Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03985 and AMB_RS03990 are separated by 1 nucleotides AMB_RS03990 and AMB_RS03995 overlap by 4 nucleotides AMB_RS03995 and AMB_RS04000 overlap by 36 nucleotides AMB_RS04000 and AMB_RS04005 are separated by 346 nucleotides
AMB_RS03985: AMB_RS03985 - bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase, at 835,689 to 837,155
_RS03985
AMB_RS03990: AMB_RS03990 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 837,157 to 837,618
_RS03990
AMB_RS03995: AMB_RS03995 - tRNA epoxyqueuosine(34) reductase QueG, at 837,615 to 838,697
_RS03995
AMB_RS04000: AMB_RS04000 - XRE family transcriptional regulator, at 838,662 to 839,054
_RS04000
AMB_RS04005: AMB_RS04005 - hypothetical protein, at 839,401 to 839,871
_RS04005
Position (kb) 837
838
839 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 837.268 kb on + strand, within AMB_RS03990 at 837.268 kb on + strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.269 kb on - strand, within AMB_RS03990 at 837.352 kb on + strand, within AMB_RS03990 at 837.760 kb on - strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.876 kb on + strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.877 kb on - strand, within AMB_RS03995 at 837.929 kb on + strand, within AMB_RS03995 at 837.939 kb on + strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.940 kb on - strand, within AMB_RS03995 at 837.957 kb on + strand, within AMB_RS03995 at 838.112 kb on + strand, within AMB_RS03995 at 838.290 kb on + strand, within AMB_RS03995 at 838.291 kb on - strand, within AMB_RS03995 at 838.356 kb on + strand, within AMB_RS03995 at 838.356 kb on + strand, within AMB_RS03995 at 838.356 kb on + strand, within AMB_RS03995 at 838.533 kb on + strand, within AMB_RS03995 at 838.810 kb on + strand, within AMB_RS04000 at 838.810 kb on + strand, within AMB_RS04000 at 838.810 kb on + strand, within AMB_RS04000 at 838.811 kb on - strand, within AMB_RS04000 at 838.811 kb on - strand, within AMB_RS04000 at 838.811 kb on - strand, within AMB_RS04000 at 838.811 kb on - strand, within AMB_RS04000 at 838.891 kb on + strand, within AMB_RS04000 at 838.891 kb on + strand, within AMB_RS04000 at 838.892 kb on - strand, within AMB_RS04000 at 838.892 kb on - strand, within AMB_RS04000 at 838.895 kb on + strand, within AMB_RS04000 at 838.895 kb on + strand, within AMB_RS04000 at 838.895 kb on + strand, within AMB_RS04000 at 838.896 kb on - strand, within AMB_RS04000 at 838.948 kb on - strand, within AMB_RS04000 at 839.122 kb on - strand at 839.122 kb on - strand at 839.137 kb on + strand at 839.138 kb on - strand at 839.176 kb on + strand at 839.198 kb on + strand at 839.239 kb on - strand at 839.327 kb on + strand at 839.327 kb on + strand at 839.328 kb on - strand at 839.328 kb on - strand at 839.331 kb on + strand at 839.332 kb on - strand at 839.374 kb on + strand at 839.388 kb on + strand at 839.388 kb on + strand at 839.389 kb on - strand at 839.403 kb on - strand at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.462 kb on + strand, within AMB_RS04005 at 839.463 kb on - strand, within AMB_RS04005 at 839.463 kb on - strand, within AMB_RS04005 at 839.519 kb on + strand, within AMB_RS04005 at 839.535 kb on - strand, within AMB_RS04005 at 839.535 kb on - strand, within AMB_RS04005
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 837,268 + AMB_RS03990 0.24 +0.2 837,268 + AMB_RS03990 0.24 +1.8 837,269 - AMB_RS03990 0.24 -0.6 837,269 - AMB_RS03990 0.24 -0.5 837,269 - AMB_RS03990 0.24 -0.7 837,352 + AMB_RS03990 0.42 -1.8 837,760 - AMB_RS03995 0.13 -0.7 837,876 + AMB_RS03995 0.24 +0.4 837,876 + AMB_RS03995 0.24 +0.2 837,876 + AMB_RS03995 0.24 +0.3 837,877 - AMB_RS03995 0.24 +0.1 837,877 - AMB_RS03995 0.24 -0.6 837,877 - AMB_RS03995 0.24 -0.8 837,877 - AMB_RS03995 0.24 -0.0 837,929 + AMB_RS03995 0.29 -0.4 837,939 + AMB_RS03995 0.30 -0.4 837,940 - AMB_RS03995 0.30 +0.0 837,940 - AMB_RS03995 0.30 +0.0 837,940 - AMB_RS03995 0.30 -0.5 837,957 + AMB_RS03995 0.32 -0.4 838,112 + AMB_RS03995 0.46 -1.3 838,290 + AMB_RS03995 0.62 +0.4 838,291 - AMB_RS03995 0.62 -0.4 838,356 + AMB_RS03995 0.68 -0.6 838,356 + AMB_RS03995 0.68 -1.1 838,356 + AMB_RS03995 0.68 -0.8 838,533 + AMB_RS03995 0.85 +0.2 838,810 + AMB_RS04000 0.38 -0.8 838,810 + AMB_RS04000 0.38 +0.5 838,810 + AMB_RS04000 0.38 -0.3 838,811 - AMB_RS04000 0.38 +0.8 838,811 - AMB_RS04000 0.38 -0.9 838,811 - AMB_RS04000 0.38 -1.3 838,811 - AMB_RS04000 0.38 -0.5 838,891 + AMB_RS04000 0.58 -0.3 838,891 + AMB_RS04000 0.58 -0.3 838,892 - AMB_RS04000 0.59 -0.8 838,892 - AMB_RS04000 0.59 -0.9 838,895 + AMB_RS04000 0.59 +0.1 838,895 + AMB_RS04000 0.59 +0.2 838,895 + AMB_RS04000 0.59 -0.4 838,896 - AMB_RS04000 0.60 +0.4 838,948 - AMB_RS04000 0.73 -0.2 839,122 - +0.6 839,122 - +0.0 839,137 + +0.2 839,138 - -0.6 839,176 + -0.1 839,198 + -0.8 839,239 - -0.5 839,327 + -0.0 839,327 + -0.4 839,328 - -0.3 839,328 - -0.3 839,331 + +0.2 839,332 - -0.8 839,374 + +0.2 839,388 + -1.5 839,388 + -0.6 839,389 - -0.9 839,403 - -1.3 839,462 + AMB_RS04005 0.13 -0.3 839,462 + AMB_RS04005 0.13 -0.9 839,462 + AMB_RS04005 0.13 +0.2 839,462 + AMB_RS04005 0.13 -1.1 839,463 - AMB_RS04005 0.13 +0.9 839,463 - AMB_RS04005 0.13 +0.2 839,519 + AMB_RS04005 0.25 -3.3 839,535 - AMB_RS04005 0.28 -0.1 839,535 - AMB_RS04005 0.28 +1.1
Or see this region's nucleotide sequence