Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02700 and AMB_RS02705 are separated by 95 nucleotides AMB_RS02705 and AMB_RS02710 are separated by 194 nucleotides AMB_RS02710 and AMB_RS02715 are separated by 104 nucleotides AMB_RS02715 and AMB_RS02720 overlap by 1 nucleotides
AMB_RS02700: AMB_RS02700 - carboxypeptidase, at 565,137 to 567,248
_RS02700
AMB_RS02705: AMB_RS02705 - ribbon-helix-helix protein, CopG family, at 567,344 to 567,547
_RS02705
AMB_RS02710: AMB_RS02710 - 2-nitropropane dioxygenase, at 567,742 to 569,139
_RS02710
AMB_RS02715: AMB_RS02715 - glycerophosphodiester phosphodiesterase, at 569,244 to 569,948
_RS02715
AMB_RS02720: AMB_RS02720 - glycosyltransferase family 9 protein, at 569,948 to 571,147
_RS02720
Position (kb) 567
568
569
570 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 566.782 kb on + strand, within AMB_RS02700 at 566.969 kb on - strand, within AMB_RS02700 at 566.972 kb on - strand, within AMB_RS02700 at 567.259 kb on - strand at 567.259 kb on - strand at 567.308 kb on + strand at 567.308 kb on + strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.309 kb on - strand at 567.329 kb on - strand at 567.455 kb on + strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.456 kb on - strand, within AMB_RS02705 at 567.627 kb on + strand at 567.723 kb on - strand at 567.743 kb on + strand at 567.744 kb on - strand at 569.165 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.202 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.204 kb on - strand at 569.206 kb on - strand at 569.206 kb on - strand at 569.206 kb on - strand at 569.206 kb on - strand at 569.206 kb on - strand at 569.327 kb on + strand, within AMB_RS02715 at 569.327 kb on + strand, within AMB_RS02715 at 569.327 kb on + strand, within AMB_RS02715 at 569.328 kb on - strand, within AMB_RS02715 at 569.328 kb on - strand, within AMB_RS02715 at 569.330 kb on - strand, within AMB_RS02715 at 569.426 kb on + strand, within AMB_RS02715 at 569.547 kb on + strand, within AMB_RS02715 at 569.547 kb on + strand, within AMB_RS02715
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 566,782 + AMB_RS02700 0.78 +0.5 566,969 - AMB_RS02700 0.87 +1.5 566,972 - AMB_RS02700 0.87 -0.2 567,259 - +0.6 567,259 - -0.3 567,308 + -0.5 567,308 + -2.3 567,309 - -0.2 567,309 - -0.3 567,309 - -0.6 567,309 - -0.4 567,309 - -0.0 567,329 - +0.1 567,455 + AMB_RS02705 0.54 -0.1 567,456 - AMB_RS02705 0.55 +0.6 567,456 - AMB_RS02705 0.55 -0.1 567,456 - AMB_RS02705 0.55 +0.4 567,456 - AMB_RS02705 0.55 -0.4 567,627 + -0.5 567,723 - -0.3 567,743 + +0.3 567,744 - -0.4 569,165 - +1.3 569,202 - +0.3 569,202 - +0.3 569,202 - -0.4 569,202 - -2.1 569,202 - -0.2 569,202 - +0.6 569,202 - +0.3 569,202 - +0.0 569,202 - -0.1 569,202 - +0.1 569,202 - -0.1 569,202 - -0.6 569,202 - -0.3 569,202 - +0.0 569,202 - +0.2 569,202 - +0.2 569,202 - -0.5 569,202 - +1.5 569,202 - +0.3 569,202 - +0.7 569,202 - -0.4 569,202 - +0.0 569,204 - +0.2 569,204 - +0.4 569,204 - +0.3 569,204 - +0.3 569,204 - +0.2 569,204 - -0.6 569,204 - +0.4 569,204 - -1.5 569,204 - -0.4 569,204 - -0.2 569,204 - +0.0 569,204 - -0.0 569,204 - +0.2 569,204 - +0.3 569,204 - +0.2 569,204 - -0.7 569,204 - -1.7 569,206 - -0.3 569,206 - -0.3 569,206 - -0.9 569,206 - -3.4 569,206 - +1.0 569,327 + AMB_RS02715 0.12 +0.0 569,327 + AMB_RS02715 0.12 -0.5 569,327 + AMB_RS02715 0.12 -0.4 569,328 - AMB_RS02715 0.12 -0.3 569,328 - AMB_RS02715 0.12 +0.1 569,330 - AMB_RS02715 0.12 +0.3 569,426 + AMB_RS02715 0.26 -0.7 569,547 + AMB_RS02715 0.43 -0.4 569,547 + AMB_RS02715 0.43 -0.6
Or see this region's nucleotide sequence