Strain Fitness in Escherichia coli BW25113 around b1013

Experiment: casaminos (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1012 and ycdC are separated by 230 nucleotidesycdC and putA are separated by 39 nucleotides b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234 b1012 b1013: ycdC - predicted DNA-binding transcriptional regulator (NCBI), at 1,073,465 to 1,074,103 ycdC b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105 putA Position (kb) 1073 1074 1075Strain fitness (log2 ratio) -2 -1 0 1at 1072.477 kb on - strand, within b1012at 1072.477 kb on - strand, within b1012at 1072.730 kb on - strand, within b1012at 1072.755 kb on - strand, within b1012at 1073.238 kb on + strandat 1073.585 kb on - strand, within ycdCat 1073.585 kb on - strand, within ycdCat 1073.819 kb on - strand, within ycdCat 1073.908 kb on + strand, within ycdCat 1074.009 kb on - strand, within ycdCat 1074.073 kb on + strandat 1074.086 kb on - strandat 1074.088 kb on + strandat 1074.088 kb on + strandat 1074.126 kb on - strandat 1074.140 kb on + strandat 1074.195 kb on - strandat 1074.215 kb on - strandat 1074.218 kb on - strandat 1074.218 kb on - strandat 1074.294 kb on + strandat 1074.306 kb on + strandat 1074.438 kb on + strandat 1074.438 kb on + strandat 1074.499 kb on + strandat 1074.529 kb on - strandat 1074.529 kb on - strandat 1074.602 kb on - strand, within putAat 1074.714 kb on + strand, within putAat 1074.790 kb on + strand, within putAat 1074.794 kb on + strand, within putAat 1074.794 kb on + strand, within putAat 1074.847 kb on + strand, within putAat 1074.885 kb on + strand, within putAat 1074.885 kb on + strand, within putAat 1074.904 kb on + strand, within putAat 1074.904 kb on + strand, within putAat 1075.016 kb on + strand, within putAat 1075.031 kb on + strand, within putA

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Per-strain Table

Position Strand Gene LocusTag Fraction casaminos (C)
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1,072,477 - b1012 b1012 0.34 -0.1
1,072,477 - b1012 b1012 0.34 -0.2
1,072,730 - b1012 b1012 0.56 +0.2
1,072,755 - b1012 b1012 0.58 -0.2
1,073,238 + +0.7
1,073,585 - ycdC b1013 0.19 +1.2
1,073,585 - ycdC b1013 0.19 +0.7
1,073,819 - ycdC b1013 0.55 -0.1
1,073,908 + ycdC b1013 0.69 +1.5
1,074,009 - ycdC b1013 0.85 +0.7
1,074,073 + +0.7
1,074,086 - +0.3
1,074,088 + -1.3
1,074,088 + -0.1
1,074,126 - +1.1
1,074,140 + -1.1
1,074,195 - -1.1
1,074,215 - -2.2
1,074,218 - -1.4
1,074,218 - -0.9
1,074,294 + -0.0
1,074,306 + -0.5
1,074,438 + -0.4
1,074,438 + -0.2
1,074,499 + -0.9
1,074,529 - -0.2
1,074,529 - -2.2
1,074,602 - putA b1014 0.12 -1.0
1,074,714 + putA b1014 0.14 -2.5
1,074,790 + putA b1014 0.16 -1.0
1,074,794 + putA b1014 0.16 -0.9
1,074,794 + putA b1014 0.16 -0.6
1,074,847 + putA b1014 0.18 -0.8
1,074,885 + putA b1014 0.19 +0.7
1,074,885 + putA b1014 0.19 -0.3
1,074,904 + putA b1014 0.19 -0.4
1,074,904 + putA b1014 0.19 -1.1
1,075,016 + putA b1014 0.22 -1.6
1,075,031 + putA b1014 0.22 -0.9

Or see this region's nucleotide sequence