Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01505 and AMB_RS01510 overlap by 4 nucleotides AMB_RS01510 and AMB_RS01515 overlap by 10 nucleotides AMB_RS01515 and AMB_RS01520 are separated by 218 nucleotides
AMB_RS01505: AMB_RS01505 - bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase, at 332,655 to 333,830
_RS01505
AMB_RS01510: AMB_RS01510 - precorrin-2 C(20)-methyltransferase, at 333,827 to 334,525
_RS01510
AMB_RS01515: AMB_RS01515 - precorrin-3B C(17)-methyltransferase, at 334,516 to 336,309
_RS01515
AMB_RS01520: AMB_RS01520 - Zn-dependent protease, at 336,528 to 337,985
_RS01520
Position (kb) 334
335
336
337 Strain fitness (log2 ratio) -1
0
1 at 336.307 kb on + strand at 336.307 kb on + strand at 336.308 kb on - strand at 336.308 kb on - strand at 336.308 kb on - strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.310 kb on + strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.311 kb on - strand at 336.457 kb on - strand at 336.489 kb on + strand at 336.490 kb on - strand at 336.490 kb on - strand at 336.687 kb on + strand, within AMB_RS01520 at 336.723 kb on + strand, within AMB_RS01520 at 336.723 kb on + strand, within AMB_RS01520 at 336.724 kb on - strand, within AMB_RS01520 at 336.837 kb on + strand, within AMB_RS01520 at 336.837 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.843 kb on + strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.844 kb on - strand, within AMB_RS01520 at 336.948 kb on + strand, within AMB_RS01520 at 336.972 kb on - strand, within AMB_RS01520 at 337.059 kb on + strand, within AMB_RS01520 at 337.060 kb on - strand, within AMB_RS01520 at 337.060 kb on - strand, within AMB_RS01520 at 337.072 kb on - strand, within AMB_RS01520 at 337.116 kb on + strand, within AMB_RS01520 at 337.117 kb on - strand, within AMB_RS01520
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 336,307 + +0.9 336,307 + -0.4 336,308 - -0.2 336,308 - -0.7 336,308 - +1.0 336,310 + +0.8 336,310 + -0.4 336,310 + +0.5 336,310 + -1.0 336,311 - +1.7 336,311 - -1.1 336,311 - +0.7 336,311 - -0.1 336,457 - -0.2 336,489 + +0.3 336,490 - -0.9 336,490 - +0.5 336,687 + AMB_RS01520 0.11 -0.1 336,723 + AMB_RS01520 0.13 -0.3 336,723 + AMB_RS01520 0.13 +0.8 336,724 - AMB_RS01520 0.13 -0.0 336,837 + AMB_RS01520 0.21 -0.3 336,837 + AMB_RS01520 0.21 +0.9 336,843 + AMB_RS01520 0.22 -0.2 336,843 + AMB_RS01520 0.22 +0.0 336,843 + AMB_RS01520 0.22 -1.6 336,843 + AMB_RS01520 0.22 -0.2 336,844 - AMB_RS01520 0.22 -0.6 336,844 - AMB_RS01520 0.22 -0.1 336,844 - AMB_RS01520 0.22 +1.2 336,844 - AMB_RS01520 0.22 +0.4 336,844 - AMB_RS01520 0.22 +0.2 336,844 - AMB_RS01520 0.22 -0.4 336,948 + AMB_RS01520 0.29 -0.3 336,972 - AMB_RS01520 0.30 +0.3 337,059 + AMB_RS01520 0.36 -0.1 337,060 - AMB_RS01520 0.36 +0.5 337,060 - AMB_RS01520 0.36 +0.8 337,072 - AMB_RS01520 0.37 +0.5 337,116 + AMB_RS01520 0.40 +0.8 337,117 - AMB_RS01520 0.40 -0.4
Or see this region's nucleotide sequence