Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4457 and EX28DRAFT_4458 overlap by 4 nucleotides EX28DRAFT_4458 and EX28DRAFT_4459 are separated by 4 nucleotides EX28DRAFT_4459 and EX28DRAFT_4460 are separated by 1 nucleotides
EX28DRAFT_4457: EX28DRAFT_4457 - nucleotide sugar dehydrogenase, at 24,117 to 25,379
_4457
EX28DRAFT_4458: EX28DRAFT_4458 - TDP-D-fucosamine acetyltransferase, at 25,376 to 26,053
_4458
EX28DRAFT_4459: EX28DRAFT_4459 - TDP-4-keto-6-deoxy-D-glucose transaminase, at 26,058 to 27,188
_4459
EX28DRAFT_4460: EX28DRAFT_4460 - Membrane protein involved in the export of O-antigen and teichoic acid, at 27,190 to 28,440
_4460
Position (kb) 26
27
28 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 25.297 kb on + strand at 25.347 kb on + strand at 25.347 kb on + strand at 25.348 kb on - strand at 25.349 kb on + strand at 25.350 kb on - strand at 26.046 kb on - strand at 26.103 kb on + strand at 26.103 kb on + strand at 26.104 kb on - strand at 26.105 kb on + strand at 26.106 kb on - strand at 26.106 kb on - strand at 26.293 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459 at 26.440 kb on - strand, within EX28DRAFT_4459 at 26.691 kb on - strand, within EX28DRAFT_4459 at 26.719 kb on + strand, within EX28DRAFT_4459 at 26.723 kb on + strand, within EX28DRAFT_4459 at 26.727 kb on + strand, within EX28DRAFT_4459 at 26.728 kb on - strand, within EX28DRAFT_4459 at 26.781 kb on + strand, within EX28DRAFT_4459 at 26.850 kb on + strand, within EX28DRAFT_4459 at 26.885 kb on + strand, within EX28DRAFT_4459 at 26.886 kb on - strand, within EX28DRAFT_4459 at 26.930 kb on + strand, within EX28DRAFT_4459 at 26.931 kb on - strand, within EX28DRAFT_4459 at 27.161 kb on + strand at 27.189 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 25,297 + -0.3 25,347 + -0.6 25,347 + -3.8 25,348 - -0.3 25,349 + -0.5 25,350 - -0.7 26,046 - +0.0 26,103 + -0.5 26,103 + -0.5 26,104 - -1.2 26,105 + -0.6 26,106 - -1.5 26,106 - -0.5 26,293 - EX28DRAFT_4459 0.21 +0.5 26,295 - EX28DRAFT_4459 0.21 -0.5 26,295 - EX28DRAFT_4459 0.21 -0.3 26,295 - EX28DRAFT_4459 0.21 -1.4 26,440 - EX28DRAFT_4459 0.34 +0.2 26,691 - EX28DRAFT_4459 0.56 -1.0 26,719 + EX28DRAFT_4459 0.58 +0.3 26,723 + EX28DRAFT_4459 0.59 -1.4 26,727 + EX28DRAFT_4459 0.59 -1.4 26,728 - EX28DRAFT_4459 0.59 -1.3 26,781 + EX28DRAFT_4459 0.64 -2.8 26,850 + EX28DRAFT_4459 0.70 +0.4 26,885 + EX28DRAFT_4459 0.73 -2.5 26,886 - EX28DRAFT_4459 0.73 -0.6 26,930 + EX28DRAFT_4459 0.77 -1.5 26,931 - EX28DRAFT_4459 0.77 -0.5 27,161 + +0.5 27,189 + +0.1
Or see this region's nucleotide sequence