Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13100 and AMB_RS13105 overlap by 8 nucleotides AMB_RS13105 and AMB_RS13110 overlap by 8 nucleotides AMB_RS13110 and AMB_RS13115 overlap by 11 nucleotides AMB_RS13115 and AMB_RS13120 are separated by 121 nucleotides
AMB_RS13100: AMB_RS13100 - branched-chain amino acid ABC transporter permease, at 2,790,480 to 2,791,442
_RS13100
AMB_RS13105: AMB_RS13105 - ABC transporter ATP-binding protein, at 2,791,435 to 2,792,148
_RS13105
AMB_RS13110: AMB_RS13110 - ABC transporter ATP-binding protein, at 2,792,141 to 2,792,860
_RS13110
AMB_RS13115: AMB_RS13115 - PaaI family thioesterase, at 2,792,850 to 2,793,281
_RS13115
AMB_RS13120: AMB_RS13120 - nitronate monooxygenase, at 2,793,403 to 2,794,389
_RS13120
Position (kb)
2792
2793 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2791.158 kb on + strand, within AMB_RS13100 at 2791.159 kb on - strand, within AMB_RS13100 at 2791.239 kb on + strand, within AMB_RS13100 at 2791.281 kb on + strand, within AMB_RS13100 at 2791.281 kb on + strand, within AMB_RS13100 at 2792.125 kb on + strand at 2792.125 kb on + strand at 2792.125 kb on + strand at 2792.125 kb on + strand at 2792.126 kb on - strand at 2792.126 kb on - strand at 2792.171 kb on + strand at 2792.318 kb on + strand, within AMB_RS13110 at 2792.319 kb on - strand, within AMB_RS13110 at 2792.493 kb on - strand, within AMB_RS13110 at 2792.493 kb on - strand, within AMB_RS13110 at 2792.493 kb on - strand, within AMB_RS13110 at 2792.753 kb on + strand, within AMB_RS13110 at 2792.754 kb on - strand, within AMB_RS13110 at 2792.828 kb on + strand at 2792.828 kb on + strand at 2792.829 kb on - strand at 2792.829 kb on - strand at 2793.230 kb on + strand, within AMB_RS13115 at 2793.230 kb on + strand, within AMB_RS13115 at 2793.230 kb on + strand, within AMB_RS13115 at 2793.283 kb on + strand at 2793.283 kb on + strand at 2793.283 kb on + strand at 2793.283 kb on + strand at 2793.283 kb on + strand at 2793.284 kb on - strand at 2793.284 kb on - strand at 2793.284 kb on - strand at 2793.284 kb on - strand at 2793.284 kb on - strand at 2793.286 kb on + strand at 2793.286 kb on + strand at 2793.287 kb on - strand at 2793.496 kb on + strand at 2793.496 kb on + strand at 2793.497 kb on - strand at 2793.550 kb on + strand, within AMB_RS13120 at 2793.551 kb on - strand, within AMB_RS13120 at 2793.739 kb on + strand, within AMB_RS13120 at 2793.740 kb on - strand, within AMB_RS13120 at 2793.740 kb on - strand, within AMB_RS13120 at 2793.799 kb on + strand, within AMB_RS13120 at 2793.800 kb on - strand, within AMB_RS13120 at 2793.800 kb on - strand, within AMB_RS13120 at 2793.800 kb on - strand, within AMB_RS13120
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 2,791,158 + AMB_RS13100 0.70 +1.0 2,791,159 - AMB_RS13100 0.71 -0.4 2,791,239 + AMB_RS13100 0.79 -1.2 2,791,281 + AMB_RS13100 0.83 +0.1 2,791,281 + AMB_RS13100 0.83 +1.1 2,792,125 + +0.2 2,792,125 + -0.1 2,792,125 + +0.5 2,792,125 + +0.4 2,792,126 - +0.9 2,792,126 - -0.6 2,792,171 + -0.5 2,792,318 + AMB_RS13110 0.25 +0.2 2,792,319 - AMB_RS13110 0.25 -2.1 2,792,493 - AMB_RS13110 0.49 +2.3 2,792,493 - AMB_RS13110 0.49 -0.0 2,792,493 - AMB_RS13110 0.49 -0.9 2,792,753 + AMB_RS13110 0.85 +0.2 2,792,754 - AMB_RS13110 0.85 -0.3 2,792,828 + -0.4 2,792,828 + -0.4 2,792,829 - -0.1 2,792,829 - -0.2 2,793,230 + AMB_RS13115 0.88 -0.4 2,793,230 + AMB_RS13115 0.88 +0.9 2,793,230 + AMB_RS13115 0.88 -1.3 2,793,283 + +1.5 2,793,283 + -1.8 2,793,283 + -2.0 2,793,283 + -0.2 2,793,283 + -0.8 2,793,284 - +0.4 2,793,284 - +0.2 2,793,284 - +0.0 2,793,284 - +0.2 2,793,284 - +0.2 2,793,286 + -0.9 2,793,286 + -0.8 2,793,287 - -1.4 2,793,496 + -0.1 2,793,496 + +0.5 2,793,497 - -0.3 2,793,550 + AMB_RS13120 0.15 -0.1 2,793,551 - AMB_RS13120 0.15 +0.1 2,793,739 + AMB_RS13120 0.34 -0.5 2,793,740 - AMB_RS13120 0.34 -0.0 2,793,740 - AMB_RS13120 0.34 -0.0 2,793,799 + AMB_RS13120 0.40 -1.8 2,793,800 - AMB_RS13120 0.40 +0.7 2,793,800 - AMB_RS13120 0.40 -0.0 2,793,800 - AMB_RS13120 0.40 +0.1
Or see this region's nucleotide sequence