Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS01635 and ABIE40_RS01640 overlap by 4 nucleotides ABIE40_RS01640 and ABIE40_RS01645 are separated by 180 nucleotides ABIE40_RS01645 and ABIE40_RS01650 are separated by 100 nucleotides ABIE40_RS01650 and ABIE40_RS01655 are separated by 50 nucleotides
ABIE40_RS01635: ABIE40_RS01635 - VOC family protein, at 323,619 to 324,035
_RS01635
ABIE40_RS01640: ABIE40_RS01640 - ribonuclease PH, at 324,032 to 324,751
_RS01640
ABIE40_RS01645: ABIE40_RS01645 - heat-inducible transcriptional repressor HrcA, at 324,932 to 326,023
_RS01645
ABIE40_RS01650: ABIE40_RS01650 - nucleotide exchange factor GrpE, at 326,124 to 326,756
_RS01650
ABIE40_RS01655: ABIE40_RS01655 - nucleoside hydrolase, at 326,807 to 327,751
_RS01655
Position (kb) 324
325
326
327 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 323.934 kb on - strand, within ABIE40_RS01635 at 323.934 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.963 kb on - strand, within ABIE40_RS01635 at 323.996 kb on - strand at 323.996 kb on - strand at 324.220 kb on - strand, within ABIE40_RS01640 at 324.340 kb on - strand, within ABIE40_RS01640 at 324.340 kb on - strand, within ABIE40_RS01640 at 324.565 kb on - strand, within ABIE40_RS01640 at 324.565 kb on - strand, within ABIE40_RS01640 at 324.803 kb on - strand at 324.803 kb on - strand at 324.836 kb on - strand at 324.836 kb on - strand at 324.890 kb on + strand at 324.890 kb on + strand at 324.891 kb on - strand at 324.892 kb on + strand at 324.893 kb on - strand at 324.893 kb on - strand at 324.925 kb on - strand at 324.925 kb on - strand at 325.019 kb on + strand at 325.020 kb on - strand at 325.020 kb on - strand at 325.020 kb on - strand at 325.136 kb on + strand, within ABIE40_RS01645 at 325.136 kb on + strand, within ABIE40_RS01645 at 325.136 kb on + strand, within ABIE40_RS01645 at 325.136 kb on + strand, within ABIE40_RS01645 at 325.136 kb on + strand, within ABIE40_RS01645 at 325.137 kb on - strand, within ABIE40_RS01645 at 325.137 kb on - strand, within ABIE40_RS01645 at 325.137 kb on - strand, within ABIE40_RS01645 at 325.351 kb on + strand, within ABIE40_RS01645 at 325.525 kb on + strand, within ABIE40_RS01645 at 325.525 kb on + strand, within ABIE40_RS01645 at 325.525 kb on + strand, within ABIE40_RS01645 at 325.526 kb on - strand, within ABIE40_RS01645 at 325.526 kb on - strand, within ABIE40_RS01645 at 325.781 kb on - strand, within ABIE40_RS01645 at 325.949 kb on + strand at 325.949 kb on + strand at 325.949 kb on + strand at 325.949 kb on + strand at 325.950 kb on - strand at 325.950 kb on - strand at 325.976 kb on + strand at 326.734 kb on - strand at 326.754 kb on + strand at 326.754 kb on + strand at 326.755 kb on - strand at 326.755 kb on - strand at 326.981 kb on + strand, within ABIE40_RS01655 at 326.981 kb on + strand, within ABIE40_RS01655 at 326.981 kb on + strand, within ABIE40_RS01655 at 326.981 kb on + strand, within ABIE40_RS01655 at 326.981 kb on + strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 326.982 kb on - strand, within ABIE40_RS01655 at 327.000 kb on + strand, within ABIE40_RS01655 at 327.001 kb on - strand, within ABIE40_RS01655 at 327.001 kb on - strand, within ABIE40_RS01655
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 323,934 - ABIE40_RS01635 0.76 +0.3 323,934 - ABIE40_RS01635 0.76 -2.5 323,963 - ABIE40_RS01635 0.82 -0.1 323,963 - ABIE40_RS01635 0.82 -0.4 323,963 - ABIE40_RS01635 0.82 -1.8 323,963 - ABIE40_RS01635 0.82 -1.3 323,963 - ABIE40_RS01635 0.82 -0.4 323,963 - ABIE40_RS01635 0.82 +0.1 323,996 - -3.1 323,996 - +0.2 324,220 - ABIE40_RS01640 0.26 -0.3 324,340 - ABIE40_RS01640 0.43 -2.0 324,340 - ABIE40_RS01640 0.43 -0.1 324,565 - ABIE40_RS01640 0.74 +0.6 324,565 - ABIE40_RS01640 0.74 -0.5 324,803 - +2.8 324,803 - -0.4 324,836 - -0.1 324,836 - +0.1 324,890 + -2.4 324,890 + -0.6 324,891 - -1.9 324,892 + -0.7 324,893 - -2.3 324,893 - -0.3 324,925 - -0.4 324,925 - +0.4 325,019 + -1.2 325,020 - -1.9 325,020 - +0.7 325,020 - +2.0 325,136 + ABIE40_RS01645 0.19 -2.2 325,136 + ABIE40_RS01645 0.19 -1.1 325,136 + ABIE40_RS01645 0.19 -3.3 325,136 + ABIE40_RS01645 0.19 -1.9 325,136 + ABIE40_RS01645 0.19 -2.7 325,137 - ABIE40_RS01645 0.19 -1.7 325,137 - ABIE40_RS01645 0.19 -1.4 325,137 - ABIE40_RS01645 0.19 -2.6 325,351 + ABIE40_RS01645 0.38 -1.0 325,525 + ABIE40_RS01645 0.54 -0.1 325,525 + ABIE40_RS01645 0.54 -2.8 325,525 + ABIE40_RS01645 0.54 -0.7 325,526 - ABIE40_RS01645 0.54 -3.1 325,526 - ABIE40_RS01645 0.54 -1.4 325,781 - ABIE40_RS01645 0.78 -0.3 325,949 + -0.6 325,949 + -1.8 325,949 + +0.0 325,949 + +3.6 325,950 - -1.9 325,950 - -2.7 325,976 + -0.5 326,734 - +0.4 326,754 + +0.5 326,754 + +0.0 326,755 - -0.3 326,755 - -0.3 326,981 + ABIE40_RS01655 0.18 -0.8 326,981 + ABIE40_RS01655 0.18 +1.0 326,981 + ABIE40_RS01655 0.18 -2.3 326,981 + ABIE40_RS01655 0.18 -0.6 326,981 + ABIE40_RS01655 0.18 -0.9 326,982 - ABIE40_RS01655 0.19 +0.2 326,982 - ABIE40_RS01655 0.19 -1.1 326,982 - ABIE40_RS01655 0.19 +0.2 326,982 - ABIE40_RS01655 0.19 -0.1 326,982 - ABIE40_RS01655 0.19 -0.1 326,982 - ABIE40_RS01655 0.19 +2.0 326,982 - ABIE40_RS01655 0.19 +1.0 326,982 - ABIE40_RS01655 0.19 -0.1 326,982 - ABIE40_RS01655 0.19 +0.2 326,982 - ABIE40_RS01655 0.19 +0.3 327,000 + ABIE40_RS01655 0.20 +0.0 327,001 - ABIE40_RS01655 0.21 +1.0 327,001 - ABIE40_RS01655 0.21 -1.1
Or see this region's nucleotide sequence