Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3424 and EX28DRAFT_3425 are separated by 10 nucleotides EX28DRAFT_3425 and EX28DRAFT_3426 are separated by 2 nucleotides EX28DRAFT_3426 and EX28DRAFT_3427 are separated by 22 nucleotides EX28DRAFT_3427 and EX28DRAFT_3428 are separated by 13 nucleotides
EX28DRAFT_3424: EX28DRAFT_3424 - PRD domain protein EF_0829/AHA_3910, at 95,785 to 96,105
_3424
EX28DRAFT_3425: EX28DRAFT_3425 - conserved hypothetical protein EF_0830/AHA_3911, at 96,116 to 96,478
_3425
EX28DRAFT_3426: EX28DRAFT_3426 - conserved hypothetical protein EF_0831/AHA_3912, at 96,481 to 96,780
_3426
EX28DRAFT_3427: EX28DRAFT_3427 - conserved hypothetical protein EF_0832/AHA_3913, at 96,803 to 97,579
_3427
EX28DRAFT_3428: EX28DRAFT_3428 - conserved hypothetical protein EF_0833/AHA_3914, at 97,593 to 98,234
_3428
Position (kb) 96
97 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 95.516 kb on - strand at 95.519 kb on + strand at 95.520 kb on - strand at 95.597 kb on + strand at 95.629 kb on - strand at 95.648 kb on + strand at 95.649 kb on - strand at 95.649 kb on - strand at 95.708 kb on + strand at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.835 kb on + strand, within EX28DRAFT_3424 at 95.851 kb on + strand, within EX28DRAFT_3424 at 95.851 kb on + strand, within EX28DRAFT_3424 at 95.891 kb on + strand, within EX28DRAFT_3424 at 96.127 kb on + strand at 96.128 kb on - strand at 96.326 kb on + strand, within EX28DRAFT_3425 at 96.333 kb on - strand, within EX28DRAFT_3425 at 96.333 kb on - strand, within EX28DRAFT_3425 at 96.344 kb on + strand, within EX28DRAFT_3425 at 96.415 kb on + strand, within EX28DRAFT_3425 at 96.447 kb on + strand at 96.448 kb on - strand at 96.476 kb on + strand at 96.476 kb on + strand at 96.476 kb on + strand at 96.742 kb on - strand, within EX28DRAFT_3426 at 96.782 kb on + strand at 96.789 kb on - strand at 96.810 kb on + strand at 96.811 kb on - strand at 96.811 kb on - strand at 96.822 kb on + strand at 97.528 kb on + strand at 97.529 kb on - strand at 97.577 kb on + strand at 97.578 kb on - strand at 97.590 kb on + strand at 97.590 kb on + strand at 97.591 kb on - strand at 97.591 kb on - strand at 97.604 kb on + strand at 97.604 kb on + strand at 97.605 kb on - strand at 97.618 kb on - strand at 97.686 kb on + strand, within EX28DRAFT_3428 at 97.686 kb on + strand, within EX28DRAFT_3428 at 97.686 kb on + strand, within EX28DRAFT_3428 at 97.686 kb on + strand, within EX28DRAFT_3428 at 97.687 kb on - strand, within EX28DRAFT_3428 at 97.687 kb on - strand, within EX28DRAFT_3428 at 97.689 kb on + strand, within EX28DRAFT_3428 at 97.689 kb on + strand, within EX28DRAFT_3428 at 97.690 kb on - strand, within EX28DRAFT_3428 at 97.690 kb on - strand, within EX28DRAFT_3428
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 95,516 - -0.4 95,519 + +0.5 95,520 - +0.4 95,597 + -0.5 95,629 - -0.7 95,648 + -0.4 95,649 - -0.5 95,649 - -0.0 95,708 + +0.2 95,835 + EX28DRAFT_3424 0.16 -1.5 95,835 + EX28DRAFT_3424 0.16 -0.2 95,835 + EX28DRAFT_3424 0.16 -0.6 95,851 + EX28DRAFT_3424 0.21 +0.4 95,851 + EX28DRAFT_3424 0.21 +0.0 95,891 + EX28DRAFT_3424 0.33 -0.3 96,127 + -2.1 96,128 - -1.9 96,326 + EX28DRAFT_3425 0.58 +0.3 96,333 - EX28DRAFT_3425 0.60 -0.7 96,333 - EX28DRAFT_3425 0.60 -0.3 96,344 + EX28DRAFT_3425 0.63 +0.2 96,415 + EX28DRAFT_3425 0.82 +1.1 96,447 + +0.8 96,448 - -0.5 96,476 + -0.5 96,476 + +0.5 96,476 + -1.7 96,742 - EX28DRAFT_3426 0.87 -1.0 96,782 + -0.6 96,789 - +0.2 96,810 + +0.3 96,811 - +0.1 96,811 - +0.6 96,822 + +0.0 97,528 + +0.1 97,529 - +0.3 97,577 + -0.2 97,578 - -0.7 97,590 + +0.1 97,590 + +0.4 97,591 - -1.0 97,591 - +0.1 97,604 + +0.0 97,604 + +0.5 97,605 - -3.8 97,618 - -0.6 97,686 + EX28DRAFT_3428 0.14 -0.3 97,686 + EX28DRAFT_3428 0.14 +0.5 97,686 + EX28DRAFT_3428 0.14 +0.9 97,686 + EX28DRAFT_3428 0.14 +0.2 97,687 - EX28DRAFT_3428 0.15 +0.1 97,687 - EX28DRAFT_3428 0.15 +2.1 97,689 + EX28DRAFT_3428 0.15 +0.1 97,689 + EX28DRAFT_3428 0.15 -0.8 97,690 - EX28DRAFT_3428 0.15 +0.2 97,690 - EX28DRAFT_3428 0.15 -0.0
Or see this region's nucleotide sequence