Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05675 and AMB_RS05680 are separated by 43 nucleotides AMB_RS05680 and AMB_RS05685 are separated by 24 nucleotides AMB_RS05685 and AMB_RS05690 are separated by 155 nucleotides AMB_RS05690 and AMB_RS23250 are separated by 146 nucleotides
AMB_RS05675: AMB_RS05675 - Serine phosphatase RsbU regulator sigma subunit, at 1,190,898 to 1,192,511
_RS05675
AMB_RS05680: AMB_RS05680 - anti-sigma factor antagonist, at 1,192,555 to 1,192,941
_RS05680
AMB_RS05685: AMB_RS05685 - D-alanyl-D-alanine carboxypeptidase, at 1,192,966 to 1,194,273
_RS05685
AMB_RS05690: AMB_RS05690 - hypothetical protein, at 1,194,429 to 1,195,103
_RS05690
AMB_RS23250: AMB_RS23250 - hypothetical protein, at 1,195,250 to 1,196,068
_RS23250
Position (kb)
1192
1193
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.978 kb on + strand, within AMB_RS05675 at 1191.979 kb on - strand, within AMB_RS05675 at 1191.979 kb on - strand, within AMB_RS05675 at 1191.979 kb on - strand, within AMB_RS05675 at 1192.107 kb on + strand, within AMB_RS05675 at 1192.107 kb on + strand, within AMB_RS05675 at 1192.108 kb on - strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.143 kb on + strand, within AMB_RS05675 at 1192.144 kb on - strand, within AMB_RS05675 at 1192.144 kb on - strand, within AMB_RS05675 at 1192.144 kb on - strand, within AMB_RS05675 at 1192.251 kb on + strand, within AMB_RS05675 at 1192.252 kb on - strand, within AMB_RS05675 at 1192.252 kb on - strand, within AMB_RS05675 at 1192.299 kb on + strand, within AMB_RS05675 at 1192.509 kb on + strand at 1192.509 kb on + strand at 1192.510 kb on - strand at 1192.539 kb on - strand at 1192.539 kb on - strand at 1192.604 kb on - strand, within AMB_RS05680 at 1192.614 kb on + strand, within AMB_RS05680 at 1192.615 kb on - strand, within AMB_RS05680 at 1192.615 kb on - strand, within AMB_RS05680 at 1192.825 kb on + strand, within AMB_RS05680 at 1194.349 kb on - strand at 1194.349 kb on - strand at 1194.378 kb on + strand at 1194.390 kb on + strand at 1194.424 kb on - strand at 1194.492 kb on + strand at 1194.492 kb on + strand at 1194.492 kb on + strand at 1194.493 kb on - strand at 1194.522 kb on + strand, within AMB_RS05690 at 1194.612 kb on + strand, within AMB_RS05690 at 1194.612 kb on + strand, within AMB_RS05690 at 1194.613 kb on - strand, within AMB_RS05690 at 1194.613 kb on - strand, within AMB_RS05690 at 1194.646 kb on - strand, within AMB_RS05690 at 1194.646 kb on - strand, within AMB_RS05690 at 1194.781 kb on - strand, within AMB_RS05690 at 1194.781 kb on - strand, within AMB_RS05690 at 1194.781 kb on - strand, within AMB_RS05690 at 1194.781 kb on - strand, within AMB_RS05690 at 1194.864 kb on + strand, within AMB_RS05690 at 1194.865 kb on - strand, within AMB_RS05690 at 1194.897 kb on + strand, within AMB_RS05690 at 1194.898 kb on - strand, within AMB_RS05690 at 1194.898 kb on - strand, within AMB_RS05690 at 1195.032 kb on + strand, within AMB_RS05690 at 1195.033 kb on - strand, within AMB_RS05690 at 1195.119 kb on + strand at 1195.126 kb on - strand at 1195.178 kb on - strand at 1195.178 kb on - strand at 1195.212 kb on + strand at 1195.213 kb on - strand at 1195.213 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 1,191,978 + AMB_RS05675 0.67 +0.2 1,191,978 + AMB_RS05675 0.67 -0.4 1,191,978 + AMB_RS05675 0.67 +0.5 1,191,978 + AMB_RS05675 0.67 -1.5 1,191,978 + AMB_RS05675 0.67 +0.5 1,191,978 + AMB_RS05675 0.67 -1.0 1,191,979 - AMB_RS05675 0.67 +0.2 1,191,979 - AMB_RS05675 0.67 -0.5 1,191,979 - AMB_RS05675 0.67 +0.4 1,192,107 + AMB_RS05675 0.75 +1.0 1,192,107 + AMB_RS05675 0.75 -0.7 1,192,108 - AMB_RS05675 0.75 -0.3 1,192,143 + AMB_RS05675 0.77 +0.3 1,192,143 + AMB_RS05675 0.77 +0.2 1,192,143 + AMB_RS05675 0.77 +0.1 1,192,143 + AMB_RS05675 0.77 -0.3 1,192,143 + AMB_RS05675 0.77 -0.2 1,192,143 + AMB_RS05675 0.77 +0.4 1,192,144 - AMB_RS05675 0.77 -0.8 1,192,144 - AMB_RS05675 0.77 -0.6 1,192,144 - AMB_RS05675 0.77 -0.6 1,192,251 + AMB_RS05675 0.84 +0.3 1,192,252 - AMB_RS05675 0.84 -0.8 1,192,252 - AMB_RS05675 0.84 -0.5 1,192,299 + AMB_RS05675 0.87 -0.5 1,192,509 + -0.2 1,192,509 + -0.3 1,192,510 - -0.4 1,192,539 - +0.1 1,192,539 - +0.9 1,192,604 - AMB_RS05680 0.13 -0.2 1,192,614 + AMB_RS05680 0.15 -1.7 1,192,615 - AMB_RS05680 0.16 -0.1 1,192,615 - AMB_RS05680 0.16 -0.3 1,192,825 + AMB_RS05680 0.70 -0.0 1,194,349 - -1.1 1,194,349 - -0.9 1,194,378 + -2.1 1,194,390 + +0.5 1,194,424 - -0.5 1,194,492 + -0.8 1,194,492 + -0.3 1,194,492 + +0.1 1,194,493 - +0.2 1,194,522 + AMB_RS05690 0.14 -0.8 1,194,612 + AMB_RS05690 0.27 -1.1 1,194,612 + AMB_RS05690 0.27 +0.7 1,194,613 - AMB_RS05690 0.27 +0.4 1,194,613 - AMB_RS05690 0.27 -0.6 1,194,646 - AMB_RS05690 0.32 +0.9 1,194,646 - AMB_RS05690 0.32 +0.7 1,194,781 - AMB_RS05690 0.52 -0.2 1,194,781 - AMB_RS05690 0.52 +0.6 1,194,781 - AMB_RS05690 0.52 +0.4 1,194,781 - AMB_RS05690 0.52 -0.1 1,194,864 + AMB_RS05690 0.64 +0.8 1,194,865 - AMB_RS05690 0.65 +0.1 1,194,897 + AMB_RS05690 0.69 -0.5 1,194,898 - AMB_RS05690 0.69 -0.6 1,194,898 - AMB_RS05690 0.69 +0.2 1,195,032 + AMB_RS05690 0.89 -0.2 1,195,033 - AMB_RS05690 0.89 -0.1 1,195,119 + +0.9 1,195,126 - +0.2 1,195,178 - -0.1 1,195,178 - +1.0 1,195,212 + +1.5 1,195,213 - +0.9 1,195,213 - +0.2
Or see this region's nucleotide sequence