Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3001 and EX28DRAFT_3002 are separated by 90 nucleotides EX28DRAFT_3002 and EX28DRAFT_3003 overlap by 23 nucleotides EX28DRAFT_3003 and EX28DRAFT_3004 overlap by 4 nucleotides
EX28DRAFT_3001: EX28DRAFT_3001 - nucleoside transporter, at 309,284 to 310,540
_3001
EX28DRAFT_3002: EX28DRAFT_3002 - triphosphoribosyl-dephospho-CoA synthase CitG, at 310,631 to 311,434
_3002
EX28DRAFT_3003: EX28DRAFT_3003 - holo-ACP synthase CitX, at 311,412 to 311,951
_3003
EX28DRAFT_3004: EX28DRAFT_3004 - citrate lyase, alpha subunit, at 311,948 to 313,471
_3004
Position (kb) 310
311
312 Strain fitness (log2 ratio) -2
-1
0
1 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.635 kb on + strand, within EX28DRAFT_3001 at 309.636 kb on - strand, within EX28DRAFT_3001 at 309.691 kb on + strand, within EX28DRAFT_3001 at 309.692 kb on - strand, within EX28DRAFT_3001 at 309.712 kb on + strand, within EX28DRAFT_3001 at 309.713 kb on - strand, within EX28DRAFT_3001 at 309.761 kb on - strand, within EX28DRAFT_3001 at 309.770 kb on + strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.771 kb on - strand, within EX28DRAFT_3001 at 309.838 kb on + strand, within EX28DRAFT_3001 at 309.838 kb on + strand, within EX28DRAFT_3001 at 309.899 kb on - strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 309.925 kb on + strand, within EX28DRAFT_3001 at 310.057 kb on + strand, within EX28DRAFT_3001 at 310.058 kb on - strand, within EX28DRAFT_3001 at 310.357 kb on + strand, within EX28DRAFT_3001 at 310.382 kb on - strand, within EX28DRAFT_3001 at 310.397 kb on + strand, within EX28DRAFT_3001 at 310.397 kb on + strand, within EX28DRAFT_3001 at 310.493 kb on + strand at 310.494 kb on - strand at 310.494 kb on - strand at 310.538 kb on + strand at 310.538 kb on + strand at 310.611 kb on + strand at 310.632 kb on + strand at 310.723 kb on + strand, within EX28DRAFT_3002 at 310.724 kb on - strand, within EX28DRAFT_3002 at 311.062 kb on + strand, within EX28DRAFT_3002 at 311.093 kb on + strand, within EX28DRAFT_3002 at 311.093 kb on + strand, within EX28DRAFT_3002 at 311.414 kb on - strand at 311.781 kb on - strand, within EX28DRAFT_3003 at 311.852 kb on - strand, within EX28DRAFT_3003
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 309,635 + EX28DRAFT_3001 0.28 -0.7 309,635 + EX28DRAFT_3001 0.28 -0.8 309,635 + EX28DRAFT_3001 0.28 -0.3 309,636 - EX28DRAFT_3001 0.28 -0.5 309,691 + EX28DRAFT_3001 0.32 +0.0 309,692 - EX28DRAFT_3001 0.32 +0.6 309,712 + EX28DRAFT_3001 0.34 +0.8 309,713 - EX28DRAFT_3001 0.34 +0.1 309,761 - EX28DRAFT_3001 0.38 -0.2 309,770 + EX28DRAFT_3001 0.39 -1.5 309,771 - EX28DRAFT_3001 0.39 -0.1 309,771 - EX28DRAFT_3001 0.39 -0.7 309,771 - EX28DRAFT_3001 0.39 +0.2 309,771 - EX28DRAFT_3001 0.39 -1.8 309,838 + EX28DRAFT_3001 0.44 -0.3 309,838 + EX28DRAFT_3001 0.44 +0.6 309,899 - EX28DRAFT_3001 0.49 +0.6 309,925 + EX28DRAFT_3001 0.51 +0.1 309,925 + EX28DRAFT_3001 0.51 -0.7 309,925 + EX28DRAFT_3001 0.51 -0.1 310,057 + EX28DRAFT_3001 0.61 -0.5 310,058 - EX28DRAFT_3001 0.62 -0.1 310,357 + EX28DRAFT_3001 0.85 +0.5 310,382 - EX28DRAFT_3001 0.87 +0.7 310,397 + EX28DRAFT_3001 0.89 +0.5 310,397 + EX28DRAFT_3001 0.89 +0.1 310,493 + +0.1 310,494 - +0.6 310,494 - -1.9 310,538 + -0.5 310,538 + +0.9 310,611 + +0.3 310,632 + +0.3 310,723 + EX28DRAFT_3002 0.11 -0.2 310,724 - EX28DRAFT_3002 0.12 -0.4 311,062 + EX28DRAFT_3002 0.54 -1.7 311,093 + EX28DRAFT_3002 0.57 -2.0 311,093 + EX28DRAFT_3002 0.57 -0.8 311,414 - +0.5 311,781 - EX28DRAFT_3003 0.68 -0.1 311,852 - EX28DRAFT_3003 0.81 -0.0
Or see this region's nucleotide sequence