Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12020 and AMB_RS12025 overlap by 8 nucleotides AMB_RS12025 and AMB_RS12030 are separated by 11 nucleotides AMB_RS12030 and AMB_RS12035 are separated by 4 nucleotides AMB_RS12035 and AMB_RS12040 are separated by 56 nucleotides AMB_RS12040 and AMB_RS12045 are separated by 38 nucleotides
AMB_RS12020: AMB_RS12020 - DNA gyrase subunit A, at 2,587,960 to 2,590,647
_RS12020
AMB_RS12025: AMB_RS12025 - pantetheine-phosphate adenylyltransferase, at 2,590,640 to 2,591,146
_RS12025
AMB_RS12030: AMB_RS12030 - peptidylprolyl isomerase, at 2,591,158 to 2,591,634
_RS12030
AMB_RS12035: AMB_RS12035 - peptidylprolyl isomerase, at 2,591,639 to 2,592,184
_RS12035
AMB_RS12040: AMB_RS12040 - tRNA-Lys, at 2,592,241 to 2,592,316
_RS12040
AMB_RS12045: AMB_RS12045 - hypothetical protein, at 2,592,355 to 2,592,771
_RS12045
Position (kb)
2591
2592
2593 Strain fitness (log2 ratio)
-2
-1
0
1 at 2591.179 kb on + strand at 2591.180 kb on - strand at 2591.180 kb on - strand at 2591.372 kb on + strand, within AMB_RS12030 at 2591.372 kb on + strand, within AMB_RS12030 at 2591.373 kb on - strand, within AMB_RS12030 at 2591.373 kb on - strand, within AMB_RS12030 at 2591.373 kb on - strand, within AMB_RS12030 at 2591.485 kb on + strand, within AMB_RS12030 at 2591.501 kb on - strand, within AMB_RS12030 at 2591.501 kb on - strand, within AMB_RS12030 at 2591.636 kb on - strand at 2591.636 kb on - strand at 2591.636 kb on - strand at 2591.825 kb on + strand, within AMB_RS12035 at 2591.825 kb on + strand, within AMB_RS12035 at 2591.825 kb on + strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.826 kb on - strand, within AMB_RS12035 at 2591.838 kb on + strand, within AMB_RS12035 at 2591.839 kb on - strand, within AMB_RS12035 at 2592.047 kb on + strand, within AMB_RS12035 at 2592.047 kb on + strand, within AMB_RS12035 at 2592.050 kb on - strand, within AMB_RS12035 at 2592.143 kb on - strand at 2592.357 kb on - strand at 2592.489 kb on + strand, within AMB_RS12045 at 2592.565 kb on - strand, within AMB_RS12045 at 2592.832 kb on - strand at 2592.873 kb on - strand at 2592.873 kb on - strand at 2592.931 kb on - strand at 2592.939 kb on + strand at 2592.973 kb on - strand at 2593.023 kb on - strand at 2593.155 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 2,591,179 + -0.1 2,591,180 - -0.1 2,591,180 - -1.6 2,591,372 + AMB_RS12030 0.45 -0.1 2,591,372 + AMB_RS12030 0.45 -0.3 2,591,373 - AMB_RS12030 0.45 -0.6 2,591,373 - AMB_RS12030 0.45 +0.9 2,591,373 - AMB_RS12030 0.45 +0.9 2,591,485 + AMB_RS12030 0.69 +0.7 2,591,501 - AMB_RS12030 0.72 -1.3 2,591,501 - AMB_RS12030 0.72 -0.2 2,591,636 - -1.0 2,591,636 - +0.8 2,591,636 - -0.1 2,591,825 + AMB_RS12035 0.34 -0.3 2,591,825 + AMB_RS12035 0.34 +0.2 2,591,825 + AMB_RS12035 0.34 -1.1 2,591,826 - AMB_RS12035 0.34 +0.2 2,591,826 - AMB_RS12035 0.34 +1.0 2,591,826 - AMB_RS12035 0.34 -2.0 2,591,826 - AMB_RS12035 0.34 +1.2 2,591,826 - AMB_RS12035 0.34 +0.1 2,591,826 - AMB_RS12035 0.34 +0.2 2,591,838 + AMB_RS12035 0.36 -1.1 2,591,839 - AMB_RS12035 0.37 +0.4 2,592,047 + AMB_RS12035 0.75 -0.3 2,592,047 + AMB_RS12035 0.75 +0.7 2,592,050 - AMB_RS12035 0.75 -0.6 2,592,143 - -0.5 2,592,357 - +0.2 2,592,489 + AMB_RS12045 0.32 +0.5 2,592,565 - AMB_RS12045 0.50 +0.5 2,592,832 - -2.1 2,592,873 - -0.5 2,592,873 - +0.2 2,592,931 - -0.3 2,592,939 + -2.3 2,592,973 - -1.6 2,593,023 - -0.3 2,593,155 - -0.8
Or see this region's nucleotide sequence