Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4379 and EX28DRAFT_4380 are separated by 114 nucleotides EX28DRAFT_4380 and EX28DRAFT_4381 are separated by 39 nucleotides EX28DRAFT_4381 and EX28DRAFT_4382 overlap by 1 nucleotides
EX28DRAFT_4379: EX28DRAFT_4379 - formate dehydrogenase family accessory protein FdhD, at 44,585 to 45,421
_4379
EX28DRAFT_4380: EX28DRAFT_4380 - Protein of unknown function (DUF1471), at 45,536 to 45,748
_4380
EX28DRAFT_4381: EX28DRAFT_4381 - Branched-chain amino acid transport protein (AzlD), at 45,788 to 46,111
_4381
EX28DRAFT_4382: EX28DRAFT_4382 - Predicted branched-chain amino acid permease (azaleucine resistance), at 46,111 to 46,770
_4382
Position (kb) 45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 44.547 kb on + strand at 44.635 kb on - strand at 44.828 kb on + strand, within EX28DRAFT_4379 at 44.829 kb on - strand, within EX28DRAFT_4379 at 44.829 kb on - strand, within EX28DRAFT_4379 at 45.060 kb on + strand, within EX28DRAFT_4379 at 45.060 kb on + strand, within EX28DRAFT_4379 at 45.245 kb on + strand, within EX28DRAFT_4379 at 45.246 kb on - strand, within EX28DRAFT_4379 at 45.386 kb on + strand at 45.386 kb on + strand at 45.386 kb on + strand at 45.386 kb on + strand at 45.455 kb on - strand at 45.475 kb on - strand at 45.477 kb on + strand at 45.477 kb on + strand at 45.477 kb on + strand at 45.646 kb on + strand, within EX28DRAFT_4380 at 45.742 kb on + strand at 45.794 kb on + strand at 45.795 kb on - strand at 45.821 kb on + strand, within EX28DRAFT_4381 at 45.852 kb on - strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.056 kb on + strand, within EX28DRAFT_4381 at 46.057 kb on - strand, within EX28DRAFT_4381 at 46.276 kb on + strand, within EX28DRAFT_4382 at 46.378 kb on + strand, within EX28DRAFT_4382 at 46.379 kb on - strand, within EX28DRAFT_4382 at 46.379 kb on - strand, within EX28DRAFT_4382 at 46.590 kb on + strand, within EX28DRAFT_4382 at 46.596 kb on + strand, within EX28DRAFT_4382 at 46.633 kb on + strand, within EX28DRAFT_4382 at 46.633 kb on + strand, within EX28DRAFT_4382 at 46.661 kb on + strand, within EX28DRAFT_4382 at 46.683 kb on - strand, within EX28DRAFT_4382 at 46.733 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 44,547 + -0.2 44,635 - +0.1 44,828 + EX28DRAFT_4379 0.29 -0.9 44,829 - EX28DRAFT_4379 0.29 -1.9 44,829 - EX28DRAFT_4379 0.29 -0.2 45,060 + EX28DRAFT_4379 0.57 -0.1 45,060 + EX28DRAFT_4379 0.57 -0.1 45,245 + EX28DRAFT_4379 0.79 -0.4 45,246 - EX28DRAFT_4379 0.79 -0.0 45,386 + -0.7 45,386 + -0.9 45,386 + +0.2 45,386 + +0.1 45,455 - +0.6 45,475 - +0.6 45,477 + -0.0 45,477 + -0.3 45,477 + -0.4 45,646 + EX28DRAFT_4380 0.52 -0.6 45,742 + -0.2 45,794 + -0.3 45,795 - +0.2 45,821 + EX28DRAFT_4381 0.10 -0.6 45,852 - EX28DRAFT_4381 0.20 -0.3 46,056 + EX28DRAFT_4381 0.83 +0.0 46,056 + EX28DRAFT_4381 0.83 +0.2 46,056 + EX28DRAFT_4381 0.83 -1.8 46,056 + EX28DRAFT_4381 0.83 +0.3 46,057 - EX28DRAFT_4381 0.83 +0.4 46,276 + EX28DRAFT_4382 0.25 -0.3 46,378 + EX28DRAFT_4382 0.40 -0.2 46,379 - EX28DRAFT_4382 0.41 -1.2 46,379 - EX28DRAFT_4382 0.41 +0.4 46,590 + EX28DRAFT_4382 0.73 -0.1 46,596 + EX28DRAFT_4382 0.73 +0.9 46,633 + EX28DRAFT_4382 0.79 +0.4 46,633 + EX28DRAFT_4382 0.79 +1.1 46,661 + EX28DRAFT_4382 0.83 +0.3 46,683 - EX28DRAFT_4382 0.87 +0.2 46,733 - -0.1
Or see this region's nucleotide sequence