Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2266 and EX28DRAFT_2267 are separated by 266 nucleotides EX28DRAFT_2267 and EX28DRAFT_2268 are separated by 15 nucleotides EX28DRAFT_2268 and EX28DRAFT_2269 overlap by 1 nucleotides EX28DRAFT_2269 and EX28DRAFT_2270 overlap by 7 nucleotides
EX28DRAFT_2266: EX28DRAFT_2266 - 2-oxoglutarate dehydrogenase, E1 component, at 2,288,679 to 2,291,486
_2266
EX28DRAFT_2267: EX28DRAFT_2267 - succinate dehydrogenase and fumarate reductase iron-sulfur protein, at 2,291,753 to 2,292,469
_2267
EX28DRAFT_2268: EX28DRAFT_2268 - succinate dehydrogenase subunit A (EC 1.3.5.1), at 2,292,485 to 2,294,251
_2268
EX28DRAFT_2269: EX28DRAFT_2269 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 2,294,251 to 2,294,598
_2269
EX28DRAFT_2270: EX28DRAFT_2270 - succinate dehydrogenase subunit C (EC 1.3.5.1), at 2,294,592 to 2,295,071
_2270
Position (kb)
2292
2293
2294
2295 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2291.507 kb on + strand at 2291.508 kb on - strand at 2291.549 kb on + strand at 2291.549 kb on + strand at 2291.550 kb on - strand at 2291.599 kb on + strand at 2291.600 kb on - strand at 2291.621 kb on - strand at 2291.755 kb on - strand at 2291.782 kb on + strand at 2291.861 kb on + strand, within EX28DRAFT_2267 at 2291.862 kb on - strand, within EX28DRAFT_2267 at 2292.022 kb on + strand, within EX28DRAFT_2267 at 2292.022 kb on + strand, within EX28DRAFT_2267 at 2292.031 kb on - strand, within EX28DRAFT_2267 at 2292.328 kb on + strand, within EX28DRAFT_2267 at 2292.353 kb on + strand, within EX28DRAFT_2267 at 2292.486 kb on + strand at 2292.493 kb on + strand at 2292.701 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2292.854 kb on - strand, within EX28DRAFT_2268 at 2293.070 kb on + strand, within EX28DRAFT_2268 at 2293.189 kb on - strand, within EX28DRAFT_2268 at 2293.425 kb on + strand, within EX28DRAFT_2268 at 2293.627 kb on - strand, within EX28DRAFT_2268 at 2293.631 kb on - strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.990 kb on + strand, within EX28DRAFT_2268 at 2293.991 kb on - strand, within EX28DRAFT_2268 at 2294.043 kb on + strand, within EX28DRAFT_2268 at 2294.044 kb on - strand, within EX28DRAFT_2268 at 2294.063 kb on - strand, within EX28DRAFT_2268 at 2294.216 kb on + strand at 2294.279 kb on + strand at 2294.280 kb on - strand at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.352 kb on - strand, within EX28DRAFT_2269 at 2294.502 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.504 kb on + strand, within EX28DRAFT_2269 at 2294.505 kb on - strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.513 kb on + strand, within EX28DRAFT_2269 at 2294.560 kb on + strand, within EX28DRAFT_2269 at 2294.601 kb on - strand at 2294.690 kb on - strand, within EX28DRAFT_2270 at 2295.107 kb on + strand at 2295.193 kb on - strand at 2295.196 kb on + strand at 2295.196 kb on + strand at 2295.197 kb on - strand at 2295.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,291,507 + -2.6 2,291,508 - -1.6 2,291,549 + -4.4 2,291,549 + -4.4 2,291,550 - -2.9 2,291,599 + -5.5 2,291,600 - -2.4 2,291,621 - -2.7 2,291,755 - +0.4 2,291,782 + -4.3 2,291,861 + EX28DRAFT_2267 0.15 -0.9 2,291,862 - EX28DRAFT_2267 0.15 -2.0 2,292,022 + EX28DRAFT_2267 0.38 -4.6 2,292,022 + EX28DRAFT_2267 0.38 -1.6 2,292,031 - EX28DRAFT_2267 0.39 -2.6 2,292,328 + EX28DRAFT_2267 0.80 -2.9 2,292,353 + EX28DRAFT_2267 0.84 -4.1 2,292,486 + -5.1 2,292,493 + -2.7 2,292,701 - EX28DRAFT_2268 0.12 -2.1 2,292,854 - EX28DRAFT_2268 0.21 -2.4 2,292,854 - EX28DRAFT_2268 0.21 -1.2 2,292,854 - EX28DRAFT_2268 0.21 -0.3 2,292,854 - EX28DRAFT_2268 0.21 +0.1 2,292,854 - EX28DRAFT_2268 0.21 -0.5 2,293,070 + EX28DRAFT_2268 0.33 -3.8 2,293,189 - EX28DRAFT_2268 0.40 -2.8 2,293,425 + EX28DRAFT_2268 0.53 -3.8 2,293,627 - EX28DRAFT_2268 0.65 -3.1 2,293,631 - EX28DRAFT_2268 0.65 -2.2 2,293,990 + EX28DRAFT_2268 0.85 -2.4 2,293,990 + EX28DRAFT_2268 0.85 -3.6 2,293,990 + EX28DRAFT_2268 0.85 -3.1 2,293,991 - EX28DRAFT_2268 0.85 -4.7 2,294,043 + EX28DRAFT_2268 0.88 -4.4 2,294,044 - EX28DRAFT_2268 0.88 -2.7 2,294,063 - EX28DRAFT_2268 0.89 +0.3 2,294,216 + -3.8 2,294,279 + -3.9 2,294,280 - -2.7 2,294,352 - EX28DRAFT_2269 0.29 -1.6 2,294,352 - EX28DRAFT_2269 0.29 -2.0 2,294,502 + EX28DRAFT_2269 0.72 -3.0 2,294,504 + EX28DRAFT_2269 0.73 -1.4 2,294,504 + EX28DRAFT_2269 0.73 -2.5 2,294,504 + EX28DRAFT_2269 0.73 -2.2 2,294,505 - EX28DRAFT_2269 0.73 -1.5 2,294,513 + EX28DRAFT_2269 0.75 -3.5 2,294,513 + EX28DRAFT_2269 0.75 -2.1 2,294,513 + EX28DRAFT_2269 0.75 -2.5 2,294,560 + EX28DRAFT_2269 0.89 -0.9 2,294,601 - -2.8 2,294,690 - EX28DRAFT_2270 0.20 -2.1 2,295,107 + -2.2 2,295,193 - -1.8 2,295,196 + -1.1 2,295,196 + -0.9 2,295,197 - -2.3 2,295,197 - -0.9
Or see this region's nucleotide sequence