Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03225 and AMB_RS03230 are separated by 287 nucleotides AMB_RS03230 and AMB_RS03235 are separated by 7 nucleotides AMB_RS03235 and AMB_RS03240 are separated by 91 nucleotides
AMB_RS03225: AMB_RS03225 - DedA family protein, at 674,758 to 675,336
_RS03225
AMB_RS03230: AMB_RS03230 - hypothetical protein, at 675,624 to 676,058
_RS03230
AMB_RS03235: AMB_RS03235 - flagellum-specific ATP synthase FliI, at 676,066 to 677,418
_RS03235
AMB_RS03240: AMB_RS03240 - DNA-binding response regulator, at 677,510 to 678,226
_RS03240
Position (kb)
676
677
678 Strain fitness (log2 ratio)
-2
-1
0
1 at 675.335 kb on - strand at 675.335 kb on - strand at 675.346 kb on + strand at 675.350 kb on + strand at 675.350 kb on + strand at 675.351 kb on - strand at 675.351 kb on - strand at 675.360 kb on + strand at 675.360 kb on + strand at 675.411 kb on + strand at 675.417 kb on - strand at 675.543 kb on - strand at 675.615 kb on + strand at 675.615 kb on + strand at 675.615 kb on + strand at 675.616 kb on - strand at 675.616 kb on - strand at 675.746 kb on - strand, within AMB_RS03230 at 675.871 kb on + strand, within AMB_RS03230 at 675.871 kb on + strand, within AMB_RS03230 at 675.872 kb on - strand, within AMB_RS03230 at 675.872 kb on - strand, within AMB_RS03230 at 675.872 kb on - strand, within AMB_RS03230 at 675.872 kb on - strand, within AMB_RS03230 at 676.067 kb on + strand at 676.067 kb on + strand at 676.068 kb on - strand at 676.127 kb on + strand at 676.127 kb on + strand at 676.128 kb on - strand at 676.128 kb on - strand at 676.128 kb on - strand at 676.128 kb on - strand at 676.191 kb on - strand at 676.193 kb on + strand at 676.194 kb on - strand at 676.194 kb on - strand at 676.271 kb on + strand, within AMB_RS03235 at 676.272 kb on - strand, within AMB_RS03235 at 676.546 kb on + strand, within AMB_RS03235 at 676.546 kb on + strand, within AMB_RS03235 at 676.547 kb on - strand, within AMB_RS03235 at 676.547 kb on - strand, within AMB_RS03235 at 676.547 kb on - strand, within AMB_RS03235 at 676.547 kb on - strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.569 kb on + strand, within AMB_RS03235 at 676.570 kb on - strand, within AMB_RS03235 at 676.570 kb on - strand, within AMB_RS03235 at 676.707 kb on + strand, within AMB_RS03235 at 676.707 kb on + strand, within AMB_RS03235 at 676.707 kb on + strand, within AMB_RS03235 at 676.707 kb on + strand, within AMB_RS03235 at 676.708 kb on - strand, within AMB_RS03235 at 676.708 kb on - strand, within AMB_RS03235 at 676.708 kb on - strand, within AMB_RS03235 at 676.708 kb on - strand, within AMB_RS03235 at 676.708 kb on - strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.709 kb on + strand, within AMB_RS03235 at 676.710 kb on - strand, within AMB_RS03235 at 676.710 kb on - strand, within AMB_RS03235 at 676.710 kb on - strand, within AMB_RS03235 at 676.710 kb on - strand, within AMB_RS03235 at 676.899 kb on + strand, within AMB_RS03235 at 676.899 kb on + strand, within AMB_RS03235 at 676.899 kb on + strand, within AMB_RS03235 at 676.900 kb on - strand, within AMB_RS03235 at 676.900 kb on - strand, within AMB_RS03235 at 677.198 kb on + strand, within AMB_RS03235 at 677.198 kb on + strand, within AMB_RS03235 at 677.198 kb on + strand, within AMB_RS03235 at 677.199 kb on - strand, within AMB_RS03235 at 677.357 kb on + strand at 677.457 kb on + strand at 677.457 kb on + strand at 677.457 kb on + strand at 677.458 kb on - strand at 677.465 kb on + strand at 677.465 kb on + strand at 677.471 kb on + strand at 677.472 kb on - strand at 677.481 kb on + strand at 677.481 kb on + strand at 677.481 kb on + strand at 677.482 kb on - strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.506 kb on + strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.507 kb on - strand at 677.636 kb on + strand, within AMB_RS03240 at 677.636 kb on + strand, within AMB_RS03240 at 677.637 kb on - strand, within AMB_RS03240 at 677.642 kb on + strand, within AMB_RS03240 at 677.687 kb on + strand, within AMB_RS03240 at 677.801 kb on + strand, within AMB_RS03240 at 677.802 kb on - strand, within AMB_RS03240 at 677.802 kb on - strand, within AMB_RS03240 at 678.033 kb on + strand, within AMB_RS03240 at 678.034 kb on - strand, within AMB_RS03240 at 678.034 kb on - strand, within AMB_RS03240 at 678.044 kb on + strand, within AMB_RS03240 at 678.044 kb on + strand, within AMB_RS03240 at 678.045 kb on - strand, within AMB_RS03240 at 678.045 kb on - strand, within AMB_RS03240 at 678.137 kb on + strand, within AMB_RS03240 at 678.137 kb on + strand, within AMB_RS03240 at 678.164 kb on + strand at 678.164 kb on + strand at 678.164 kb on + strand at 678.165 kb on - strand at 678.165 kb on - strand at 678.346 kb on - strand at 678.346 kb on - strand at 678.349 kb on + strand at 678.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 675,335 - -0.6 675,335 - -0.0 675,346 + +1.1 675,350 + +0.7 675,350 + +0.7 675,351 - -0.3 675,351 - -0.2 675,360 + -0.3 675,360 + -0.5 675,411 + -0.1 675,417 - -0.7 675,543 - -0.4 675,615 + +0.2 675,615 + -1.7 675,615 + -1.4 675,616 - -0.9 675,616 - -0.7 675,746 - AMB_RS03230 0.28 -2.3 675,871 + AMB_RS03230 0.57 -0.6 675,871 + AMB_RS03230 0.57 +0.4 675,872 - AMB_RS03230 0.57 -0.7 675,872 - AMB_RS03230 0.57 +0.2 675,872 - AMB_RS03230 0.57 +0.7 675,872 - AMB_RS03230 0.57 -0.1 676,067 + -0.2 676,067 + -0.4 676,068 - -0.5 676,127 + -0.3 676,127 + -0.6 676,128 - -0.5 676,128 - +0.7 676,128 - -1.0 676,128 - -0.3 676,191 - -0.1 676,193 + +1.0 676,194 - +0.5 676,194 - -0.4 676,271 + AMB_RS03235 0.15 -0.4 676,272 - AMB_RS03235 0.15 +0.4 676,546 + AMB_RS03235 0.35 +0.6 676,546 + AMB_RS03235 0.35 -0.7 676,547 - AMB_RS03235 0.36 -1.0 676,547 - AMB_RS03235 0.36 +0.3 676,547 - AMB_RS03235 0.36 -0.1 676,547 - AMB_RS03235 0.36 -0.6 676,569 + AMB_RS03235 0.37 -0.2 676,569 + AMB_RS03235 0.37 +0.7 676,569 + AMB_RS03235 0.37 -0.4 676,569 + AMB_RS03235 0.37 +0.2 676,569 + AMB_RS03235 0.37 +0.3 676,569 + AMB_RS03235 0.37 -0.2 676,570 - AMB_RS03235 0.37 -0.5 676,570 - AMB_RS03235 0.37 -0.7 676,707 + AMB_RS03235 0.47 +0.2 676,707 + AMB_RS03235 0.47 +0.4 676,707 + AMB_RS03235 0.47 -0.1 676,707 + AMB_RS03235 0.47 +0.6 676,708 - AMB_RS03235 0.47 -0.7 676,708 - AMB_RS03235 0.47 -0.5 676,708 - AMB_RS03235 0.47 +0.1 676,708 - AMB_RS03235 0.47 -0.9 676,708 - AMB_RS03235 0.47 +0.2 676,709 + AMB_RS03235 0.48 +0.3 676,709 + AMB_RS03235 0.48 -0.7 676,709 + AMB_RS03235 0.48 +1.2 676,709 + AMB_RS03235 0.48 -0.3 676,709 + AMB_RS03235 0.48 -0.4 676,709 + AMB_RS03235 0.48 -1.3 676,709 + AMB_RS03235 0.48 +0.4 676,709 + AMB_RS03235 0.48 -0.6 676,709 + AMB_RS03235 0.48 -0.4 676,709 + AMB_RS03235 0.48 -0.3 676,709 + AMB_RS03235 0.48 -0.7 676,709 + AMB_RS03235 0.48 -0.1 676,710 - AMB_RS03235 0.48 +1.1 676,710 - AMB_RS03235 0.48 -0.4 676,710 - AMB_RS03235 0.48 +0.0 676,710 - AMB_RS03235 0.48 -0.1 676,899 + AMB_RS03235 0.62 -1.3 676,899 + AMB_RS03235 0.62 -0.9 676,899 + AMB_RS03235 0.62 -2.1 676,900 - AMB_RS03235 0.62 +0.3 676,900 - AMB_RS03235 0.62 -0.1 677,198 + AMB_RS03235 0.84 -0.2 677,198 + AMB_RS03235 0.84 -1.9 677,198 + AMB_RS03235 0.84 +0.3 677,199 - AMB_RS03235 0.84 +0.3 677,357 + +1.0 677,457 + +0.3 677,457 + +1.1 677,457 + -0.5 677,458 - -0.4 677,465 + +1.7 677,465 + -0.3 677,471 + -0.2 677,472 - -0.1 677,481 + -0.7 677,481 + -1.7 677,481 + -0.6 677,482 - -0.4 677,506 + -0.4 677,506 + -1.8 677,506 + +0.3 677,506 + -1.2 677,507 - +0.5 677,507 - -0.2 677,507 - -0.7 677,507 - -0.1 677,636 + AMB_RS03240 0.18 -0.0 677,636 + AMB_RS03240 0.18 -1.0 677,637 - AMB_RS03240 0.18 -0.8 677,642 + AMB_RS03240 0.18 -0.4 677,687 + AMB_RS03240 0.25 -0.7 677,801 + AMB_RS03240 0.41 -1.0 677,802 - AMB_RS03240 0.41 +0.1 677,802 - AMB_RS03240 0.41 -0.3 678,033 + AMB_RS03240 0.73 +0.3 678,034 - AMB_RS03240 0.73 -0.2 678,034 - AMB_RS03240 0.73 -0.5 678,044 + AMB_RS03240 0.74 -1.2 678,044 + AMB_RS03240 0.74 +0.7 678,045 - AMB_RS03240 0.75 +0.3 678,045 - AMB_RS03240 0.75 -0.6 678,137 + AMB_RS03240 0.87 -0.4 678,137 + AMB_RS03240 0.87 -2.7 678,164 + -0.6 678,164 + -0.6 678,164 + -0.2 678,165 - -0.8 678,165 - -0.5 678,346 - -0.8 678,346 - -0.7 678,349 + -0.4 678,352 - +0.1
Or see this region's nucleotide sequence