Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4298 and EX28DRAFT_4299 are separated by 127 nucleotides EX28DRAFT_4299 and EX28DRAFT_4300 overlap by 17 nucleotides EX28DRAFT_4300 and EX28DRAFT_4301 are separated by 60 nucleotides EX28DRAFT_4301 and EX28DRAFT_4302 are separated by 129 nucleotides
EX28DRAFT_4298: EX28DRAFT_4298 - signal recognition particle-docking protein FtsY, at 101,676 to 103,136
_4298
EX28DRAFT_4299: EX28DRAFT_4299 - 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD, at 103,264 to 103,866
_4299
EX28DRAFT_4300: EX28DRAFT_4300 - Predicted membrane protein, at 103,850 to 104,131
_4300
EX28DRAFT_4301: EX28DRAFT_4301 - Protein of unknown function (DUF2500), at 104,192 to 104,548
_4301
EX28DRAFT_4302: EX28DRAFT_4302 - Predicted membrane protein, at 104,678 to 105,304
_4302
Position (kb) 103
104 Strain fitness (log2 ratio) -1
0
1 at 103.164 kb on - strand at 103.166 kb on - strand at 103.166 kb on - strand at 103.166 kb on - strand at 103.166 kb on - strand at 103.166 kb on - strand at 103.166 kb on - strand at 103.193 kb on - strand at 103.193 kb on - strand at 103.205 kb on + strand at 103.209 kb on + strand at 103.209 kb on + strand at 103.210 kb on - strand at 103.366 kb on - strand, within EX28DRAFT_4299 at 103.395 kb on + strand, within EX28DRAFT_4299 at 103.982 kb on - strand, within EX28DRAFT_4300 at 104.229 kb on + strand, within EX28DRAFT_4301 at 104.229 kb on + strand, within EX28DRAFT_4301 at 104.268 kb on + strand, within EX28DRAFT_4301 at 104.320 kb on + strand, within EX28DRAFT_4301 at 104.583 kb on + strand at 104.626 kb on + strand at 104.627 kb on - strand at 104.674 kb on + strand at 104.674 kb on + strand at 104.678 kb on - strand at 104.678 kb on - strand at 104.692 kb on + strand at 104.693 kb on - strand at 104.720 kb on + strand at 104.721 kb on - strand at 104.768 kb on - strand, within EX28DRAFT_4302 at 104.834 kb on + strand, within EX28DRAFT_4302
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 103,164 - +0.7 103,166 - -0.2 103,166 - -0.3 103,166 - +0.1 103,166 - -0.3 103,166 - +0.4 103,166 - -0.2 103,193 - -0.2 103,193 - -0.2 103,205 + +0.1 103,209 + +0.4 103,209 + -0.3 103,210 - +0.4 103,366 - EX28DRAFT_4299 0.17 -0.4 103,395 + EX28DRAFT_4299 0.22 -1.1 103,982 - EX28DRAFT_4300 0.47 -0.5 104,229 + EX28DRAFT_4301 0.10 -0.1 104,229 + EX28DRAFT_4301 0.10 +0.4 104,268 + EX28DRAFT_4301 0.21 +0.4 104,320 + EX28DRAFT_4301 0.36 -0.7 104,583 + -1.2 104,626 + -0.6 104,627 - -0.3 104,674 + -1.0 104,674 + +0.9 104,678 - +1.0 104,678 - -0.5 104,692 + -0.6 104,693 - -0.7 104,720 + -0.0 104,721 - +1.4 104,768 - EX28DRAFT_4302 0.14 +0.4 104,834 + EX28DRAFT_4302 0.25 -1.6
Or see this region's nucleotide sequence