Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4154 and EX28DRAFT_4155 are separated by 67 nucleotides EX28DRAFT_4155 and EX28DRAFT_4156 are separated by 41 nucleotides EX28DRAFT_4156 and EX28DRAFT_4157 overlap by 8 nucleotides EX28DRAFT_4157 and EX28DRAFT_4158 overlap by 8 nucleotides
EX28DRAFT_4154: EX28DRAFT_4154 - Uncharacterized protein conserved in bacteria, at 135,091 to 136,407
_4154
EX28DRAFT_4155: EX28DRAFT_4155 - DNA adenine methylase (dam), at 136,475 to 137,287
_4155
EX28DRAFT_4156: EX28DRAFT_4156 - ribulose-phosphate 3-epimerase, at 137,329 to 138,006
_4156
EX28DRAFT_4157: EX28DRAFT_4157 - 2-phosphoglycolate phosphatase, prokaryotic, at 137,999 to 138,760
_4157
EX28DRAFT_4158: EX28DRAFT_4158 - tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 138,753 to 139,757
_4158
Position (kb) 137
138
139 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 136.406 kb on - strand at 136.459 kb on + strand at 136.459 kb on + strand at 136.537 kb on + strand at 136.624 kb on + strand, within EX28DRAFT_4155 at 136.847 kb on + strand, within EX28DRAFT_4155 at 136.979 kb on - strand, within EX28DRAFT_4155 at 137.009 kb on + strand, within EX28DRAFT_4155 at 137.171 kb on - strand, within EX28DRAFT_4155 at 137.183 kb on + strand, within EX28DRAFT_4155 at 137.222 kb on - strand at 137.228 kb on - strand at 137.495 kb on + strand, within EX28DRAFT_4156 at 137.499 kb on + strand, within EX28DRAFT_4156 at 137.710 kb on + strand, within EX28DRAFT_4156 at 137.993 kb on + strand at 137.993 kb on + strand at 138.004 kb on + strand at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.139 kb on - strand, within EX28DRAFT_4157 at 138.259 kb on + strand, within EX28DRAFT_4157 at 138.305 kb on + strand, within EX28DRAFT_4157 at 138.458 kb on + strand, within EX28DRAFT_4157 at 138.458 kb on + strand, within EX28DRAFT_4157 at 138.543 kb on - strand, within EX28DRAFT_4157 at 138.589 kb on + strand, within EX28DRAFT_4157 at 138.590 kb on - strand, within EX28DRAFT_4157 at 138.636 kb on - strand, within EX28DRAFT_4157 at 138.636 kb on - strand, within EX28DRAFT_4157 at 138.647 kb on + strand, within EX28DRAFT_4157 at 138.647 kb on + strand, within EX28DRAFT_4157 at 138.666 kb on + strand, within EX28DRAFT_4157 at 138.774 kb on - strand at 138.813 kb on + strand at 138.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 136,406 - -1.0 136,459 + -0.6 136,459 + -0.7 136,537 + -3.0 136,624 + EX28DRAFT_4155 0.18 -2.4 136,847 + EX28DRAFT_4155 0.46 -0.3 136,979 - EX28DRAFT_4155 0.62 +0.3 137,009 + EX28DRAFT_4155 0.66 -0.1 137,171 - EX28DRAFT_4155 0.86 +0.1 137,183 + EX28DRAFT_4155 0.87 -0.3 137,222 - -1.4 137,228 - -2.1 137,495 + EX28DRAFT_4156 0.24 -0.5 137,499 + EX28DRAFT_4156 0.25 -1.3 137,710 + EX28DRAFT_4156 0.56 -0.8 137,993 + +0.9 137,993 + -0.3 138,004 + +0.8 138,099 - EX28DRAFT_4157 0.13 -0.4 138,099 - EX28DRAFT_4157 0.13 -0.7 138,138 + EX28DRAFT_4157 0.18 -0.4 138,138 + EX28DRAFT_4157 0.18 -0.5 138,139 - EX28DRAFT_4157 0.18 +0.1 138,259 + EX28DRAFT_4157 0.34 +0.2 138,305 + EX28DRAFT_4157 0.40 -2.1 138,458 + EX28DRAFT_4157 0.60 +0.4 138,458 + EX28DRAFT_4157 0.60 +0.3 138,543 - EX28DRAFT_4157 0.71 +0.5 138,589 + EX28DRAFT_4157 0.77 -0.2 138,590 - EX28DRAFT_4157 0.78 -0.3 138,636 - EX28DRAFT_4157 0.84 -0.1 138,636 - EX28DRAFT_4157 0.84 -0.5 138,647 + EX28DRAFT_4157 0.85 +0.3 138,647 + EX28DRAFT_4157 0.85 +0.4 138,666 + EX28DRAFT_4157 0.88 +0.1 138,774 - -1.1 138,813 + -0.7 138,814 - +0.5
Or see this region's nucleotide sequence