Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01280 and AMB_RS01285 overlap by 11 nucleotides AMB_RS01285 and AMB_RS01290 overlap by 4 nucleotides AMB_RS01290 and AMB_RS01295 are separated by 5 nucleotides AMB_RS01295 and AMB_RS01300 overlap by 1 nucleotides
AMB_RS01280: AMB_RS01280 - 4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase, at 283,684 to 284,379
_RS01280
AMB_RS01285: AMB_RS01285 - dehydrogenase, at 284,369 to 285,307
_RS01285
AMB_RS01290: AMB_RS01290 - 2-pyrone-4,6-dicarboxylate hydrolase, at 285,304 to 286,221
_RS01290
AMB_RS01295: AMB_RS01295 - gfo/Idh/MocA family oxidoreductase, at 286,227 to 287,189
_RS01295
AMB_RS01300: AMB_RS01300 - aldo/keto reductase, at 287,189 to 288,178
_RS01300
Position (kb) 285
286
287 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on + strand at 284.329 kb on - strand at 284.330 kb on - strand at 284.330 kb on - strand at 284.330 kb on - strand at 284.330 kb on - strand at 284.330 kb on - strand at 284.330 kb on - strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.368 kb on + strand at 284.369 kb on - strand at 284.369 kb on - strand at 284.369 kb on - strand at 284.377 kb on + strand at 284.483 kb on + strand, within AMB_RS01285 at 284.483 kb on + strand, within AMB_RS01285 at 284.483 kb on + strand, within AMB_RS01285 at 284.483 kb on + strand, within AMB_RS01285 at 284.783 kb on + strand, within AMB_RS01285 at 284.783 kb on + strand, within AMB_RS01285 at 284.784 kb on - strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.791 kb on + strand, within AMB_RS01285 at 284.792 kb on - strand, within AMB_RS01285 at 284.792 kb on - strand, within AMB_RS01285 at 284.792 kb on - strand, within AMB_RS01285 at 284.792 kb on - strand, within AMB_RS01285 at 284.978 kb on + strand, within AMB_RS01285 at 284.979 kb on - strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.053 kb on + strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.054 kb on - strand, within AMB_RS01285 at 285.191 kb on + strand, within AMB_RS01285 at 285.340 kb on + strand at 285.340 kb on + strand at 285.340 kb on + strand at 285.341 kb on - strand at 285.341 kb on - strand at 285.341 kb on - strand at 285.341 kb on - strand at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.448 kb on + strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.449 kb on - strand, within AMB_RS01290 at 285.687 kb on + strand, within AMB_RS01290 at 285.687 kb on + strand, within AMB_RS01290 at 285.687 kb on + strand, within AMB_RS01290 at 285.688 kb on - strand, within AMB_RS01290 at 285.688 kb on - strand, within AMB_RS01290 at 285.772 kb on + strand, within AMB_RS01290 at 285.773 kb on - strand, within AMB_RS01290 at 285.846 kb on + strand, within AMB_RS01290 at 285.846 kb on + strand, within AMB_RS01290 at 286.140 kb on + strand at 286.198 kb on + strand at 286.198 kb on + strand at 286.199 kb on - strand at 286.199 kb on - strand at 286.199 kb on - strand at 286.372 kb on - strand, within AMB_RS01295 at 286.561 kb on + strand, within AMB_RS01295 at 286.562 kb on - strand, within AMB_RS01295 at 286.686 kb on + strand, within AMB_RS01295 at 286.686 kb on + strand, within AMB_RS01295 at 286.687 kb on - strand, within AMB_RS01295 at 286.956 kb on + strand, within AMB_RS01295 at 286.957 kb on - strand, within AMB_RS01295 at 286.957 kb on - strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.977 kb on + strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.978 kb on - strand, within AMB_RS01295 at 286.990 kb on - strand, within AMB_RS01295 at 287.143 kb on - strand at 287.215 kb on + strand at 287.215 kb on + strand at 287.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 284,329 + +0.4 284,329 + +0.1 284,329 + -2.7 284,329 + +0.1 284,329 + -0.1 284,329 + -1.3 284,329 + -0.9 284,329 + -0.8 284,329 + +0.0 284,329 + +0.2 284,329 + +0.3 284,329 + +0.3 284,329 + -0.3 284,329 + -0.3 284,329 + -0.9 284,329 + -0.6 284,329 + -0.8 284,329 + -0.7 284,329 - +0.1 284,330 - +0.2 284,330 - +0.1 284,330 - +0.4 284,330 - -0.8 284,330 - +1.4 284,330 - +0.3 284,368 + -0.8 284,368 + +0.6 284,368 + -0.4 284,368 + +0.2 284,368 + -0.9 284,368 + -1.3 284,368 + -0.2 284,368 + -1.1 284,368 + +0.6 284,368 + +0.1 284,369 - -0.2 284,369 - -1.1 284,369 - +0.2 284,377 + -0.1 284,483 + AMB_RS01285 0.12 +0.0 284,483 + AMB_RS01285 0.12 -0.4 284,483 + AMB_RS01285 0.12 -0.6 284,483 + AMB_RS01285 0.12 -0.3 284,783 + AMB_RS01285 0.44 -0.7 284,783 + AMB_RS01285 0.44 -0.6 284,784 - AMB_RS01285 0.44 -0.6 284,791 + AMB_RS01285 0.45 +0.3 284,791 + AMB_RS01285 0.45 -0.5 284,791 + AMB_RS01285 0.45 -0.2 284,791 + AMB_RS01285 0.45 -0.6 284,791 + AMB_RS01285 0.45 -1.3 284,791 + AMB_RS01285 0.45 -0.6 284,792 - AMB_RS01285 0.45 +0.5 284,792 - AMB_RS01285 0.45 -0.2 284,792 - AMB_RS01285 0.45 -0.2 284,792 - AMB_RS01285 0.45 -1.3 284,978 + AMB_RS01285 0.65 -1.0 284,979 - AMB_RS01285 0.65 -0.3 285,053 + AMB_RS01285 0.73 -0.4 285,053 + AMB_RS01285 0.73 -1.0 285,053 + AMB_RS01285 0.73 -0.7 285,053 + AMB_RS01285 0.73 -0.9 285,053 + AMB_RS01285 0.73 -1.6 285,053 + AMB_RS01285 0.73 -0.7 285,053 + AMB_RS01285 0.73 -1.3 285,053 + AMB_RS01285 0.73 -0.8 285,053 + AMB_RS01285 0.73 -0.5 285,053 + AMB_RS01285 0.73 -1.0 285,053 + AMB_RS01285 0.73 -0.1 285,053 + AMB_RS01285 0.73 -0.9 285,053 + AMB_RS01285 0.73 -1.5 285,053 + AMB_RS01285 0.73 -1.5 285,053 + AMB_RS01285 0.73 -0.6 285,053 + AMB_RS01285 0.73 -0.8 285,053 + AMB_RS01285 0.73 -0.4 285,054 - AMB_RS01285 0.73 -0.2 285,054 - AMB_RS01285 0.73 -0.3 285,054 - AMB_RS01285 0.73 +0.1 285,054 - AMB_RS01285 0.73 -1.0 285,054 - AMB_RS01285 0.73 -1.3 285,054 - AMB_RS01285 0.73 -0.7 285,191 + AMB_RS01285 0.88 -1.7 285,340 + -2.5 285,340 + -0.5 285,340 + -2.0 285,341 - +0.4 285,341 - +0.0 285,341 - +0.3 285,341 - +0.0 285,448 + AMB_RS01290 0.16 +0.7 285,448 + AMB_RS01290 0.16 -0.6 285,448 + AMB_RS01290 0.16 -1.1 285,448 + AMB_RS01290 0.16 -2.5 285,448 + AMB_RS01290 0.16 +0.8 285,448 + AMB_RS01290 0.16 -0.2 285,448 + AMB_RS01290 0.16 -1.4 285,449 - AMB_RS01290 0.16 -0.5 285,449 - AMB_RS01290 0.16 -0.3 285,449 - AMB_RS01290 0.16 +1.5 285,449 - AMB_RS01290 0.16 +0.3 285,449 - AMB_RS01290 0.16 -0.3 285,449 - AMB_RS01290 0.16 +0.2 285,449 - AMB_RS01290 0.16 -1.1 285,449 - AMB_RS01290 0.16 +0.0 285,687 + AMB_RS01290 0.42 -0.7 285,687 + AMB_RS01290 0.42 -0.7 285,687 + AMB_RS01290 0.42 -3.3 285,688 - AMB_RS01290 0.42 -0.3 285,688 - AMB_RS01290 0.42 -0.4 285,772 + AMB_RS01290 0.51 -1.5 285,773 - AMB_RS01290 0.51 +0.3 285,846 + AMB_RS01290 0.59 -0.3 285,846 + AMB_RS01290 0.59 -2.4 286,140 + -2.3 286,198 + -0.7 286,198 + -3.4 286,199 - +0.1 286,199 - +0.1 286,199 - -0.7 286,372 - AMB_RS01295 0.15 -0.1 286,561 + AMB_RS01295 0.35 -1.0 286,562 - AMB_RS01295 0.35 -1.1 286,686 + AMB_RS01295 0.48 -0.7 286,686 + AMB_RS01295 0.48 -0.9 286,687 - AMB_RS01295 0.48 +0.5 286,956 + AMB_RS01295 0.76 +0.8 286,957 - AMB_RS01295 0.76 +1.0 286,957 - AMB_RS01295 0.76 -0.3 286,977 + AMB_RS01295 0.78 -0.7 286,977 + AMB_RS01295 0.78 +0.5 286,977 + AMB_RS01295 0.78 -1.8 286,977 + AMB_RS01295 0.78 -0.3 286,977 + AMB_RS01295 0.78 -0.1 286,977 + AMB_RS01295 0.78 -1.1 286,978 - AMB_RS01295 0.78 +0.1 286,978 - AMB_RS01295 0.78 +0.1 286,978 - AMB_RS01295 0.78 -0.4 286,978 - AMB_RS01295 0.78 +0.3 286,978 - AMB_RS01295 0.78 +0.3 286,978 - AMB_RS01295 0.78 -0.3 286,978 - AMB_RS01295 0.78 -0.5 286,978 - AMB_RS01295 0.78 -2.5 286,978 - AMB_RS01295 0.78 +0.0 286,978 - AMB_RS01295 0.78 -0.6 286,990 - AMB_RS01295 0.79 -0.5 287,143 - -1.0 287,215 + -0.3 287,215 + -1.0 287,216 - -0.7
Or see this region's nucleotide sequence