Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_3899

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_3897 and EX28DRAFT_3898 are separated by 54 nucleotidesEX28DRAFT_3898 and EX28DRAFT_3899 are separated by 19 nucleotidesEX28DRAFT_3899 and EX28DRAFT_3900 are separated by 37 nucleotidesEX28DRAFT_3900 and EX28DRAFT_3901 are separated by 3 nucleotides EX28DRAFT_3897: EX28DRAFT_3897 - guanylate kinase (EC 2.7.4.8), at 105,144 to 105,767 _3897 EX28DRAFT_3898: EX28DRAFT_3898 - DNA-directed RNA polymerase subunit omega (EC 2.7.7.6), at 105,822 to 106,097 _3898 EX28DRAFT_3899: EX28DRAFT_3899 - (p)ppGpp synthetase, RelA/SpoT family, at 106,117 to 108,231 _3899 EX28DRAFT_3900: EX28DRAFT_3900 - SpoU rRNA Methylase family/SpoU, rRNA methylase, C-terminal, at 108,269 to 108,925 _3900 EX28DRAFT_3901: EX28DRAFT_3901 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 108,929 to 111,010 _3901 Position (kb) 106 107 108 109Strain fitness (log2 ratio) -1 0 1at 105.817 kb on + strandat 105.821 kb on + strandat 105.965 kb on + strand, within EX28DRAFT_3898at 107.922 kb on + strand, within EX28DRAFT_3899at 107.923 kb on - strand, within EX28DRAFT_3899at 107.977 kb on - strand, within EX28DRAFT_3899at 108.010 kb on - strand, within EX28DRAFT_3899at 108.018 kb on + strand, within EX28DRAFT_3899at 108.018 kb on + strand, within EX28DRAFT_3899at 108.018 kb on + strand, within EX28DRAFT_3899at 108.171 kb on + strandat 108.230 kb on - strandat 108.230 kb on - strandat 108.251 kb on + strandat 108.259 kb on + strandat 108.319 kb on + strandat 108.319 kb on + strandat 108.319 kb on + strandat 108.320 kb on - strandat 108.320 kb on - strandat 108.711 kb on - strand, within EX28DRAFT_3900at 108.746 kb on + strand, within EX28DRAFT_3900at 108.747 kb on - strand, within EX28DRAFT_3900at 109.109 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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105,817 + +0.2
105,821 + +0.6
105,965 + EX28DRAFT_3898 0.52 -0.1
107,922 + EX28DRAFT_3899 0.85 -0.9
107,923 - EX28DRAFT_3899 0.85 -0.8
107,977 - EX28DRAFT_3899 0.88 -1.3
108,010 - EX28DRAFT_3899 0.90 +0.2
108,018 + EX28DRAFT_3899 0.90 +0.1
108,018 + EX28DRAFT_3899 0.90 -1.0
108,018 + EX28DRAFT_3899 0.90 +0.0
108,171 + +0.2
108,230 - +1.2
108,230 - -0.3
108,251 + -0.2
108,259 + -0.2
108,319 + +0.0
108,319 + +0.1
108,319 + +0.2
108,320 - +0.7
108,320 - -0.4
108,711 - EX28DRAFT_3900 0.67 -0.2
108,746 + EX28DRAFT_3900 0.73 -0.2
108,747 - EX28DRAFT_3900 0.73 +0.5
109,109 + -1.0

Or see this region's nucleotide sequence