Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03605 and AMB_RS03610 are separated by 17 nucleotides AMB_RS03610 and AMB_RS03615 are separated by 12 nucleotides AMB_RS03615 and AMB_RS03620 overlap by 4 nucleotides AMB_RS03620 and AMB_RS03625 are separated by 54 nucleotides
AMB_RS03605: AMB_RS03605 - carbamoyl-phosphate synthase large subunit, at 757,477 to 760,734
_RS03605
AMB_RS03610: AMB_RS03610 - transcription elongation factor GreA, at 760,752 to 761,225
_RS03610
AMB_RS03615: AMB_RS03615 - hypothetical protein, at 761,238 to 761,660
_RS03615
AMB_RS03620: AMB_RS03620 - iron-dependent peroxidase, at 761,657 to 762,559
_RS03620
AMB_RS03625: AMB_RS03625 - hypothetical protein, at 762,614 to 763,990
_RS03625
Position (kb) 761
762
763 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 760.714 kb on + strand at 760.719 kb on + strand at 760.720 kb on - strand at 760.720 kb on - strand at 760.740 kb on + strand at 760.741 kb on - strand at 760.805 kb on + strand, within AMB_RS03610 at 760.805 kb on + strand, within AMB_RS03610 at 760.805 kb on + strand, within AMB_RS03610 at 760.805 kb on + strand, within AMB_RS03610 at 760.805 kb on + strand, within AMB_RS03610 at 760.806 kb on - strand, within AMB_RS03610 at 760.806 kb on - strand, within AMB_RS03610 at 760.806 kb on - strand, within AMB_RS03610 at 760.806 kb on - strand, within AMB_RS03610 at 760.806 kb on - strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.818 kb on + strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 760.819 kb on - strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.061 kb on + strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.062 kb on - strand, within AMB_RS03610 at 761.165 kb on - strand, within AMB_RS03610 at 761.165 kb on - strand, within AMB_RS03610 at 761.197 kb on - strand at 761.286 kb on + strand, within AMB_RS03615 at 761.286 kb on + strand, within AMB_RS03615 at 761.355 kb on + strand, within AMB_RS03615 at 761.356 kb on - strand, within AMB_RS03615 at 761.356 kb on - strand, within AMB_RS03615 at 761.395 kb on + strand, within AMB_RS03615 at 761.635 kb on + strand at 761.636 kb on - strand at 761.636 kb on - strand at 762.188 kb on + strand, within AMB_RS03620 at 762.188 kb on + strand, within AMB_RS03620 at 762.189 kb on - strand, within AMB_RS03620 at 762.189 kb on - strand, within AMB_RS03620 at 762.189 kb on - strand, within AMB_RS03620 at 762.189 kb on - strand, within AMB_RS03620 at 762.365 kb on + strand, within AMB_RS03620 at 762.392 kb on + strand, within AMB_RS03620 at 762.392 kb on + strand, within AMB_RS03620 at 762.392 kb on + strand, within AMB_RS03620 at 762.392 kb on + strand, within AMB_RS03620 at 762.393 kb on - strand, within AMB_RS03620 at 762.393 kb on - strand, within AMB_RS03620 at 762.393 kb on - strand, within AMB_RS03620 at 762.404 kb on + strand, within AMB_RS03620 at 762.405 kb on - strand, within AMB_RS03620 at 762.719 kb on + strand at 762.720 kb on - strand at 762.720 kb on - strand at 762.720 kb on - strand at 762.720 kb on - strand at 763.409 kb on + strand, within AMB_RS03625 at 763.409 kb on + strand, within AMB_RS03625 at 763.409 kb on + strand, within AMB_RS03625 at 763.409 kb on + strand, within AMB_RS03625 at 763.410 kb on - strand, within AMB_RS03625 at 763.410 kb on - strand, within AMB_RS03625 at 763.410 kb on - strand, within AMB_RS03625
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 760,714 + +1.8 760,719 + +2.3 760,720 - -1.3 760,720 - -0.0 760,740 + -0.7 760,741 - +1.8 760,805 + AMB_RS03610 0.11 -1.3 760,805 + AMB_RS03610 0.11 -1.0 760,805 + AMB_RS03610 0.11 -0.7 760,805 + AMB_RS03610 0.11 -0.5 760,805 + AMB_RS03610 0.11 -1.7 760,806 - AMB_RS03610 0.11 -0.2 760,806 - AMB_RS03610 0.11 +1.3 760,806 - AMB_RS03610 0.11 +0.3 760,806 - AMB_RS03610 0.11 -0.5 760,806 - AMB_RS03610 0.11 -1.3 760,818 + AMB_RS03610 0.14 +0.8 760,818 + AMB_RS03610 0.14 -2.0 760,818 + AMB_RS03610 0.14 -0.4 760,818 + AMB_RS03610 0.14 +1.0 760,818 + AMB_RS03610 0.14 +1.0 760,818 + AMB_RS03610 0.14 +1.8 760,818 + AMB_RS03610 0.14 -0.2 760,818 + AMB_RS03610 0.14 -1.9 760,818 + AMB_RS03610 0.14 -0.4 760,818 + AMB_RS03610 0.14 -0.6 760,819 - AMB_RS03610 0.14 +1.7 760,819 - AMB_RS03610 0.14 -0.5 760,819 - AMB_RS03610 0.14 +0.6 760,819 - AMB_RS03610 0.14 -1.3 760,819 - AMB_RS03610 0.14 -1.3 760,819 - AMB_RS03610 0.14 -0.5 760,819 - AMB_RS03610 0.14 -0.1 760,819 - AMB_RS03610 0.14 +0.8 761,061 + AMB_RS03610 0.65 +1.6 761,061 + AMB_RS03610 0.65 +1.1 761,061 + AMB_RS03610 0.65 +1.3 761,061 + AMB_RS03610 0.65 -0.2 761,061 + AMB_RS03610 0.65 +0.3 761,061 + AMB_RS03610 0.65 +0.1 761,062 - AMB_RS03610 0.65 +0.6 761,062 - AMB_RS03610 0.65 +1.3 761,062 - AMB_RS03610 0.65 +0.1 761,062 - AMB_RS03610 0.65 -0.3 761,062 - AMB_RS03610 0.65 -0.1 761,062 - AMB_RS03610 0.65 -1.1 761,165 - AMB_RS03610 0.87 -3.2 761,165 - AMB_RS03610 0.87 -0.1 761,197 - -0.7 761,286 + AMB_RS03615 0.11 +0.3 761,286 + AMB_RS03615 0.11 -0.5 761,355 + AMB_RS03615 0.28 -1.8 761,356 - AMB_RS03615 0.28 -1.1 761,356 - AMB_RS03615 0.28 -1.2 761,395 + AMB_RS03615 0.37 -0.3 761,635 + -0.2 761,636 - +0.1 761,636 - +0.4 762,188 + AMB_RS03620 0.59 -0.6 762,188 + AMB_RS03620 0.59 +0.8 762,189 - AMB_RS03620 0.59 +1.0 762,189 - AMB_RS03620 0.59 +0.1 762,189 - AMB_RS03620 0.59 -0.3 762,189 - AMB_RS03620 0.59 -0.1 762,365 + AMB_RS03620 0.78 -0.0 762,392 + AMB_RS03620 0.81 -0.3 762,392 + AMB_RS03620 0.81 -0.6 762,392 + AMB_RS03620 0.81 -2.7 762,392 + AMB_RS03620 0.81 -0.2 762,393 - AMB_RS03620 0.82 +0.1 762,393 - AMB_RS03620 0.82 -2.3 762,393 - AMB_RS03620 0.82 -1.7 762,404 + AMB_RS03620 0.83 +0.6 762,405 - AMB_RS03620 0.83 +1.7 762,719 + +0.5 762,720 - -0.7 762,720 - -0.6 762,720 - -2.3 762,720 - -0.1 763,409 + AMB_RS03625 0.58 -1.2 763,409 + AMB_RS03625 0.58 -0.9 763,409 + AMB_RS03625 0.58 -0.5 763,409 + AMB_RS03625 0.58 -0.4 763,410 - AMB_RS03625 0.58 -0.3 763,410 - AMB_RS03625 0.58 +0.5 763,410 - AMB_RS03625 0.58 +2.3
Or see this region's nucleotide sequence