Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2529 and EX28DRAFT_2530 are separated by 212 nucleotides EX28DRAFT_2530 and EX28DRAFT_2531 are separated by 210 nucleotides EX28DRAFT_2531 and EX28DRAFT_2532 are separated by 69 nucleotides EX28DRAFT_2532 and EX28DRAFT_2533 are separated by 19 nucleotides
EX28DRAFT_2529: EX28DRAFT_2529 - Parvulin-like peptidyl-prolyl isomerase, at 2,559,413 to 2,561,284
_2529
EX28DRAFT_2530: EX28DRAFT_2530 - Bacterial nucleoid DNA-binding protein, at 2,561,497 to 2,561,769
_2530
EX28DRAFT_2531: EX28DRAFT_2531 - endopeptidase La, at 2,561,980 to 2,564,334
_2531
EX28DRAFT_2532: EX28DRAFT_2532 - RNA, at 2,564,404 to 2,564,497
_2532
EX28DRAFT_2533: EX28DRAFT_2533 - endopeptidase Clp ATP-binding regulatory subunit (clpX), at 2,564,517 to 2,565,791
_2533
Position (kb)
2561
2562
2563
2564
2565 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2561.163 kb on + strand at 2561.188 kb on - strand at 2561.271 kb on - strand at 2561.290 kb on - strand at 2561.362 kb on - strand at 2561.465 kb on + strand at 2561.466 kb on - strand at 2561.524 kb on - strand at 2561.686 kb on + strand, within EX28DRAFT_2530 at 2561.695 kb on + strand, within EX28DRAFT_2530 at 2561.788 kb on - strand at 2561.795 kb on - strand at 2561.795 kb on - strand at 2561.836 kb on + strand at 2561.858 kb on + strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.859 kb on - strand at 2561.886 kb on - strand at 2561.886 kb on - strand at 2561.886 kb on - strand at 2561.898 kb on - strand at 2561.902 kb on + strand at 2561.902 kb on + strand at 2561.906 kb on + strand at 2561.907 kb on - strand at 2561.908 kb on + strand at 2561.940 kb on + strand at 2561.941 kb on - strand at 2561.955 kb on + strand at 2563.205 kb on + strand, within EX28DRAFT_2531 at 2563.205 kb on + strand, within EX28DRAFT_2531 at 2563.206 kb on - strand, within EX28DRAFT_2531 at 2563.209 kb on + strand, within EX28DRAFT_2531 at 2563.210 kb on - strand, within EX28DRAFT_2531 at 2563.210 kb on - strand, within EX28DRAFT_2531 at 2563.989 kb on - strand, within EX28DRAFT_2531 at 2564.035 kb on - strand, within EX28DRAFT_2531 at 2564.352 kb on - strand at 2564.401 kb on - strand at 2564.401 kb on - strand at 2564.408 kb on - strand at 2564.408 kb on - strand at 2564.415 kb on - strand, within EX28DRAFT_2532 at 2564.415 kb on - strand, within EX28DRAFT_2532 at 2564.416 kb on + strand, within EX28DRAFT_2532 at 2564.489 kb on - strand at 2565.273 kb on + strand, within EX28DRAFT_2533 at 2565.273 kb on + strand, within EX28DRAFT_2533
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,561,163 + -0.2 2,561,188 - +0.2 2,561,271 - -0.1 2,561,290 - -0.4 2,561,362 - +0.7 2,561,465 + -0.1 2,561,466 - -0.6 2,561,524 - +0.1 2,561,686 + EX28DRAFT_2530 0.69 +0.0 2,561,695 + EX28DRAFT_2530 0.73 +0.1 2,561,788 - +0.0 2,561,795 - -0.3 2,561,795 - +0.2 2,561,836 + -0.3 2,561,858 + -0.7 2,561,859 - +0.6 2,561,859 - +0.3 2,561,859 - +0.3 2,561,859 - -2.3 2,561,886 - +0.2 2,561,886 - -0.4 2,561,886 - -0.2 2,561,898 - -0.5 2,561,902 + +0.0 2,561,902 + -0.1 2,561,906 + -0.1 2,561,907 - +0.1 2,561,908 + +0.4 2,561,940 + -0.1 2,561,941 - -0.6 2,561,955 + +0.3 2,563,205 + EX28DRAFT_2531 0.52 -1.1 2,563,205 + EX28DRAFT_2531 0.52 -2.6 2,563,206 - EX28DRAFT_2531 0.52 +0.2 2,563,209 + EX28DRAFT_2531 0.52 -1.8 2,563,210 - EX28DRAFT_2531 0.52 -0.4 2,563,210 - EX28DRAFT_2531 0.52 -3.3 2,563,989 - EX28DRAFT_2531 0.85 -3.6 2,564,035 - EX28DRAFT_2531 0.87 -0.4 2,564,352 - +1.0 2,564,401 - -2.4 2,564,401 - +1.2 2,564,408 - -0.3 2,564,408 - +0.2 2,564,415 - EX28DRAFT_2532 0.12 -1.1 2,564,415 - EX28DRAFT_2532 0.12 -0.5 2,564,416 + EX28DRAFT_2532 0.13 -1.4 2,564,489 - -0.5 2,565,273 + EX28DRAFT_2533 0.59 -1.0 2,565,273 + EX28DRAFT_2533 0.59 +1.2
Or see this region's nucleotide sequence