Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4456 and EX28DRAFT_4457 overlap by 4 nucleotides EX28DRAFT_4457 and EX28DRAFT_4458 overlap by 4 nucleotides EX28DRAFT_4458 and EX28DRAFT_4459 are separated by 4 nucleotides
EX28DRAFT_4456: EX28DRAFT_4456 - UDP-N-acetylglucosamine 2-epimerase, at 22,984 to 24,120
_4456
EX28DRAFT_4457: EX28DRAFT_4457 - nucleotide sugar dehydrogenase, at 24,117 to 25,379
_4457
EX28DRAFT_4458: EX28DRAFT_4458 - TDP-D-fucosamine acetyltransferase, at 25,376 to 26,053
_4458
EX28DRAFT_4459: EX28DRAFT_4459 - TDP-4-keto-6-deoxy-D-glucose transaminase, at 26,058 to 27,188
_4459
Position (kb) 24
25
26 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 23.155 kb on + strand, within EX28DRAFT_4456 at 23.163 kb on + strand, within EX28DRAFT_4456 at 23.164 kb on - strand, within EX28DRAFT_4456 at 23.166 kb on + strand, within EX28DRAFT_4456 at 23.167 kb on - strand, within EX28DRAFT_4456 at 23.167 kb on - strand, within EX28DRAFT_4456 at 23.265 kb on - strand, within EX28DRAFT_4456 at 23.503 kb on - strand, within EX28DRAFT_4456 at 23.806 kb on + strand, within EX28DRAFT_4456 at 24.049 kb on - strand at 24.096 kb on + strand at 24.153 kb on + strand at 24.154 kb on - strand at 24.154 kb on - strand at 24.233 kb on - strand at 24.535 kb on + strand, within EX28DRAFT_4457 at 24.535 kb on + strand, within EX28DRAFT_4457 at 24.849 kb on - strand, within EX28DRAFT_4457 at 25.025 kb on + strand, within EX28DRAFT_4457 at 25.297 kb on + strand at 25.347 kb on + strand at 25.347 kb on + strand at 25.348 kb on - strand at 25.349 kb on + strand at 25.350 kb on - strand at 26.046 kb on - strand at 26.103 kb on + strand at 26.103 kb on + strand at 26.104 kb on - strand at 26.105 kb on + strand at 26.106 kb on - strand at 26.106 kb on - strand at 26.293 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459 at 26.295 kb on - strand, within EX28DRAFT_4459
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 23,155 + EX28DRAFT_4456 0.15 -0.1 23,163 + EX28DRAFT_4456 0.16 -1.7 23,164 - EX28DRAFT_4456 0.16 +0.2 23,166 + EX28DRAFT_4456 0.16 -1.0 23,167 - EX28DRAFT_4456 0.16 -0.7 23,167 - EX28DRAFT_4456 0.16 -0.9 23,265 - EX28DRAFT_4456 0.25 +0.1 23,503 - EX28DRAFT_4456 0.46 -0.5 23,806 + EX28DRAFT_4456 0.72 +0.2 24,049 - -0.4 24,096 + -1.5 24,153 + -0.8 24,154 - -0.9 24,154 - +0.3 24,233 - -0.6 24,535 + EX28DRAFT_4457 0.33 -3.0 24,535 + EX28DRAFT_4457 0.33 -0.4 24,849 - EX28DRAFT_4457 0.58 -1.0 25,025 + EX28DRAFT_4457 0.72 -1.1 25,297 + -0.3 25,347 + -0.6 25,347 + -3.8 25,348 - -0.3 25,349 + -0.5 25,350 - -0.7 26,046 - +0.0 26,103 + -0.5 26,103 + -0.5 26,104 - -1.2 26,105 + -0.6 26,106 - -1.5 26,106 - -0.5 26,293 - EX28DRAFT_4459 0.21 +0.5 26,295 - EX28DRAFT_4459 0.21 -0.5 26,295 - EX28DRAFT_4459 0.21 -0.3 26,295 - EX28DRAFT_4459 0.21 -1.4
Or see this region's nucleotide sequence