Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4471 and EX28DRAFT_4472 are separated by 2 nucleotides EX28DRAFT_4472 and EX28DRAFT_4473 are separated by 20 nucleotides EX28DRAFT_4473 and EX28DRAFT_4474 overlap by 4 nucleotides
EX28DRAFT_4471: EX28DRAFT_4471 - heme biosynthesis-associated TPR protein, at 35,646 to 36,845
_4471
EX28DRAFT_4472: EX28DRAFT_4472 - Uncharacterized enzyme of heme biosynthesis, at 36,848 to 38,044
_4472
EX28DRAFT_4473: EX28DRAFT_4473 - Uroporphyrinogen-III synthase, at 38,065 to 38,805
_4473
EX28DRAFT_4474: EX28DRAFT_4474 - hydroxymethylbilane synthase (EC 2.5.1.61), at 38,802 to 39,764
_4474
Position (kb) 36
37
38
39 Strain fitness (log2 ratio) -2
-1
0
1 at 36.074 kb on + strand, within EX28DRAFT_4471 at 36.256 kb on + strand, within EX28DRAFT_4471 at 36.256 kb on + strand, within EX28DRAFT_4471 at 36.256 kb on + strand, within EX28DRAFT_4471 at 36.257 kb on - strand, within EX28DRAFT_4471 at 36.566 kb on + strand, within EX28DRAFT_4471 at 36.789 kb on - strand at 36.850 kb on - strand at 36.947 kb on + strand at 36.948 kb on - strand at 37.033 kb on + strand, within EX28DRAFT_4472 at 37.039 kb on + strand, within EX28DRAFT_4472 at 37.080 kb on + strand, within EX28DRAFT_4472 at 37.125 kb on + strand, within EX28DRAFT_4472 at 37.125 kb on + strand, within EX28DRAFT_4472 at 37.126 kb on - strand, within EX28DRAFT_4472 at 37.197 kb on + strand, within EX28DRAFT_4472 at 37.197 kb on + strand, within EX28DRAFT_4472 at 37.197 kb on + strand, within EX28DRAFT_4472 at 37.197 kb on + strand, within EX28DRAFT_4472 at 37.198 kb on - strand, within EX28DRAFT_4472 at 37.202 kb on - strand, within EX28DRAFT_4472 at 37.589 kb on - strand, within EX28DRAFT_4472 at 38.067 kb on - strand at 38.072 kb on - strand at 38.800 kb on - strand at 38.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 36,074 + EX28DRAFT_4471 0.36 +0.4 36,256 + EX28DRAFT_4471 0.51 -0.8 36,256 + EX28DRAFT_4471 0.51 -0.1 36,256 + EX28DRAFT_4471 0.51 +0.1 36,257 - EX28DRAFT_4471 0.51 -0.7 36,566 + EX28DRAFT_4471 0.77 -0.3 36,789 - -0.5 36,850 - -0.1 36,947 + -1.1 36,948 - -1.8 37,033 + EX28DRAFT_4472 0.15 -0.6 37,039 + EX28DRAFT_4472 0.16 -0.3 37,080 + EX28DRAFT_4472 0.19 -0.9 37,125 + EX28DRAFT_4472 0.23 -0.1 37,125 + EX28DRAFT_4472 0.23 -0.2 37,126 - EX28DRAFT_4472 0.23 -0.5 37,197 + EX28DRAFT_4472 0.29 +1.0 37,197 + EX28DRAFT_4472 0.29 +1.5 37,197 + EX28DRAFT_4472 0.29 -1.1 37,197 + EX28DRAFT_4472 0.29 -0.8 37,198 - EX28DRAFT_4472 0.29 -2.5 37,202 - EX28DRAFT_4472 0.30 -0.7 37,589 - EX28DRAFT_4472 0.62 -0.5 38,067 - +0.0 38,072 - +1.1 38,800 - -0.2 38,823 - -0.8
Or see this region's nucleotide sequence