Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2544 and EX28DRAFT_2545 are separated by 32 nucleotides EX28DRAFT_2545 and EX28DRAFT_2546 are separated by 121 nucleotides EX28DRAFT_2546 and EX28DRAFT_2547 overlap by 38 nucleotides EX28DRAFT_2547 and EX28DRAFT_2548 are separated by 67 nucleotides
EX28DRAFT_2544: EX28DRAFT_2544 - Arabinose efflux permease, at 2,576,565 to 2,577,926
_2544
EX28DRAFT_2545: EX28DRAFT_2545 - Uncharacterized protein conserved in bacteria, at 2,577,959 to 2,578,450
_2545
EX28DRAFT_2546: EX28DRAFT_2546 - 2-dehydropantoate 2-reductase, at 2,578,572 to 2,579,483
_2546
EX28DRAFT_2547: EX28DRAFT_2547 - DJ-1 family protein, at 2,579,446 to 2,580,036
_2547
EX28DRAFT_2548: EX28DRAFT_2548 - tRNA sulfurtransferase ThiI/thiazole biosynthesis domain, at 2,580,104 to 2,581,552
_2548
Position (kb)
2578
2579
2580 Strain fitness (log2 ratio)
-1
0
1 at 2577.591 kb on + strand, within EX28DRAFT_2544 at 2577.592 kb on - strand, within EX28DRAFT_2544 at 2577.741 kb on + strand, within EX28DRAFT_2544 at 2577.797 kb on + strand at 2577.798 kb on - strand at 2577.874 kb on + strand at 2577.874 kb on + strand at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.093 kb on + strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.109 kb on - strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.111 kb on + strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.112 kb on - strand, within EX28DRAFT_2545 at 2578.307 kb on - strand, within EX28DRAFT_2545 at 2578.484 kb on + strand at 2578.519 kb on + strand at 2578.525 kb on + strand at 2578.526 kb on - strand at 2578.527 kb on + strand at 2578.528 kb on - strand at 2578.531 kb on + strand at 2578.531 kb on + strand at 2578.532 kb on - strand at 2578.678 kb on - strand, within EX28DRAFT_2546 at 2578.772 kb on - strand, within EX28DRAFT_2546 at 2579.017 kb on - strand, within EX28DRAFT_2546 at 2579.234 kb on + strand, within EX28DRAFT_2546 at 2579.235 kb on - strand, within EX28DRAFT_2546 at 2579.259 kb on + strand, within EX28DRAFT_2546 at 2579.260 kb on - strand, within EX28DRAFT_2546 at 2579.295 kb on - strand, within EX28DRAFT_2546 at 2579.432 kb on + strand at 2579.446 kb on - strand at 2579.446 kb on - strand at 2579.506 kb on - strand, within EX28DRAFT_2547 at 2579.980 kb on + strand at 2580.019 kb on + strand at 2580.025 kb on + strand at 2580.025 kb on + strand at 2580.025 kb on + strand at 2580.026 kb on - strand at 2580.028 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.034 kb on + strand at 2580.035 kb on - strand at 2580.035 kb on - strand at 2580.035 kb on - strand at 2580.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,577,591 + EX28DRAFT_2544 0.75 -0.8 2,577,592 - EX28DRAFT_2544 0.75 -0.1 2,577,741 + EX28DRAFT_2544 0.86 +0.6 2,577,797 + -0.9 2,577,798 - -0.1 2,577,874 + -0.7 2,577,874 + +1.2 2,578,093 + EX28DRAFT_2545 0.27 -0.5 2,578,093 + EX28DRAFT_2545 0.27 -0.7 2,578,109 - EX28DRAFT_2545 0.30 -0.1 2,578,109 - EX28DRAFT_2545 0.30 +1.0 2,578,111 + EX28DRAFT_2545 0.31 +1.5 2,578,111 + EX28DRAFT_2545 0.31 +1.2 2,578,111 + EX28DRAFT_2545 0.31 -1.0 2,578,112 - EX28DRAFT_2545 0.31 +0.2 2,578,112 - EX28DRAFT_2545 0.31 +0.1 2,578,307 - EX28DRAFT_2545 0.71 -0.5 2,578,484 + -0.5 2,578,519 + +0.3 2,578,525 + -0.3 2,578,526 - +0.5 2,578,527 + -0.8 2,578,528 - -0.3 2,578,531 + +0.3 2,578,531 + -0.4 2,578,532 - -0.1 2,578,678 - EX28DRAFT_2546 0.12 -0.9 2,578,772 - EX28DRAFT_2546 0.22 -0.3 2,579,017 - EX28DRAFT_2546 0.49 -0.7 2,579,234 + EX28DRAFT_2546 0.73 -0.1 2,579,235 - EX28DRAFT_2546 0.73 -1.6 2,579,259 + EX28DRAFT_2546 0.75 -0.3 2,579,260 - EX28DRAFT_2546 0.75 -1.6 2,579,295 - EX28DRAFT_2546 0.79 -0.7 2,579,432 + -0.6 2,579,446 - -1.6 2,579,446 - -1.0 2,579,506 - EX28DRAFT_2547 0.10 +0.5 2,579,980 + -0.4 2,580,019 + -0.5 2,580,025 + +0.1 2,580,025 + -0.0 2,580,025 + +1.0 2,580,026 - +1.5 2,580,028 + +0.0 2,580,034 + -0.4 2,580,034 + -0.5 2,580,034 + -0.3 2,580,034 + +0.7 2,580,035 - -0.5 2,580,035 - +0.2 2,580,035 - +0.3 2,580,106 - -0.1
Or see this region's nucleotide sequence