Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS12220 and ABIE40_RS12225 overlap by 4 nucleotides ABIE40_RS12225 and ABIE40_RS12230 are separated by 52 nucleotides ABIE40_RS12230 and ABIE40_RS12235 are separated by 21 nucleotides
ABIE40_RS12220: ABIE40_RS12220 - nitrile hydratase subunit alpha, at 2,380,822 to 2,381,493
_RS12220
ABIE40_RS12225: ABIE40_RS12225 - SH3-like domain-containing protein, at 2,381,490 to 2,381,822
_RS12225
ABIE40_RS12230: ABIE40_RS12230 - carboxymuconolactone decarboxylase family protein, at 2,381,875 to 2,382,216
_RS12230
ABIE40_RS12235: ABIE40_RS12235 - MBL fold metallo-hydrolase, at 2,382,238 to 2,383,155
_RS12235
Position (kb)
2381
2382
2383 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2380.946 kb on + strand, within ABIE40_RS12220 at 2380.946 kb on + strand, within ABIE40_RS12220 at 2380.977 kb on + strand, within ABIE40_RS12220 at 2380.978 kb on - strand, within ABIE40_RS12220 at 2381.036 kb on + strand, within ABIE40_RS12220 at 2381.036 kb on + strand, within ABIE40_RS12220 at 2381.037 kb on - strand, within ABIE40_RS12220 at 2381.070 kb on - strand, within ABIE40_RS12220 at 2381.070 kb on - strand, within ABIE40_RS12220 at 2381.326 kb on + strand, within ABIE40_RS12220 at 2381.326 kb on + strand, within ABIE40_RS12220 at 2381.371 kb on + strand, within ABIE40_RS12220 at 2381.393 kb on + strand, within ABIE40_RS12220 at 2381.393 kb on + strand, within ABIE40_RS12220 at 2381.393 kb on + strand, within ABIE40_RS12220 at 2381.393 kb on + strand, within ABIE40_RS12220 at 2381.393 kb on + strand, within ABIE40_RS12220 at 2381.394 kb on - strand, within ABIE40_RS12220 at 2381.394 kb on - strand, within ABIE40_RS12220 at 2381.397 kb on - strand, within ABIE40_RS12220 at 2381.515 kb on + strand at 2381.515 kb on + strand at 2381.523 kb on + strand at 2381.524 kb on - strand, within ABIE40_RS12225 at 2381.524 kb on - strand, within ABIE40_RS12225 at 2381.524 kb on - strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.552 kb on + strand, within ABIE40_RS12225 at 2381.553 kb on - strand, within ABIE40_RS12225 at 2381.553 kb on - strand, within ABIE40_RS12225 at 2381.553 kb on - strand, within ABIE40_RS12225 at 2381.553 kb on - strand, within ABIE40_RS12225 at 2381.554 kb on + strand, within ABIE40_RS12225 at 2381.554 kb on + strand, within ABIE40_RS12225 at 2381.555 kb on - strand, within ABIE40_RS12225 at 2381.555 kb on - strand, within ABIE40_RS12225 at 2381.555 kb on - strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.593 kb on + strand, within ABIE40_RS12225 at 2381.594 kb on - strand, within ABIE40_RS12225 at 2381.594 kb on - strand, within ABIE40_RS12225 at 2381.596 kb on + strand, within ABIE40_RS12225 at 2381.596 kb on + strand, within ABIE40_RS12225 at 2381.596 kb on + strand, within ABIE40_RS12225 at 2381.597 kb on - strand, within ABIE40_RS12225 at 2381.597 kb on - strand, within ABIE40_RS12225 at 2381.822 kb on + strand at 2381.822 kb on + strand at 2381.822 kb on + strand at 2381.822 kb on + strand at 2381.822 kb on + strand at 2381.823 kb on - strand at 2381.823 kb on - strand at 2381.823 kb on - strand at 2381.823 kb on - strand at 2381.851 kb on - strand at 2381.876 kb on + strand at 2381.876 kb on + strand at 2381.876 kb on + strand at 2382.009 kb on + strand, within ABIE40_RS12230 at 2382.009 kb on + strand, within ABIE40_RS12230 at 2382.009 kb on + strand, within ABIE40_RS12230 at 2382.191 kb on + strand at 2382.191 kb on + strand at 2382.192 kb on - strand at 2382.192 kb on - strand at 2382.192 kb on - strand at 2382.192 kb on - strand at 2382.282 kb on + strand at 2382.282 kb on + strand at 2382.399 kb on + strand, within ABIE40_RS12235 at 2382.399 kb on + strand, within ABIE40_RS12235 at 2382.400 kb on - strand, within ABIE40_RS12235 at 2382.400 kb on - strand, within ABIE40_RS12235 at 2382.400 kb on - strand, within ABIE40_RS12235 at 2382.400 kb on - strand, within ABIE40_RS12235 at 2382.400 kb on - strand, within ABIE40_RS12235 at 2382.468 kb on + strand, within ABIE40_RS12235 at 2382.519 kb on + strand, within ABIE40_RS12235 at 2382.537 kb on - strand, within ABIE40_RS12235 at 2382.537 kb on - strand, within ABIE40_RS12235 at 2382.552 kb on - strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.772 kb on + strand, within ABIE40_RS12235 at 2382.773 kb on - strand, within ABIE40_RS12235 at 2382.773 kb on - strand, within ABIE40_RS12235 at 2382.843 kb on + strand, within ABIE40_RS12235 at 2382.896 kb on - strand, within ABIE40_RS12235 at 2382.988 kb on - strand, within ABIE40_RS12235 at 2383.033 kb on - strand, within ABIE40_RS12235 at 2383.103 kb on - strand at 2383.103 kb on - strand at 2383.103 kb on - strand at 2383.117 kb on - strand at 2383.117 kb on - strand at 2383.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 2,380,946 + ABIE40_RS12220 0.18 +0.7 2,380,946 + ABIE40_RS12220 0.18 -1.9 2,380,977 + ABIE40_RS12220 0.23 +0.5 2,380,978 - ABIE40_RS12220 0.23 +0.1 2,381,036 + ABIE40_RS12220 0.32 -0.7 2,381,036 + ABIE40_RS12220 0.32 -0.2 2,381,037 - ABIE40_RS12220 0.32 +2.0 2,381,070 - ABIE40_RS12220 0.37 -0.9 2,381,070 - ABIE40_RS12220 0.37 +0.7 2,381,326 + ABIE40_RS12220 0.75 -2.0 2,381,326 + ABIE40_RS12220 0.75 +1.4 2,381,371 + ABIE40_RS12220 0.82 -0.7 2,381,393 + ABIE40_RS12220 0.85 -0.5 2,381,393 + ABIE40_RS12220 0.85 -0.3 2,381,393 + ABIE40_RS12220 0.85 -2.1 2,381,393 + ABIE40_RS12220 0.85 -1.6 2,381,393 + ABIE40_RS12220 0.85 -0.3 2,381,394 - ABIE40_RS12220 0.85 -0.4 2,381,394 - ABIE40_RS12220 0.85 -1.4 2,381,397 - ABIE40_RS12220 0.86 +0.1 2,381,515 + -1.2 2,381,515 + +0.6 2,381,523 + -0.5 2,381,524 - ABIE40_RS12225 0.10 -1.3 2,381,524 - ABIE40_RS12225 0.10 -0.5 2,381,524 - ABIE40_RS12225 0.10 -0.0 2,381,552 + ABIE40_RS12225 0.19 +1.5 2,381,552 + ABIE40_RS12225 0.19 -1.7 2,381,552 + ABIE40_RS12225 0.19 +0.8 2,381,552 + ABIE40_RS12225 0.19 -2.8 2,381,552 + ABIE40_RS12225 0.19 +0.8 2,381,552 + ABIE40_RS12225 0.19 +0.3 2,381,553 - ABIE40_RS12225 0.19 +0.3 2,381,553 - ABIE40_RS12225 0.19 +1.1 2,381,553 - ABIE40_RS12225 0.19 -0.8 2,381,553 - ABIE40_RS12225 0.19 +0.4 2,381,554 + ABIE40_RS12225 0.19 +0.9 2,381,554 + ABIE40_RS12225 0.19 +0.3 2,381,555 - ABIE40_RS12225 0.20 -0.6 2,381,555 - ABIE40_RS12225 0.20 +0.3 2,381,555 - ABIE40_RS12225 0.20 -1.7 2,381,593 + ABIE40_RS12225 0.31 +0.4 2,381,593 + ABIE40_RS12225 0.31 +1.7 2,381,593 + ABIE40_RS12225 0.31 -0.2 2,381,593 + ABIE40_RS12225 0.31 +0.9 2,381,593 + ABIE40_RS12225 0.31 +0.5 2,381,593 + ABIE40_RS12225 0.31 +0.1 2,381,593 + ABIE40_RS12225 0.31 -1.1 2,381,593 + ABIE40_RS12225 0.31 +0.5 2,381,594 - ABIE40_RS12225 0.31 -0.6 2,381,594 - ABIE40_RS12225 0.31 -0.7 2,381,596 + ABIE40_RS12225 0.32 +0.1 2,381,596 + ABIE40_RS12225 0.32 -3.0 2,381,596 + ABIE40_RS12225 0.32 -0.7 2,381,597 - ABIE40_RS12225 0.32 -0.0 2,381,597 - ABIE40_RS12225 0.32 -0.3 2,381,822 + -0.8 2,381,822 + +0.3 2,381,822 + -0.6 2,381,822 + +1.1 2,381,822 + -0.8 2,381,823 - -0.9 2,381,823 - -1.2 2,381,823 - +0.2 2,381,823 - +1.1 2,381,851 - +0.7 2,381,876 + -0.3 2,381,876 + +0.6 2,381,876 + +0.5 2,382,009 + ABIE40_RS12230 0.39 -2.8 2,382,009 + ABIE40_RS12230 0.39 -1.8 2,382,009 + ABIE40_RS12230 0.39 -0.2 2,382,191 + +0.6 2,382,191 + +0.3 2,382,192 - -1.3 2,382,192 - +0.1 2,382,192 - -0.3 2,382,192 - -3.0 2,382,282 + -1.3 2,382,282 + -0.1 2,382,399 + ABIE40_RS12235 0.18 +3.2 2,382,399 + ABIE40_RS12235 0.18 -0.2 2,382,400 - ABIE40_RS12235 0.18 -0.3 2,382,400 - ABIE40_RS12235 0.18 +0.9 2,382,400 - ABIE40_RS12235 0.18 -1.8 2,382,400 - ABIE40_RS12235 0.18 +0.1 2,382,400 - ABIE40_RS12235 0.18 -0.4 2,382,468 + ABIE40_RS12235 0.25 -0.2 2,382,519 + ABIE40_RS12235 0.31 +0.2 2,382,537 - ABIE40_RS12235 0.33 -1.0 2,382,537 - ABIE40_RS12235 0.33 +0.5 2,382,552 - ABIE40_RS12235 0.34 -0.2 2,382,772 + ABIE40_RS12235 0.58 +1.7 2,382,772 + ABIE40_RS12235 0.58 -1.3 2,382,772 + ABIE40_RS12235 0.58 -1.8 2,382,772 + ABIE40_RS12235 0.58 +0.1 2,382,772 + ABIE40_RS12235 0.58 +0.5 2,382,772 + ABIE40_RS12235 0.58 +0.8 2,382,772 + ABIE40_RS12235 0.58 -0.9 2,382,772 + ABIE40_RS12235 0.58 +0.1 2,382,772 + ABIE40_RS12235 0.58 -2.9 2,382,773 - ABIE40_RS12235 0.58 -1.7 2,382,773 - ABIE40_RS12235 0.58 -0.5 2,382,843 + ABIE40_RS12235 0.66 -1.4 2,382,896 - ABIE40_RS12235 0.72 +0.5 2,382,988 - ABIE40_RS12235 0.82 -2.0 2,383,033 - ABIE40_RS12235 0.87 +0.6 2,383,103 - +0.2 2,383,103 - -3.1 2,383,103 - -0.7 2,383,117 - -0.9 2,383,117 - -0.1 2,383,183 + +1.0
Or see this region's nucleotide sequence