Experiment: 150_initial_3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01170 and AMB_RS01175 are separated by 23 nucleotides AMB_RS01175 and AMB_RS01180 overlap by 16 nucleotides AMB_RS01180 and AMB_RS01185 are separated by 3 nucleotides
AMB_RS01170: AMB_RS01170 - copper export protein, at 259,328 to 260,911
_RS01170
AMB_RS01175: AMB_RS01175 - C4-dicarboxylate ABC transporter, at 260,935 to 262,242
_RS01175
AMB_RS01180: AMB_RS01180 - dihydrofolate reductase, at 262,227 to 262,748
_RS01180
AMB_RS01185: AMB_RS01185 - HD domain-containing protein, at 262,752 to 264,527
_RS01185
Position (kb) 260
261
262
263 Strain fitness (log2 ratio) -2
-1
0
1 at 259.982 kb on + strand, within AMB_RS01170 at 259.982 kb on + strand, within AMB_RS01170 at 259.983 kb on - strand, within AMB_RS01170 at 260.019 kb on - strand, within AMB_RS01170 at 260.758 kb on + strand at 260.759 kb on - strand at 260.952 kb on + strand at 260.952 kb on + strand at 260.952 kb on + strand at 261.019 kb on + strand at 261.019 kb on + strand at 261.019 kb on + strand at 261.019 kb on + strand at 261.020 kb on - strand at 261.020 kb on - strand at 261.334 kb on - strand, within AMB_RS01175 at 261.395 kb on - strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.871 kb on + strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.872 kb on - strand, within AMB_RS01175 at 261.935 kb on - strand, within AMB_RS01175 at 261.973 kb on + strand, within AMB_RS01175 at 261.973 kb on + strand, within AMB_RS01175 at 261.974 kb on - strand, within AMB_RS01175 at 262.012 kb on - strand, within AMB_RS01175 at 262.181 kb on - strand at 262.181 kb on - strand at 262.240 kb on + strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.241 kb on - strand at 262.265 kb on + strand at 262.266 kb on - strand at 262.266 kb on - strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.267 kb on + strand at 262.268 kb on - strand at 262.268 kb on - strand at 262.268 kb on - strand at 262.293 kb on - strand, within AMB_RS01180 at 262.588 kb on + strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.589 kb on - strand, within AMB_RS01180 at 262.702 kb on + strand at 262.703 kb on - strand at 262.726 kb on + strand at 262.727 kb on - strand at 262.727 kb on - strand at 262.727 kb on - strand at 262.774 kb on + strand at 262.775 kb on - strand at 262.775 kb on - strand at 262.817 kb on - strand at 262.946 kb on - strand, within AMB_RS01185 at 262.946 kb on - strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.135 kb on + strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.136 kb on - strand, within AMB_RS01185 at 263.209 kb on + strand, within AMB_RS01185 at 263.209 kb on + strand, within AMB_RS01185 at 263.210 kb on - strand, within AMB_RS01185 at 263.227 kb on + strand, within AMB_RS01185
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_3 remove 259,982 + AMB_RS01170 0.41 +0.9 259,982 + AMB_RS01170 0.41 +0.1 259,983 - AMB_RS01170 0.41 +1.2 260,019 - AMB_RS01170 0.44 +1.5 260,758 + -0.3 260,759 - -0.4 260,952 + +0.4 260,952 + -0.7 260,952 + +0.1 261,019 + -0.4 261,019 + -0.1 261,019 + -0.5 261,019 + +0.7 261,020 - +0.3 261,020 - -0.3 261,334 - AMB_RS01175 0.31 -0.2 261,395 - AMB_RS01175 0.35 -0.5 261,871 + AMB_RS01175 0.72 +1.1 261,871 + AMB_RS01175 0.72 -0.3 261,871 + AMB_RS01175 0.72 -0.7 261,871 + AMB_RS01175 0.72 -0.8 261,871 + AMB_RS01175 0.72 -0.4 261,871 + AMB_RS01175 0.72 -0.2 261,872 - AMB_RS01175 0.72 -1.0 261,872 - AMB_RS01175 0.72 +0.4 261,872 - AMB_RS01175 0.72 -0.2 261,935 - AMB_RS01175 0.76 +0.3 261,973 + AMB_RS01175 0.79 +0.4 261,973 + AMB_RS01175 0.79 -0.2 261,974 - AMB_RS01175 0.79 +0.1 262,012 - AMB_RS01175 0.82 -1.8 262,181 - +0.5 262,181 - -0.2 262,240 + -1.3 262,241 - +0.2 262,241 - -0.7 262,241 - +1.0 262,241 - -0.1 262,241 - -0.3 262,265 + +0.2 262,266 - -0.5 262,266 - -0.1 262,267 + -0.8 262,267 + +0.2 262,267 + -0.7 262,267 + -1.0 262,267 + +0.2 262,268 - -0.5 262,268 - +0.3 262,268 - +0.0 262,293 - AMB_RS01180 0.13 +0.4 262,588 + AMB_RS01180 0.69 +0.8 262,589 - AMB_RS01180 0.69 +0.4 262,589 - AMB_RS01180 0.69 +1.2 262,589 - AMB_RS01180 0.69 -1.1 262,589 - AMB_RS01180 0.69 +0.4 262,589 - AMB_RS01180 0.69 -1.0 262,702 + -0.1 262,703 - +0.7 262,726 + +0.4 262,727 - -0.0 262,727 - +0.8 262,727 - -1.9 262,774 + +1.6 262,775 - +1.3 262,775 - +0.6 262,817 - -0.7 262,946 - AMB_RS01185 0.11 -0.3 262,946 - AMB_RS01185 0.11 +1.0 263,135 + AMB_RS01185 0.22 +0.4 263,135 + AMB_RS01185 0.22 +0.4 263,135 + AMB_RS01185 0.22 +0.0 263,136 - AMB_RS01185 0.22 +0.8 263,136 - AMB_RS01185 0.22 -1.3 263,136 - AMB_RS01185 0.22 +0.3 263,209 + AMB_RS01185 0.26 +0.9 263,209 + AMB_RS01185 0.26 -0.2 263,210 - AMB_RS01185 0.26 +0.9 263,227 + AMB_RS01185 0.27 -1.2
Or see this region's nucleotide sequence