Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2953 and EX28DRAFT_2954 are separated by 76 nucleotides EX28DRAFT_2954 and EX28DRAFT_2955 are separated by 70 nucleotides EX28DRAFT_2955 and EX28DRAFT_2956 are separated by 124 nucleotides EX28DRAFT_2956 and EX28DRAFT_2957 are separated by 23 nucleotides
EX28DRAFT_2953: EX28DRAFT_2953 - Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase, at 261,613 to 263,046
_2953
EX28DRAFT_2954: EX28DRAFT_2954 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 263,123 to 263,866
_2954
EX28DRAFT_2955: EX28DRAFT_2955 - glycine dehydrogenase (decarboxylating), at 263,937 to 266,810
_2955
EX28DRAFT_2956: EX28DRAFT_2956 - glycine cleavage system H protein, at 266,935 to 267,324
_2956
EX28DRAFT_2957: EX28DRAFT_2957 - glycine cleavage system T protein, at 267,348 to 268,442
_2957
Position (kb)
263
264
265
266
267 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 262.938 kb on + strand at 262.943 kb on - strand at 263.010 kb on + strand at 263.010 kb on + strand at 263.081 kb on + strand at 263.082 kb on - strand at 263.092 kb on + strand at 263.093 kb on - strand at 263.144 kb on + strand at 263.159 kb on + strand at 263.298 kb on + strand, within EX28DRAFT_2954 at 263.315 kb on + strand, within EX28DRAFT_2954 at 263.316 kb on - strand, within EX28DRAFT_2954 at 263.409 kb on + strand, within EX28DRAFT_2954 at 263.409 kb on + strand, within EX28DRAFT_2954 at 263.410 kb on - strand, within EX28DRAFT_2954 at 263.410 kb on - strand, within EX28DRAFT_2954 at 263.611 kb on + strand, within EX28DRAFT_2954 at 263.714 kb on - strand, within EX28DRAFT_2954 at 263.714 kb on - strand, within EX28DRAFT_2954 at 263.758 kb on + strand, within EX28DRAFT_2954 at 263.759 kb on - strand, within EX28DRAFT_2954 at 263.759 kb on - strand, within EX28DRAFT_2954 at 263.772 kb on + strand, within EX28DRAFT_2954 at 263.791 kb on - strand, within EX28DRAFT_2954 at 263.916 kb on + strand at 263.916 kb on + strand at 263.922 kb on - strand at 263.922 kb on - strand at 263.924 kb on + strand at 263.924 kb on + strand at 263.924 kb on + strand at 263.924 kb on + strand at 263.925 kb on - strand at 263.925 kb on - strand at 263.925 kb on - strand at 263.925 kb on - strand at 263.925 kb on - strand at 263.935 kb on + strand at 263.936 kb on - strand at 263.936 kb on - strand at 263.936 kb on - strand at 263.938 kb on + strand at 263.939 kb on - strand at 263.944 kb on + strand at 263.990 kb on - strand at 264.068 kb on - strand at 264.400 kb on + strand, within EX28DRAFT_2955 at 264.401 kb on - strand, within EX28DRAFT_2955 at 264.401 kb on - strand, within EX28DRAFT_2955 at 264.401 kb on - strand, within EX28DRAFT_2955 at 264.439 kb on + strand, within EX28DRAFT_2955 at 264.439 kb on + strand, within EX28DRAFT_2955 at 264.440 kb on - strand, within EX28DRAFT_2955 at 264.440 kb on - strand, within EX28DRAFT_2955 at 264.440 kb on - strand, within EX28DRAFT_2955 at 264.440 kb on - strand, within EX28DRAFT_2955 at 264.506 kb on - strand, within EX28DRAFT_2955 at 264.737 kb on + strand, within EX28DRAFT_2955 at 264.738 kb on - strand, within EX28DRAFT_2955 at 264.738 kb on - strand, within EX28DRAFT_2955 at 264.822 kb on - strand, within EX28DRAFT_2955 at 264.833 kb on - strand, within EX28DRAFT_2955 at 264.853 kb on + strand, within EX28DRAFT_2955 at 265.337 kb on + strand, within EX28DRAFT_2955 at 265.337 kb on + strand, within EX28DRAFT_2955 at 265.338 kb on - strand, within EX28DRAFT_2955 at 265.339 kb on + strand, within EX28DRAFT_2955 at 265.339 kb on + strand, within EX28DRAFT_2955 at 265.340 kb on - strand, within EX28DRAFT_2955 at 265.544 kb on + strand, within EX28DRAFT_2955 at 265.651 kb on + strand, within EX28DRAFT_2955 at 265.670 kb on + strand, within EX28DRAFT_2955 at 265.769 kb on - strand, within EX28DRAFT_2955 at 265.805 kb on + strand, within EX28DRAFT_2955 at 265.811 kb on + strand, within EX28DRAFT_2955 at 265.811 kb on + strand, within EX28DRAFT_2955 at 265.910 kb on + strand, within EX28DRAFT_2955 at 265.910 kb on + strand, within EX28DRAFT_2955 at 265.911 kb on - strand, within EX28DRAFT_2955 at 265.911 kb on - strand, within EX28DRAFT_2955 at 265.911 kb on - strand, within EX28DRAFT_2955 at 265.922 kb on + strand, within EX28DRAFT_2955 at 266.477 kb on - strand, within EX28DRAFT_2955 at 266.477 kb on - strand, within EX28DRAFT_2955 at 266.561 kb on - strand at 266.567 kb on + strand at 266.567 kb on + strand at 266.567 kb on + strand at 266.698 kb on - strand at 266.797 kb on - strand at 266.843 kb on - strand at 266.843 kb on - strand at 266.929 kb on - strand at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.113 kb on + strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.114 kb on - strand, within EX28DRAFT_2956 at 267.251 kb on + strand, within EX28DRAFT_2956 at 267.251 kb on + strand, within EX28DRAFT_2956 at 267.736 kb on + strand, within EX28DRAFT_2957 at 267.767 kb on + strand, within EX28DRAFT_2957
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 262,938 + -0.1 262,943 - +1.6 263,010 + -0.5 263,010 + -0.2 263,081 + +0.1 263,082 - -0.6 263,092 + -0.1 263,093 - -1.4 263,144 + +0.1 263,159 + +0.2 263,298 + EX28DRAFT_2954 0.24 -0.2 263,315 + EX28DRAFT_2954 0.26 -0.6 263,316 - EX28DRAFT_2954 0.26 -1.1 263,409 + EX28DRAFT_2954 0.38 +0.2 263,409 + EX28DRAFT_2954 0.38 +0.5 263,410 - EX28DRAFT_2954 0.39 -0.1 263,410 - EX28DRAFT_2954 0.39 -0.2 263,611 + EX28DRAFT_2954 0.66 -0.2 263,714 - EX28DRAFT_2954 0.79 -0.1 263,714 - EX28DRAFT_2954 0.79 +0.8 263,758 + EX28DRAFT_2954 0.85 +0.4 263,759 - EX28DRAFT_2954 0.85 +2.0 263,759 - EX28DRAFT_2954 0.85 -0.1 263,772 + EX28DRAFT_2954 0.87 +0.0 263,791 - EX28DRAFT_2954 0.90 +0.3 263,916 + +1.3 263,916 + -0.2 263,922 - -1.1 263,922 - +0.3 263,924 + -0.1 263,924 + +0.8 263,924 + -0.3 263,924 + -0.4 263,925 - +0.4 263,925 - -0.8 263,925 - +0.1 263,925 - +0.4 263,925 - -0.2 263,935 + -0.9 263,936 - +0.0 263,936 - +0.8 263,936 - +0.2 263,938 + +0.6 263,939 - -0.0 263,944 + +0.1 263,990 - -3.3 264,068 - -1.0 264,400 + EX28DRAFT_2955 0.16 -1.2 264,401 - EX28DRAFT_2955 0.16 +0.7 264,401 - EX28DRAFT_2955 0.16 -0.9 264,401 - EX28DRAFT_2955 0.16 -0.9 264,439 + EX28DRAFT_2955 0.17 +0.5 264,439 + EX28DRAFT_2955 0.17 +0.0 264,440 - EX28DRAFT_2955 0.18 +0.6 264,440 - EX28DRAFT_2955 0.18 -0.8 264,440 - EX28DRAFT_2955 0.18 -0.7 264,440 - EX28DRAFT_2955 0.18 -0.6 264,506 - EX28DRAFT_2955 0.20 -0.2 264,737 + EX28DRAFT_2955 0.28 -0.6 264,738 - EX28DRAFT_2955 0.28 -0.2 264,738 - EX28DRAFT_2955 0.28 -0.7 264,822 - EX28DRAFT_2955 0.31 -0.9 264,833 - EX28DRAFT_2955 0.31 -0.4 264,853 + EX28DRAFT_2955 0.32 -0.6 265,337 + EX28DRAFT_2955 0.49 -0.8 265,337 + EX28DRAFT_2955 0.49 -0.6 265,338 - EX28DRAFT_2955 0.49 -0.7 265,339 + EX28DRAFT_2955 0.49 -1.1 265,339 + EX28DRAFT_2955 0.49 -1.6 265,340 - EX28DRAFT_2955 0.49 -0.7 265,544 + EX28DRAFT_2955 0.56 -0.4 265,651 + EX28DRAFT_2955 0.60 -0.4 265,670 + EX28DRAFT_2955 0.60 +0.3 265,769 - EX28DRAFT_2955 0.64 -0.5 265,805 + EX28DRAFT_2955 0.65 -2.5 265,811 + EX28DRAFT_2955 0.65 +0.3 265,811 + EX28DRAFT_2955 0.65 -0.1 265,910 + EX28DRAFT_2955 0.69 +0.5 265,910 + EX28DRAFT_2955 0.69 -0.9 265,911 - EX28DRAFT_2955 0.69 +1.3 265,911 - EX28DRAFT_2955 0.69 +0.5 265,911 - EX28DRAFT_2955 0.69 -0.8 265,922 + EX28DRAFT_2955 0.69 -0.7 266,477 - EX28DRAFT_2955 0.88 -1.1 266,477 - EX28DRAFT_2955 0.88 -0.7 266,561 - -0.7 266,567 + -0.6 266,567 + -1.0 266,567 + -0.5 266,698 - +0.7 266,797 - -1.1 266,843 - -0.4 266,843 - +0.2 266,929 - -1.9 267,113 + EX28DRAFT_2956 0.46 -0.6 267,113 + EX28DRAFT_2956 0.46 +0.3 267,113 + EX28DRAFT_2956 0.46 -0.6 267,113 + EX28DRAFT_2956 0.46 -0.7 267,113 + EX28DRAFT_2956 0.46 -0.4 267,113 + EX28DRAFT_2956 0.46 -1.0 267,113 + EX28DRAFT_2956 0.46 -0.9 267,114 - EX28DRAFT_2956 0.46 -0.3 267,114 - EX28DRAFT_2956 0.46 -0.7 267,114 - EX28DRAFT_2956 0.46 -0.4 267,114 - EX28DRAFT_2956 0.46 +0.3 267,251 + EX28DRAFT_2956 0.81 +1.3 267,251 + EX28DRAFT_2956 0.81 -0.7 267,736 + EX28DRAFT_2957 0.35 -1.0 267,767 + EX28DRAFT_2957 0.38 -0.5
Or see this region's nucleotide sequence