Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10765 and AMB_RS10770 are separated by 77 nucleotides AMB_RS10770 and AMB_RS10775 are separated by 90 nucleotides AMB_RS10775 and AMB_RS10780 are separated by 52 nucleotides
AMB_RS10765: AMB_RS10765 - septal ring lytic transglycosylase RlpA family protein, at 2,306,226 to 2,306,597
_RS10765
AMB_RS10770: AMB_RS10770 - hypothetical protein, at 2,306,675 to 2,307,139
_RS10770
AMB_RS10775: AMB_RS10775 - CoA transferase, at 2,307,230 to 2,308,438
_RS10775
AMB_RS10780: AMB_RS10780 - methyl-accepting chemotaxis protein, at 2,308,491 to 2,310,443
_RS10780
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2306.307 kb on + strand, within AMB_RS10765 at 2306.307 kb on + strand, within AMB_RS10765 at 2306.307 kb on + strand, within AMB_RS10765 at 2306.308 kb on - strand, within AMB_RS10765 at 2306.308 kb on - strand, within AMB_RS10765 at 2306.399 kb on + strand, within AMB_RS10765 at 2306.400 kb on - strand, within AMB_RS10765 at 2306.400 kb on - strand, within AMB_RS10765 at 2306.431 kb on + strand, within AMB_RS10765 at 2306.487 kb on + strand, within AMB_RS10765 at 2306.487 kb on + strand, within AMB_RS10765 at 2306.488 kb on - strand, within AMB_RS10765 at 2306.488 kb on - strand, within AMB_RS10765 at 2306.547 kb on + strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.595 kb on + strand at 2306.660 kb on - strand at 2306.677 kb on - strand at 2306.677 kb on - strand at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.822 kb on + strand, within AMB_RS10775 at 2307.822 kb on + strand, within AMB_RS10775 at 2307.822 kb on + strand, within AMB_RS10775 at 2307.823 kb on - strand, within AMB_RS10775 at 2307.823 kb on - strand, within AMB_RS10775 at 2307.915 kb on + strand, within AMB_RS10775 at 2307.915 kb on + strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.976 kb on - strand, within AMB_RS10775 at 2308.029 kb on + strand, within AMB_RS10775 at 2308.030 kb on - strand, within AMB_RS10775 at 2308.047 kb on + strand, within AMB_RS10775 at 2308.048 kb on - strand, within AMB_RS10775 at 2308.368 kb on + strand at 2308.368 kb on + strand at 2308.408 kb on + strand at 2308.409 kb on - strand at 2308.492 kb on + strand at 2308.493 kb on - strand at 2308.493 kb on - strand at 2308.493 kb on - strand at 2308.604 kb on + strand at 2308.604 kb on + strand at 2308.631 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 2,306,307 + AMB_RS10765 0.22 -1.0 2,306,307 + AMB_RS10765 0.22 +0.0 2,306,307 + AMB_RS10765 0.22 -3.8 2,306,308 - AMB_RS10765 0.22 +0.6 2,306,308 - AMB_RS10765 0.22 -0.0 2,306,399 + AMB_RS10765 0.47 -0.1 2,306,400 - AMB_RS10765 0.47 -0.3 2,306,400 - AMB_RS10765 0.47 +0.2 2,306,431 + AMB_RS10765 0.55 -0.6 2,306,487 + AMB_RS10765 0.70 +1.0 2,306,487 + AMB_RS10765 0.70 -0.8 2,306,488 - AMB_RS10765 0.70 -0.3 2,306,488 - AMB_RS10765 0.70 +0.1 2,306,547 + AMB_RS10765 0.86 +0.6 2,306,548 - AMB_RS10765 0.87 -0.2 2,306,548 - AMB_RS10765 0.87 +0.2 2,306,548 - AMB_RS10765 0.87 -1.3 2,306,548 - AMB_RS10765 0.87 +0.1 2,306,595 + -0.8 2,306,660 - +0.7 2,306,677 - -2.3 2,306,677 - -0.9 2,306,766 + AMB_RS10770 0.20 -0.4 2,306,766 + AMB_RS10770 0.20 -0.3 2,306,766 + AMB_RS10770 0.20 -0.6 2,306,766 + AMB_RS10770 0.20 +0.4 2,306,767 - AMB_RS10770 0.20 -1.8 2,306,767 - AMB_RS10770 0.20 -0.3 2,306,767 - AMB_RS10770 0.20 +0.0 2,306,767 - AMB_RS10770 0.20 -1.2 2,307,250 + +1.7 2,307,250 + +1.2 2,307,250 + -1.4 2,307,250 + -0.9 2,307,822 + AMB_RS10775 0.49 -1.1 2,307,822 + AMB_RS10775 0.49 -0.8 2,307,822 + AMB_RS10775 0.49 -1.4 2,307,823 - AMB_RS10775 0.49 +0.6 2,307,823 - AMB_RS10775 0.49 +0.1 2,307,915 + AMB_RS10775 0.57 -0.6 2,307,915 + AMB_RS10775 0.57 -2.1 2,307,916 - AMB_RS10775 0.57 -0.8 2,307,916 - AMB_RS10775 0.57 -1.3 2,307,916 - AMB_RS10775 0.57 +0.6 2,307,916 - AMB_RS10775 0.57 +0.8 2,307,975 + AMB_RS10775 0.62 -0.6 2,307,975 + AMB_RS10775 0.62 +0.6 2,307,975 + AMB_RS10775 0.62 +0.2 2,307,976 - AMB_RS10775 0.62 -2.3 2,308,029 + AMB_RS10775 0.66 -0.8 2,308,030 - AMB_RS10775 0.66 -0.5 2,308,047 + AMB_RS10775 0.68 +1.7 2,308,048 - AMB_RS10775 0.68 -1.3 2,308,368 + -0.2 2,308,368 + -0.8 2,308,408 + -0.2 2,308,409 - -0.3 2,308,492 + +0.8 2,308,493 - -0.6 2,308,493 - +0.6 2,308,493 - -0.2 2,308,604 + -0.2 2,308,604 + -0.4 2,308,631 + -0.6
Or see this region's nucleotide sequence