0.47 -0.6 3,325,149 + AMB_RS15480 0.47 +1.4 3,325,150 - AMB_RS15480 0.48 -0.1 3,325,150 - AMB_RS15480 0.48 +0.1 3,325,150 - AMB_RS15480 0.48 -0.9 3,325,150 - AMB_RS15480 0.48 -0.3 3,325,152 - AMB_RS15480 0.48 -0.2 3,325,178 + AMB_RS15480 0.51 +1.4 3,325,247 + AMB_RS15480 0.58 -0.6 3,325,247 + AMB_RS15480 0.58 -0.7 3,325,247 + AMB_RS15480 0.58 +0.6 3,325,394 + AMB_RS15480 0.73 -0.6
Or see this region's nucleotide sequence